A5044098186- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Pluripotent Stem Cells Research
- Food Quality and Safety Studies
- Agriculture, Soil, Plant Science
- DNA and Nucleic Acid Chemistry
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Nutrition, Health and Food Behavior
- Vitamin D Research Studies
- Radio Frequency Integrated Circuit Design
- Insect symbiosis and bacterial influences
- Plant Reproductive Biology
- Biotin and Related Studies
- Ecology and Conservation Studies
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Plant Virus Research Studies
- Advanced Power Amplifier Design
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Seed Germination and Physiology
Korea Research Institute of Bioscience and Biotechnology
2015-2023
Jeju National University
2023
Noble Research Institute
2016-2020
Kyungpook National University
2017-2019
Korea University of Science and Technology
2015-2018
Oak Ridge National Laboratory
2016-2017
Pohang University of Science and Technology
2007-2013
Yeungnam University
2012
Samsung (South Korea)
2009-2010
Kyung Hee University
2010
Abstract Although significant progress has been made in the development of vacuum‐deposited small‐molecule organic light‐emitting diodes (OLEDs), one most desired research goals is still to produce flexible displays by low‐cost solution processing. The solution‐processed OLEDs based on small molecules could potentially be a good approach but no intensive studies this topic have conducted so far. To fabricate high‐performance devices molecules, underlying nature produced films and must...
Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR associated protein 9 (Cas9) system allows effective gene modification through RNA-guided DNA targeting. The Cas9 has undergone a series of functional alterations from original active endonuclease to partially or completely deactivated Cas9. catalytically (dCas9) offers platform regulate transcriptional expression with addition activator repressor domains. We redesigned CRISPR/Cas9 activation by adding p65...
The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with allotetraploid (2n = 4x 36), a complexity which forms impediment to generating homozygous knock-out plants. Lignin, major component the plant cell wall and contributor cellulosic feedstock's recalcitrance decomposition, stands as barrier efficient biofuel production by limiting enzyme access polymers during...
Summary The plant U‐box (PUB) protein functions as an E3 ligase to poly‐ubiquitinate a target for its degradation or post‐translational modification. Here, we report functional roles OsPUB15 , which encodes cytosolic in the class‐II PUB family. Self‐ubiquitination assays showed that bacterially expressed MBP‐OsPUB15 has ubiquitin activity. A T‐DNA insertional mutation caused severe growth retardation and seedling‐lethal phenotype. Mutant seeds did not produce primary roots, their shoot...
Significance Physical dormancy, often called hardseededness, exists widely in higher plants. This adaptive trait allows plants to maintain a seed bank soil, and thus survive unfavorable environmental conditions. The molecular mechanism of physical dormancy is largely elusive. We used the model legume plant, Medicago truncatula, study this because species produces seeds with typical dormancy. By screening large M. truncatula population tagged retrotransposon, we isolated mutant that lost...
Abstract Main conclusion An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-driven Cas9 exhibits consistently high mutation efficiency in and M. truncatula. is a powerful genome editing technology that has been applied several crop species for trait improvement due to its simplicity, versatility, specificity. However, of truncatula (Mt) still challenging inconsistent. To analyze functionality two model dicot species, four different systems target phytoene desaturase ( PDS )...
Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system has been successfully used for genome editing many plant species. However, use of CRISPR/Cas9 gene knockout alfalfa still very challenging. Our initial single gRNA-CRISPR/Cas9 had low mutagenesis efficiency with no mutant phenotype. In order to develop optimized alfalfa, we...
Abstract The disruption of the retinal pigment epithelium (RPE), for example, through oxidative damage, is a common factor underlying age-related macular degeneration (AMD). Aberrant autophagy also contributes to AMD pathology, as maintains RPE homeostasis ensure blood–retinal barrier (BRB) integrity and protect photoreceptors. Thioredoxin-interacting protein (TXNIP) promotes cellular stress by inhibiting thioredoxin reducing capacity in turn inversely regulated reactive oxygen species...
Hole trapping: The hole transfer and trapping in a G-quadruplex has been investigated using laser flash photolysis pulse radiolysis. results show that the charge separation takes place efficiently between riboflavin G-bases because of excellent hole-trapping ability planar G-quartets.
Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with 5'-to-3' exonuclease Recombination J (RecJ) or GFP demonstrated that transfection pre-assembled ribonucleoprotein two into human plant cells resulted in greater targeted mutagenesis efficiency up 600% without noticeable increase effects. Improved activity fusion...
Pluripotent stem cells (PSCs) represent the most promising clinical source for regenerative medicine. However, given cellular heterogeneity within cultivation and safety concerns, development of specific efficient tools to isolate a pure population eliminate all residual undifferentiated PSCs from differentiated derivatives is prerequisite applications. In this study, we raised monoclonal antibody identified its target antigen as desmoglein-2 (DSG2). DSG2 co-localized with human PSC...
Binding geometry to DNA and the efficiency as a donor for energy transfer of various metallo- nonmetallo-porphyrins were investigated mainly by polarized light spectrscopy fluorescence measurements. Planar porphyrins including nonmetallo meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP), CuTMPyP, NiTMPyP produced large red-shift hypochromism in absorption spectrum negative circular dichroism (CD) Soret band suggesting that these intercalate between base-pairs expected. In intercalation...
Strongly luminescent and highly loaded CdSe quantum dot (QD)−silica composite films are prepared by a simple one-step method. QDs grown in situ as the silica matrix forms from spin-on-glass organosiloxane polymers. The nucleation growth of is directly observed using TEM. QD composites comprehensively characterized, which includes photoluminescence(PL) decay, PL efficiency, volume fraction. emission can span entire visible range be tuned preparation condition. very strong, reaching efficiency...
This paper presents a fully-integrated linear CMOS power amplifier (PA) with an adaptive gate bias circuit in Common-Gate (CG) amplifiers. The is proposed to achieve high linearity deep class-AB biasing of Common-Source (CS) stage. single stage PA including the fabricated using 0.18-μm RF technology. improves evolved universal terrestrial radio access adjacent channel leakage ratio (ACLR <sub xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Löchereinfang: Der Transfer und das Einfangen von Löchern in einem G-Quadruplex wurden mit Laserblitzphotolyse Pulsradiolyse untersucht. Die Ergebnisse zeigen, dass im zwischen Riboflavin den G-Basen eine effiziente Ladungstrennung auftritt, weil die planaren G-Quartette Löcher exzellent einfangen können (siehe Bild).
Ensuring adequate levels of vitamin D3 in the human diet has long been an important objective crop breeding, as most crops have extremely low this compound. To address challenge, we employed CRISPR-Cas9 gene editing system tomatoes to induce loss-of-function mutations one two DWARF5 genes, a homologue dehydrocholesterol Δ7-reductase gene. Lines with knocked out SIDWF5A exhibited visually indistinguishable phenotypes, yet remarkably accumulated provitamin high 6 μg/g dry weight (DW) red...