A5068128114- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Postharvest Quality and Shelf Life Management
- Plant tissue culture and regeneration
- Telomeres, Telomerase, and Senescence
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Neonatal and fetal brain pathology
- Plant biochemistry and biosynthesis
- Plant responses to water stress
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Neonatal Respiratory Health Research
- Plant Physiology and Cultivation Studies
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Respiratory viral infections research
- Anesthesia and Neurotoxicity Research
- CRISPR and Genetic Engineering
- GABA and Rice Research
Yonsei University
2016-2025
Catholic University of Daegu
2010-2019
Daegu Catholic University Medical Center
2017-2018
Wonju Severance Christian Hospital
2011-2012
Korea Institute of Science & Technology Information
2011-2012
Taegu Science University
2011
Pohang University of Science and Technology
2003-2009
Dongguk University
1996-2009
University of Tsukuba
2009
Institute of Applied Biochemistry
2009
Doil Choi and colleagues report the genome sequence of hot pepper, Capsicum annuum, as well resequencing two cultivated peppers a wild species, chinense. Comparative genomic analysis across Solanaceae provides insights into expansion, pungency, ripening disease resistance in peppers. Hot pepper (Capsicum annuum), one oldest domesticated crops Americas, is most widely grown spice crop world. We whole-genome sequencing assembly (Mexican landrace annuum cv. CM334) at 186.6× coverage. also de...
Abstract Ubiquitination is involved in a variety of biological processes, but the exact role ubiquitination abiotic responses not clearly understood higher plants. Here, we investigated Rma1H1, hot pepper (Capsicum annuum) homolog human RING membrane-anchor 1 E3 ubiquitin (Ub) ligase. Bacterially expressed Rma1H1 displayed Ub ligase activity vitro. was rapidly induced by various stresses, including dehydration, and its overexpression transgenic Arabidopsis thaliana plants conferred strongly...
Abstract We analyzed 6,749 lines tagged by the gene trap vector pGA2707. This resulted in isolation of 3,793 genomic sequences flanking T-DNA. Among insertions, 1,846 T-DNAs were integrated into genic regions, and 1,864 located intergenic regions. Frequencies also higher at beginning end coding regions upstream near ATG start codon. The overall GC content insertion sites was close to that measured from entire rice (Oryza sativa) genome. Functional classification these genes showed a...
Abstract Ubiquitination is involved in diverse cellular processes higher plants. In this report, we describe Arabidopsis thaliana PUB22 and PUB23, two homologous U-box–containing E3 ubiquitin (Ub) ligases. The PUB23 genes were rapidly coordinately induced by abiotic stresses but not abscisic acid. PUB22- PUB23-overexpressing transgenic plants hypersensitive to drought stress. By contrast, loss-of-function pub22 pub23 mutant significantly more drought-tolerant, a double displayed even greater...
Xyloglucan endotransglucosylase/hydrolase (XTH) has been recognized as a cell wall‐modifying enzyme, participating in the diverse physiological roles. From water‐stressed hot pepper plants, we isolated three different cDNA clones (pCaXTH1, pCaXTH2, and pCaXTH3) that encode XTH homologs. RT‐PCR analysis showed CaXTH mRNAs were concomitantly induced by broad spectrum of abiotic stresses, including drought, high salinity cold temperature, response to stress hormone ethylene, suggesting their...
Recent findings suggest a pivotal role for mitochondria-associated hexokinase in the regulation of apoptosis animal cells. In this study, virus-induced gene silencing (VIGS) hexokinase-encoding Hxk1 caused necrotic lesions on leaves, abnormal leaf morphology, and retarded plant growth Nicotiana benthamiana. was associated with mitochondria, association required N-terminal membrane anchor. VIGS reduced cellular glucose-phosphorylating activity to approximately 31% control levels without...
Abstract Ubiquitination is a eukaryotic posttranslational protein modification that mediated by the cascade of E1, E2, and E3 ubiquitin (Ub) ligases involved in regulating numerous cellular functions. In this study, we obtained 100 different Arabidopsis (Arabidopsis thaliana) T-DNA insertion mutant plants which RING Ub ligase genes were suppressed monitored their phenotypes presence exogenous abscisic acid (ABA), plant stress hormone. One these loss-of-function mutants displayed...
Abstract AtPUB18 and AtPUB19 are homologous U-box E3 ubiquitin ligases in Arabidopsis (Arabidopsis thaliana). is a negative regulator of abscisic acid (ABA)-mediated drought responses, whereas the role responses unknown. Here, loss-of-function overexpression tests identified as ABA-mediated stomatal closure water stress responses. The atpub18-2atpub19-3 double mutant line displayed more sensitivity to ABA enhanced tolerance than each single plant; therefore, agonistic. Stomatal was...
Abstract Really Interesting New Gene (RING) E3 ubiquitin ligases have been implicated in cellular responses to the stress hormone abscisic acid (ABA) as well environmental stresses higher plants. Here, an ABA-insensitive RING protein3 (atairp3) loss-of-function mutant line Arabidopsis (Arabidopsis thaliana) was isolated due its hyposensitivity ABA during germination stage compared with wild-type AtAIRP3 contains a single C3HC4-type motif, putative myristoylation site, and domain associated...
Summary The plant U‐box (PUB) protein functions as an E3 ligase to poly‐ubiquitinate a target for its degradation or post‐translational modification. Here, we report functional roles OsPUB15 , which encodes cytosolic in the class‐II PUB family. Self‐ubiquitination assays showed that bacterially expressed MBP‐OsPUB15 has ubiquitin activity. A T‐DNA insertional mutation caused severe growth retardation and seedling‐lethal phenotype. Mutant seeds did not produce primary roots, their shoot...
The U-box motif is a conserved domain found in the diverse isoforms of E3 ubiquitin ligase eukaryotes. From water-stressed hot pepper (Capsicum annuum L. cv Pukang) plants, we isolated C. putative protein 1 (CaPUB1), which encodes containing single its N-terminal region. In vitro ubiquitination and site-directed mutagenesis assays revealed that CaPUB1 possessed activity was indeed essential for enzyme activity. RNA gel-blot analysis showed mRNA induced rapidly by broad spectrum abiotic...
The ubiquitin (Ub)-26S proteasome pathway is implicated in various cellular processes higher plants. AtAIRP1, a C3H2C3-type RING (for Really Interesting New Gene) E3 Ub ligase, positive regulator the Arabidopsis (Arabidopsis thaliana) abscisic acid (ABA)-dependent drought response. Here, AtAIRP2 ABA-insensitive protein 2) gene was identified and characterized. encodes cytosolic C3HC4-type ligase whose expression markedly induced by ABA dehydration stress. Thus, belongs to different subclass...
Summary Prohibitin, which consists of two subunits PHB1 and PHB2, plays a role in cell‐cycle progression, senescence, apoptosis, maintenance mitochondrial function mammals yeast. In this study, we examined the prohibitins plants by using virus‐induced gene silencing (VIGS) prohibitin subunit genes Nicotiana benthamiana , designated NbPHB1 NbPHB2 . were targeted to mitochondria, their expression was suppressed during senescence. VIGS caused severe growth inhibition, leaf yellowing symptoms...
Abstract Lipid droplets (LDs) act as repositories for fatty acids and sterols, which are used various cellular processes such energy production membrane hormone synthesis. LD-associated proteins play important roles in seed development germination, but their functions postgermination growth not well understood. Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, SRP3) that share sequence identities with small rubber particle of the tree (Hevea brasiliensis). In this...
The Arabidopsis thaliana U-box E3 ligases PUB18/PUB19 and PUB22/PUB23 are negative regulators of drought stress responses. regulate the response in an abscisic acid (ABA)-dependent manner, whereas this ABA-independent manner. A major structural difference between is presence UND (U-box N-terminal domain). Here, we focused on elucidating molecular mechanism that mediates functional PUB18 PUB22 found UNDPUB18 was critically involved regulation ABA-mediated stomatal movements. Exo70B1, a...
AtATL78 is an Arabidopsis RING E3 ubiquitin ligase. RT‐PCR and promoter‐GUS assays revealed that was up‐regulated by cold stress down‐regulated drought. localized at the plasma‐membrane. Suppression of increased tolerance to but decreased Our data suggests a negative regulator response positive drought in . These results further suggest plays opposing roles responses.