- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- GABA and Rice Research
- Mycobacterium research and diagnosis
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Redox biology and oxidative stress
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Tuberculosis Research and Epidemiology
- Plant Disease Resistance and Genetics
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Insect Resistance and Genetics
- Infectious Diseases and Mycology
- Heavy Metal Exposure and Toxicity
- Insect-Plant Interactions and Control
- Photosynthetic Processes and Mechanisms
- Geotechnical Engineering and Underground Structures
- Hepatitis C virus research
- Hemoglobinopathies and Related Disorders
- CRISPR and Genetic Engineering
- Iron Metabolism and Disorders
- Plant tissue culture and regeneration
- Trace Elements in Health
Columbia University
2025
International Rice Research Institute
2015-2024
Kumoh National Institute of Technology
2022-2024
Nkmax (South Korea)
2022
Sookmyung Women's University
2022
Korea Institute of Ceramic Engineering and Technology
2022
Kyung Hee University
2011-2021
Zero to Three
2021
Samsung (South Korea)
2020
Korea Forest Service
2016
We have generated 47,932 T-DNA tag lines in japonica rice using activation-tagging vectors that contain tetramerized 35S enhancer sequences. To facilitate use of those lines, we isolated the genomic sequences flanking inserted via inverse polymerase chain reaction. For most performed four sets amplifications two different restriction enzymes toward both directions. In analyzing 41,234 obtained 27,621 sequence tags (FSTs), among which 12,505 were integrated into genic regions and 15,116...
Abstract We analyzed 6,749 lines tagged by the gene trap vector pGA2707. This resulted in isolation of 3,793 genomic sequences flanking T-DNA. Among insertions, 1,846 T-DNAs were integrated into genic regions, and 1,864 located intergenic regions. Frequencies also higher at beginning end coding regions upstream near ATG start codon. The overall GC content insertion sites was close to that measured from entire rice (Oryza sativa) genome. Functional classification these genes showed a...
Abstract Grass cell wall properties influence food, feed, and biofuel feedstock usage efficiency. The glucuronoarabinoxylan of grass walls is esterified with the phenylpropanoid-derived hydroxycinnamic acids ferulic acid (FA) para-coumaric (p-CA). Feruloyl esters undergo oxidative coupling neighboring phenylpropanoids on lignin. Examination rice (Oryza sativa) mutants in a grass-expanded -diverged clade BAHD acyl-coenzyme A-utilizing transferases identified four altered FA or p-CA contents....
RNA editing is the alteration of sequences via insertion, deletion and conversion nucleotides. In flowering plants, specific cytidine residues transcribed from organellar genomes are converted into uridines. Approximately 35 sites present in chloroplasts higher plants; six pentatricopeptide repeat genes involved have been identified Arabidopsis. However, although approximately 500 found mitochondrial RNAs only one gene Arabidopsis has reported to be such editing. Here, we rice mutants that...
Abstract Aldehyde dehydrogenases (ALDHs) catalyze the irreversible oxidation of a wide range reactive aldehydes to their corresponding carboxylic acids. Although proteins have been studied from various organisms and at different growth stages, roles in seed development not well elucidated. We obtained T-DNA insertional mutants OsALDH7, which is remarkably inducible by oxidative abiotic stresses. Interestingly, endosperms osaldh7 null accumulated brown pigments during desiccation storage....
Abstract Brown planthopper (BPH) is a phloem sap-sucking insect pest of rice which causes severe yield loss. We cloned the BPH18 gene from BPH-resistant introgression line derived wild species Oryza australiensis . Map-based cloning and complementation test revealed that encodes CC-NBS-NBS-LRR protein. has two NBS domains, unlike typical NBS-LRR proteins. The promoter:: GUS transgenic plants exhibited strong expression in vascular bundles leaf sheath, especially cells where BPH attacks....
Trithorax group proteins are chromatin-remodeling factors that activate target gene expression by antagonistically functioning against the Polycomb group. In Arabidopsis (Arabidopsis thaliana), protein1 (ATX1) regulates flowering time and floral organ identity. Here, we observed suppression of Oryza sativa Trithorax1 (OsTrx1), an ortholog ATX1, delayed in rice (Oryza sativa). Because delay occurred only under long-day conditions, evaluated signal pathways specifically function conditions....
Flowering is exquisitely regulated by both promotive and inhibitory factors. Molecular genetic studies with Arabidopsis have verified several epigenetic repressors that regulate flowering time. However, the roles of chromatin remodeling factors in developmental processes not been well explored Oryza sativa (rice). We identified a factor OsVIL2 (O. VIN3-LIKE 2) promotes flowering. contains plant homeodomain (PHD) finger, which conserved motif histone binding proteins. Insertion mutations...
The pollen wall consists of an exine and intine. mechanism underlying its formation is not well understood. Glycosyltransferases catalyze the modification biological molecules by attaching a single or multiple sugars play key roles in wide range processes. We examined role GLYCOSYLTRANSFERASE1 (OsGT1) development rice (Oryza sativa). This gene highly expressed mature pollen, plants containing alleles caused transfer DNA insertion do produce homozygous progeny. Reciprocal crosses between...
Although susceptibility to seed shattering causes severe yield loss during cereal crop harvest, it is an adaptive trait for dispersal in wild plants. We previously identified a recessive locus, sh-h, from the rice mutant line Hsh that carries enhanced abscission layer. Here, we further mapped sh-h 34-kb region on chromosome 7 by analyzing 240 F(2) plants and five F(3) lines cross between Blue&Gundil. had point mutation at 3' splice site of seventh intron within LOC_Os07g10690, causing 15-bp...
Rice is one of the major staple foods in world, especially developing countries Asia. Its consumption as a dietary source also increasing Africa. To meet demand for rice to feed human population, yield essential. Improving genetic potential ideal solution. It imperative introduce identified yield-enhancing gene(s) into modern cultivars rapid improvement through marker-assisted breeding. We report development PCR-gel-based markers eight yield-related functional genes (Gn1a, OsSPL14, SCM2,...
Cultivated rice (Oryza sativa L.) is very sensitive to salt stress. So far a few landraces have been identified as source of tolerance and utilized in improvement. These tolerant lines primarily use Na+ exclusion mechanism root which removes from the xylem stream by membrane K+ transporters, resulted low accumulation shoot. Identification new donor conferring high imperative. Wild relatives having wide genetic diversity are regarded potential for crop However, they less exploited against...
Rice (Oryza sativa L.) is the staple food for more than 3.5 billion people, mainly in Asia. Brown planthopper (BPH) one of most destructive insect pests rice that limits production. Host-plant resistance efficient ways to overcome BPH damage crop.BPH bioassay studies from 2009 2015 conducted India and at International Research Institute (IRRI), Philippines, revealed cultivar CR2711-76 developed National (NRRI), Cuttack, India, showed stable broad-spectrum several populations Philippines...
SUMMARY Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice ( Oryza sativa ), concentration trans ‐zeatin, an active cytokinin, was high in leaves during decreased rapidly upon induction florigen expression, suggesting that this hormone is involved regulation phase. To elucidate whether exogenous cytokinin application influences length phase, we applied 6‐benzylaminopurine (BAP) to plants at various developmental stages. Our treatment delayed...
Abstract Rice yield potential has been stagnant since the Green Revolution in late 1960s, especially tropical rice cultivars. We evaluated effect of two major genes that regulate grain number, Gn1a/OsCKX2 and IPA1/WFP/OsSPL14 , elite indica cultivar backgrounds. The yield-positive Gn1a -type 3 OsSPL14 WFP alleles were introgressed respectively through marker-assisted selection (MAS). numbers per panicle (GNPP) compared between recipient allele donor groups using segregating plants BC F 2...
To counter the rising incidence of diabetes and to meet daily protein needs, we created low glycemic index (GI) rice varieties with content (PC) surpassing 14%. In development recombinant inbred lines using Samba Mahsuri IR36 amylose extender (IR36ae) as parental lines, identified quantitative trait loci genes associated GI, high (AC), PC. By integrating genetic techniques classification models, this comprehensive approach candidate on chromosome 2 ( qGI2.1/qAC2.1 spanning region from 18.62...
The aim of this prospective clinical cohort study is to evaluate how the use ovate pontic alongside alveolar ridge preservation (ARP) contributes soft tissue when placed immediately post-extraction into a socket with or without intact buccal bone plate in esthetic zone. Twenty-three patients non-restorable tooth maxillary zone bound by natural adjacent teeth were recruited for study. At time extraction, assigned three groups, Group A (type I ARP), B II and C ARP membrane). Following flapless...