A5078649797- Pregnancy and preeclampsia studies
- Plant Molecular Biology Research
- Single-cell and spatial transcriptomics
- Birth, Development, and Health
- CAR-T cell therapy research
- Plant Stress Responses and Tolerance
- Cell Image Analysis Techniques
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Cutaneous lymphoproliferative disorders research
- Scientific Computing and Data Management
- Astronomical Observations and Instrumentation
- Electron and X-Ray Spectroscopy Techniques
- Advanced X-ray Imaging Techniques
- Photosynthetic Processes and Mechanisms
- Prenatal Screening and Diagnostics
- Advanced Electron Microscopy Techniques and Applications
- Plant tissue culture and regeneration
- Species Distribution and Climate Change
- Machine Learning in Healthcare
- Renal and related cancers
- Plant Reproductive Biology
- Artificial Intelligence in Healthcare
- T-cell and B-cell Immunology
- Preterm Birth and Chorioamnionitis
Wellcome Sanger Institute
2023-2024
Diamond Light Source
2020-2024
Newcastle University
2022-2024
Kyung Hee University
2019-2023
University of Auckland
2015-2019
Human prenatal skin is populated by innate immune cells, including macrophages, but whether they act solely in immunity or have additional functions morphogenesis unclear. Here we assembled a comprehensive multi-omics reference atlas of human (7-17 post-conception weeks), combining single-cell and spatial transcriptomics data, to characterize the microanatomical tissue niches skin. This revealed that crosstalk between non-immune cells underpins formation hair follicles, implicated scarless...
SUMMARY Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice ( Oryza sativa ), concentration trans ‐zeatin, an active cytokinin, was high in leaves during decreased rapidly upon induction florigen expression, suggesting that this hormone is involved regulation phase. To elucidate whether exogenous cytokinin application influences length phase, we applied 6‐benzylaminopurine (BAP) to plants at various developmental stages. Our treatment delayed...
Sucrose controls various developmental and metabolic processes in plants. It also functions as a signaling molecule the synthesis of carbohydrates, storage proteins, anthocyanins, well floral induction defense response. We found that sucrose preferentially induced OsWRKY7 , whereas other sugars (such mannitol, glucose, fructose, galactose, maltose) did not have same effect. A hexokinase inhibitor mannoheptulose block effect sucrose, which is consequently thought to function directly. MG132...
Fetal growth restriction (FGR) affects 5-10% of pregnancies, leading to clinically significant fetal morbidity and mortality. FGR placentae frequently exhibit poor vascular branching, but the mechanisms driving this are poorly understood. We hypothesize that structural malformation at organ level alters microvascular shear stress, impairing angiogenesis. A computational model placental vasculature predicted elevated micro-vascular stress in (0.2 Pa severe vs 0.05 normal placentae)....
Multi-scale structural assessment of biological soft tissue is challenging but essential to gain insight into structure–function relationships tissue/organ. Using the human placenta as an example, this study brings together sophisticated sample preparation protocols, advanced imaging and robust, validated machine-learning segmentation techniques provide first massively multi-scale multi-domain information that enables detailed morphological functional analyses both maternal fetal placental...
The patient was a 26-year-old woman; she developed end stage renal disease (ESRD) due to IgA nephropathy. She on maintenance hemodialysis for one year. Her blood pressure 160/110 mmHg; barely controlled with 4 different anti-hypertensive drugs. It occasionally rose 220/130 mmHg at the of hemodialysis. Fundoscopy revealed Grade Hypertensive Retinopathy and carotid intima media thickness increased (1.0 mm). decreased 140/90 3 days after living donor kidney transplant, requiring monotherapy....
As sample preparation and imaging techniques have expanded improved to include a variety of options for larger sized numbers samples, the bottleneck in volumetric is now data analysis. Annotation segmentation are both common, yet difficult, analysis tasks which required bring meaning data. The SuRVoS application has been updated redesigned provide access manual machine learning-based annotation techniques, including support crowd sourced Combining adjacent, similar voxels (supervoxels)...
Cutaneous T cell lymphoma (CTCL) is a potentially fatal clonal malignancy of cells primarily affecting the skin. The most common form CTCL, mycosis fungoides, can be difficult to diagnose, resulting in treatment delay. We performed single-cell and spatial transcriptomics analysis skin from patients with fungoides-type CTCL an integrated comparative human atlas datasets healthy inflamed revealed co-optation helper 2 (T
When trophoblasts migrate and invade in vivo, they do so by interacting with a range of other cell types, extracellular matrix proteins, chemotactic factors physical forces such as fluid shear stress. These combine to influence overall trophoblast migration invasion into the decidua, which turn determines success spiral artery remodelling, pregnancy itself. Our understanding these important but complex processes is limited simplified conditions we often study vitro, many discrepancies are...
Shoot branching is an essential agronomic trait that impacts on plant architecture and yield. determined by two independent steps: axillary meristem formation bud outgrowth. Although several genes regulatory mechanism have been studied with respect to shoot branching, the roles of chromatin-remodeling factors in developmental process not reported rice. We previously identified a factor OsVIL2 controls trimethylation histone H3 lysine 27 (H3K27me3) at target genes. In this study, we report...
Public participation in research, also known as citizen science, is being increasingly adopted for the analysis of biological volumetric data. Researchers working this domain are applying online science a scalable distributed data approach, with recent research demonstrating that non-experts can productively contribute to tasks such segmentation organelles volume electron microscopy This, alongside growing challenge rapidly process large amounts now routinely produced, means there increasing...
Root hairs are tip-growing cells that emerge from the root epidermis and play a role in water nutrient uptake. One of key signaling steps for polar cell elongation is formation Rho-GTP by accelerating intrinsic exchange activity Rho-of-plant (ROP) or Rac GTPase protein; this step activated through interaction with plant Rho guanine nucleotide factor (RopGEFs). The molecular players involved hair growth rice largely unknown. Here, we performed functional analysis OsRopGEF3 , which highly...
Summary Cereal grain endosperms are an important source of human nutrition. Nuclear division in early endosperm development plays a major role determining seed size; however, this is not well understood. We identified the rice mutant endospermless 2 ( enl2 ), which shows defects stages development. These phenotypes arise from mutations OsCTPS1 that encodes cytidine triphosphate synthase (CTPS). Both wild‐type and were normal at 8 h after pollination (HAP). In contrast, 24 HAP, had...
Photoperiod sensitivity is a dominant determinant for the phase transition in cereal crops. CCT (CONSTANS, CO-like, and TOC1) transcription factors (TFs) are involved many physiological functions including regulation of photoperiodic flowering. However, functional roles TFs have not been elucidated wild progenitors In this study, we identified 41 TFs, 19 CMF, 17 COL, five PRR Oryza rufipogon, presumed ancestor Asian cultivated rice. There thirty-eight orthologous genes sativa, which ten...
In chromatin remodeling, the post-translational modification of histone proteins is mediated by multimeric protein complexes. VERNALIZATION INSENSITIVE3 (VIN3) forms a complex with Polycomb Repressive Complex 2 (PRC2), which mediates trimethylation H3K27 to repress target gene expression. rice, four genes (OsVIL1-OsVIL4) encoding VIN3-like are expressed ubiquitously in various tissues. Null mutants osvil2 display pleiotropic phenotypes such as altered flowering time, floral organ defects,...
Abstract Cutaneous T-cell lymphoma (CTCL) is a potentially fatal clonal malignancy of T cells primarily affecting the skin. The most common form CTCL, mycosis fungoides (MF), can be difficult to diagnose resulting in treatment delay. pathogenesis CTCL not fully understood due limited data from patient studies. We performed single-cell RNA sequencing and spatial transcriptomics profiling skin patients with MF-type an integrated comparative analysis human cell atlas datasets healthy skin,...
Floral transition starts in the leaves when florigens respond to various environmental and developmental factors. Among several regulatory genes that are preferentially expressed inflorescence meristem during floral transition, this study examines homeobox OsZHD1 OsZHD2 for their roles regulating transition. Although single mutations these did not result visible phenotype changes, double delayed flowering. Florigen expression was altered mutants, indicating delay due a defect florigen...
Summary Patients with end-stage kidney disease (ESKD) are at high risk of severe COVID-19. We performed longitudinal single cell multi-omic immune profiling ESKD patients COVID- 19, sampled during two waves the pandemic. Uniquely, for a subset patients, we obtained samples before and acute infection, allowing intra-individual comparison. Using single- transcriptome, surface proteome immunoreceptor sequencing 580,040 high-quality cells, derived from 187 61 demonstrate widespread changes...
Many bioimaging research projects require objects of interest to be identified, located, and then traced allow quantitative measurement. Depending on the complexity system imaging, instance segmentation is often done manually, automated approaches still weeks months an individual’s time acquire necessary training data for AI models. As such, there a strong need develop that minimize use expert annotation while maintaining quality challenging image analysis problems. Herein, we present our...
Summary Human prenatal skin is populated by innate immune cells including macrophages, and whether they act solely in immunity or have additional functions morphogenesis unclear. We assembled the first comprehensive multi-omic reference atlas of human (7-16 post-conception weeks), combining single cell spatial transcriptomic data, to characterise skin’s microenvironmental cellular organisation. This revealed that crosstalk between non-immune underpins formation hair follicles, has...
ABSTRACT Multi-scale structural assessment of biological soft tissue is challenging but essential to gain insight into structure-function relationships tissue/organ. Using the human placenta as an example, this study brings together sophisticated sample preparation protocols, advanced imaging, and robust, validated machine-learning segmentation techniques provide first massively multi-scale multi-domain information that enables detailed morphological functional analyses both maternal fetal...