Ilaria Mulas
A5092451481- Single-cell and spatial transcriptomics
- Congenital heart defects research
- Cell Image Analysis Techniques
- Craniofacial Disorders and Treatments
- Birth, Development, and Health
- Immune Cell Function and Interaction
- dental development and anomalies
- Receptor Mechanisms and Signaling
- Hair Growth and Disorders
- Connective tissue disorders research
- Congenital Heart Disease Studies
- Immunotherapy and Immune Responses
- Cardiovascular Function and Risk Factors
- Dermatology and Skin Diseases
Wellcome Sanger Institute
2023-2025
The function of a cell is defined by its intrinsic characteristics and niche: the tissue microenvironment in which it dwells. Here we combine single-cell spatial transcriptomics data to discover cellular niches within eight regions human heart. We map cells microanatomical locations integrate knowledge-based unsupervised structural annotations. also profile cardiac conduction system
Human prenatal skin is populated by innate immune cells, including macrophages, but whether they act solely in immunity or have additional functions morphogenesis unclear. Here we assembled a comprehensive multi-omics reference atlas of human (7-17 post-conception weeks), combining single-cell and spatial transcriptomics data, to characterize the microanatomical tissue niches skin. This revealed that crosstalk between non-immune cells underpins formation hair follicles, implicated scarless...
Human embryonic bone and joint formation is determined by coordinated differentiation of progenitors in the nascent skeleton. The cell states, epigenetic processes key regulatory factors that underlie lineage commitment these cells remain elusive. Here we applied paired transcriptional profiling approximately 336,000 nucleus droplets spatial transcriptomics to establish a multi-omic atlas human cranium development between 5 11 weeks after conception. Using combined modelling data,...
Abstract Developmental dynamics encompass both the specification of cell types and their spatial organisation into multicellular niches. Here we harness power single-cell multiomics to unravel embryonic foetal cardiac tissue niches, which lead development a new tool, TissueTypist. We reveal that cardiac-resident macrophages likely originate from yolk sac, forming heterogeneous subsets. CX3CR1 + with microglia-like profile localise in sinoatrial node, may contribute axon guidance for...
Abstract The human heart and adjoining great vessels consist of multiple cell types vital for life, yet many remain uncharacterised molecularly during development. Here, we performed a high-resolution profiling the first second trimesters, defining 63 with distinct identity location-specific signatures. We reveal previously unreported types, including pericardium ductus arteriosus. At ventricles, identified signatures involved in establishing trabeculation-compaction right-left axes...
Cancer cells display highly heterogeneous and plastic states in glioblastoma, an incurable brain tumour. However, how these malignant arise whether they follow defined cellular trajectories across tumours is poorly understood. Here, we generated a deep single cell spatial multi-omic atlas of human glioblastoma that pairs transcriptomic, epigenomic genomic profiling 12 multiple regions. We identify heterogeneity driven by spatially-patterned transitions cancer from developmental-like towards...
Abstract Bone and joint formation in the developing skeleton rely on co-ordinated differentiation of progenitors nascent limbs joints. The cell states, epigenetic processes key regulatory factors underlying their lineage commitment to osteogenic other mesenchymal populations during ossification remain poorly understood are largely unexplored human studies. Here, we apply paired single-nuclei transcriptional profiling 336,000 droplets, addition spatial transcriptomics, construct a...
Summary Human prenatal skin is populated by innate immune cells including macrophages, and whether they act solely in immunity or have additional functions morphogenesis unclear. We assembled the first comprehensive multi-omic reference atlas of human (7-16 post-conception weeks), combining single cell spatial transcriptomic data, to characterise skin’s microenvironmental cellular organisation. This revealed that crosstalk between non-immune underpins formation hair follicles, has...