A5006091218- Melanoma and MAPK Pathways
- Computational Drug Discovery Methods
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- Advanced Biosensing Techniques and Applications
- RNA regulation and disease
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Cutaneous Melanoma Detection and Management
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Cutaneous lymphoproliferative disorders research
- Cancer Genomics and Diagnostics
- Nonmelanoma Skin Cancer Studies
- Histone Deacetylase Inhibitors Research
- HER2/EGFR in Cancer Research
- S100 Proteins and Annexins
- melanin and skin pigmentation
- MicroRNA in disease regulation
- bioluminescence and chemiluminescence research
- Psoriasis: Treatment and Pathogenesis
Medical University of Vienna
2018-2025
FH Campus Wien
2023-2024
Abstract Immune cells need to sustain a state of constant alertness over lifetime. Yet, little is known about the regulatory processes that control fluent and fragile balance called homeostasis. Here we demonstrate JAK-STAT signaling, beyond its role in immune responses, major regulator cell We investigated JAK-STAT-mediated transcription chromatin accessibility across 12 mouse models, including knockouts all STAT factors TYK2 kinase. Baseline signaling was detected CD8 + T macrophages...
We present a single-arm, phase II, neoadjuvant trial with the oncolytic virus talimogene laherparepvec (T-VEC) in 18 patients difficult-to-resect cutaneous basal cell carcinomas. The primary end point, defined as proportion of patients, who after six cycles T-VEC (13 weeks), become resectable without need for plastic reconstructive surgery, was already achieved stage I (9 patients; 50.0%); thus study discontinued early success. objective response rate 55.6% and complete pathological 33.3%....
DNA methylation profiles have emerged as potential predictors of therapeutic response in various solid tumors. This study aimed to analyze the patients with stage IV metastatic melanoma undergoing first-line immune checkpoint inhibitor treatment and evaluate their correlation a radiological according immune-related Response Evaluation Criteria Solid Tumors (iRECIST). A total 81 tissue samples from 71 (27 female, 44 male) were included this study. We utilized Illumina Methylation EPIC...
Abstract Clinical management of melanomas with NRAS mutations is challenging. Targeting MAPK signaling only beneficial to a small subset patients due resistance that arises through genetic, transcriptional, and metabolic adaptation. Identification targetable vulnerabilities in NRAS-mutated melanoma could help improve patient treatment. Here, we used multiomics analyses reveal cells adopt mesenchymal phenotype quiescent program resist cellular stress induced by MEK inhibition. The alterations...
Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT contributing to tissue regeneration are not yet completely clear. In this study, we have superficial model tape stripping of ex vivo human skin. Protein profiles wounded biopsies treated OCT-containing hydrogel and released secretome were analyzed using liquid chromatography-mass spectrometry...
Abstract Purpose: Despite high clinical need, there are no biomarkers that accurately predict the response of patients with metastatic melanoma to anti-PD-1 therapy. Experimental Design: In this multicenter study, we applied protein depletion and enrichment methods prior various proteomic techniques analyze a serum discovery cohort (n = 56) three independent validation cohorts 80, n 12, 17). Further analyses by literature survival analysis followed. Results: We identified several...
Targeted therapies against mutant BRAF are effectively used in combination with MEK inhibitors (MEKi) to treat advanced melanoma. However, treatment success is affected by resistance and adverse events (AEs). Approved (BRAFi) show high levels of target promiscuity, which can contribute these effects. The blood vessel lining direct contact plasma concentrations BRAFi, but effects the this cell type unknown. Hence, we aimed characterize responses approved BRAFi for melanoma vascular...
Abstract Aims Extracorporeal photopheresis (ECP) is a UVA-based phototherapy of whole blood and well established as first line or combination therapy for the treatment cutaneous T-cell lymphoma, systemic sclerosis, graft-versus-host disease used to control organ transplant rejection. While proapoptotic activity on activated T-cells evident, clinical efficacy this also appears be based other yet unknown mechanisms. In study, we aimed identify novel mechanisms ECP regardless patient’s...
Cutaneous T cell lymphoma (CTCL) is a potentially fatal clonal malignancy of cells primarily affecting the skin. The most common form CTCL, mycosis fungoides, can be difficult to diagnose, resulting in treatment delay. We performed single-cell and spatial transcriptomics analysis skin from patients with fungoides-type CTCL an integrated comparative human atlas datasets healthy inflamed revealed co-optation helper 2 (T
MAP kinase inhibitor (MAPKi) therapy for BRAF mutated melanoma is characterized by high response rates but development of drug resistance within a median progression-free survival (PFS) 9–12 months. Understanding mechanisms and identifying effective therapeutic alternatives one the most important scientific challenges in melanoma. Using proteomics, we want to specifically gain insight into pathophysiological process cerebral metastases. Cerebral metastases from patients were initially...
Abstract Psoriasis is a common chronic inflammatory skin disease characterized by thickened epidermis with elongated rete ridges and massive immune cell infiltration. It currently unclear what impact mechanoregulatory aspects in the dermis may have on progression. Using multiphoton second harmonic generation microscopy we found that extracellular matrix (ECM) was profoundly reorganized within psoriasis compared to healthy skin. Collagen fibers were highly aligned assembled into thick, long...
Psoriasis is a common chronic inflammatory skin disease characterized by thickened epidermis with elongated rete ridges and massive immune cell infiltration. It currently unclear what impact mechanoregulatory aspects may have on progression. Using multiphoton second harmonic generation microscopy we found that the extracellular matrix (ECM) was profoundly reorganized within psoriatic dermis. Collagen fibers were highly aligned assembled into thick, long collagen bundles, whereas overall...
The implications of infiltrative compared to non-infiltrative growth cutaneous basal cell carcinoma (BCC) on the tumor stroma and immune landscape are unknown. This is clinical importance, because BCCs, in contrast other BCC subtypes, more likely relapse after surgery radiotherapy.This descriptive cross-sectional study analyzed 38 BCCs collected from 2018 2021. In first cohort (n = 28), cells were characterized by immunohistochemistry multiplex immunofluorescence staining for CD3, CD8, CD68,...
Abstract Cutaneous T-cell lymphoma (CTCL) is a potentially fatal clonal malignancy of T cells primarily affecting the skin. The most common form CTCL, mycosis fungoides (MF), can be difficult to diagnose resulting in treatment delay. pathogenesis CTCL not fully understood due limited data from patient studies. We performed single-cell RNA sequencing and spatial transcriptomics profiling skin patients with MF-type an integrated comparative analysis human cell atlas datasets healthy skin,...
Summary In malignant diseases, targeting of immune checkpoints successfully changed the therapeutic landscape and helped to unleash anti-tumor T cell responses, resulting in durable clinical outcomes, but only up 50% patients. The success these therapies need overcome intrinsic acquired therapy resistance stimulated research identify new pathways targets. Numerous trials are currently evaluating novel checkpoint inhibitors or recently developed strategies like modulating tumor...
9561 Background: Immune checkpoint inhibitors (ICIs) have revolutionized the treatment of metastatic melanoma with objective response rates 40-58%. However, reliable biomarkers to predict are lacking. Tumor tissue methylation profiles were recently proposed predictive value in various solid organ tumor entities. Methods: Patients melanoma, American Joint Committee on Cancer (AJCC; 8 th Edition) stage IV, receiving first-line ICI-based therapy, retrospectively identified and formalin-fixed...
<p>Differentially expressed proteins (DEPs) and functional annotation of NR proteomes based on GO term classification.</p>
<p>Primary melanoma tumor cells of NR display a distinctive immunophenotype.</p>