A5087441729- Polyamine Metabolism and Applications
- GABA and Rice Research
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Amino Acid Enzymes and Metabolism
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Food Quality and Safety Studies
- Plant responses to water stress
- Chromosomal and Genetic Variations
- Aldose Reductase and Taurine
- Light effects on plants
- CRISPR and Genetic Engineering
- Sphingolipid Metabolism and Signaling
- Enzyme-mediated dye degradation
- Transgenic Plants and Applications
- Biochemical and biochemical processes
- Nematode management and characterization studies
- Phytochemicals and Antioxidant Activities
Sunchon National University
2013-2024
Korea Basic Science Institute
2012
Pohang University of Science and Technology
2003-2008
Seoul National University
2003-2004
Gachon University
2004
MizMedi Hospital
2004
University of California, Berkeley
2004
Yonsei University
1991-2003
Pennsylvania State University
2003
University of Suwon
2003
Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of pluripotent embryonic stem (ES) line (SCNT-hES-1) from cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES morphology and surface markers were capable differentiating into embryoid bodies in vitro forming teratomas vivo containing derivatives all three germ layers severe combined immunodeficient mice. After...
Abstract We analyzed 6,749 lines tagged by the gene trap vector pGA2707. This resulted in isolation of 3,793 genomic sequences flanking T-DNA. Among insertions, 1,846 T-DNAs were integrated into genic regions, and 1,864 located intergenic regions. Frequencies also higher at beginning end coding regions upstream near ATG start codon. The overall GC content insertion sites was close to that measured from entire rice (Oryza sativa) genome. Functional classification these genes showed a...
We have examined the expression of mRNAs for S-adenosylmethionine synthetase (EC 2.5.1.6), 1-aminocyclopropane-1-carboxylate (ACC) synthase 4.4.1.14), and ethylene-forming enzyme (EFE) in various floral organs carnation (Dianthus caryophyllus) during increase ethylene biosynthesis associated with petal senescence. The abundance ACC EFE increased transcripts decreased concomitantly climacteric senescing petals. aging flowers was prevented by treatment action inhibitor 2,5-norbornadiene....
Reactive oxygen species (ROS), such as H2O2, are important plant cell signaling molecules involved in responses to biotic and abiotic stresses developmental physiological processes. Despite the well-known functions of ethylene production stress via ROS during stresses, whether acts alone or conjunction with has not yet been fully elucidated. Therefore, we investigated relationship between accumulation response stress. We used three independent transgenic tobacco lines, CAS-AS-2, −3 −4, which...
We observed the biphasic production of ethylene and reactive oxygen species (ROS) in susceptible tobacco (Nicotiana tabacum 'Wisconsin 38') plants after shoot inoculation with Phytophthora parasitica var nicotianae. The initial transient increase ROS at 1 3 h (phase I), respectively, was followed by a second massive 48 72 II), pathogen inoculation. This pattern significantly differed from hypersensitive response exhibited cryptogein-treated wild-type plants. mediated both NADPH oxidase...
The apoplastic polyamine oxidase (PAO) catalyzes the oxidation of higher polyamines spermidine and spermine, contributing to hydrogen peroxide (H2O2) accumulation. However, it is yet unclear whether PAO part a network that coordinates accumulation reactive oxygen species (ROS) under salinity or if acts independently. Here, we unravel NADPH cooperate control H2O2 superoxides (O2·-) in tobacco (Nicotiana tabacum). To examine what extent constitutes ROS-generating network, examined ROS guard...
Abstract Plant MADS-box genes can be divided into 11 groups. Genetic analysis has revealed that most of them function in flowering-time control, reproductive organ development, and vegetative growth. Here, we elucidated the role OsMADS26, a member AGL12 group. Transcript levels OsMADS26 were increased an age-dependent manner shoots roots. Transgenic plants both rice (Oryza sativa) Arabidopsis (Arabidopsis thaliana) overexpressing this gene manifested phenotypes related to stress responses,...
The diamine putrescine and the polyamines (PAs), spermidine (Spd) spermine (Spm), are ubiquitously occurring polycations associated with several important cellular functions, especially anti-senescence. Numerous studies have reported increased levels of polyamine in plant cells under conditions abiotic biotic stress such as drought, high salt concentrations, pathogen attack. However, physiological mechanism elevated response to stresses remains undetermined. Transgenic plants having...
Rae1 performs multiple functions in animal systems, acting interphase as an mRNA export factor and during mitosis a mitotic checkpoint spindle assembly regulator. In this study we characterized of plants. Virus-induced gene silencing Nicotiana benthamiana Rae1, NbRae1, which encodes protein with four WD40 repeats, resulted growth arrest abnormal leaf development. NbRae1 was mainly associated the nuclear envelope interphase, deficiency caused accumulation poly(A) RNA nuclei cells, suggesting...
A highly oxidative stress-tolerant japonica rice line was isolated by T-DNA insertion mutation followed screening in the presence of 50 mM H2O2. The mapped to locus Os09g0547500, gene product which annotated as lysine decarboxylase-like protein (GenBank accession No. AK062595). We termed this OsLDC-like 1, for Oryza sativa 1. site second exon and resulted a 27 amino acid N-terminal deletion. Despite defect mutant exhibited enhanced accumulation polyamines (PAs) putrescine, spermidine,...
Salt stress causes rapid accumulation of nonexpressor pathogenesis-related genes 1 (NPR1) protein, known as the redox-sensitive transcription coactivator, which in turn elicits many adaptive responses. The NPR1 protein transiently accumulates chloroplast stroma under salt stress, attenuates stress-triggered down-regulation photosynthetic capability. We observed that oligomeric chloroplasts and cytoplasm had chaperone activity, whereas monomeric nucleus did not. Additionally, overexpression...
Root meristem activity is the most critical process influencing root development. Although several factors that regulate have been identified in rice, studies on enhancement of roots are limited. We a T-DNA activation tagging line zinc-finger homeobox gene, OsZHD2, which has longer seminal and lateral due to increased activity. The phenotypes were confirmed transgenic plants overexpressing OsZHD2. In addition, showed enhanced grain yield under low nutrient paddy field conditions. OsZHD2 was...
The amount of polyamines (such as putrescine, spermidine, and spermine) increased under environmental stress conditions. We used transgenic technology in an attempt to evaluate their potential for mitigating the adverse effects several abiotic stresses plants. Because there is a metabolic competition S-adenosylmethionine precursor between polyamine (PA) ethylene biosyntheses, it was expected that antisense-expression biosynthetic genes could result increase PA biosynthesis. Antisense...
A biphasic reactive oxygen species (ROS) production has previously been observed in tobacco at 1 and 48 h after inoculation with the hemibiotrophic compatible pathogen, Phytophthora parasitica var. nicotianae (Ppn). To characterize response of to biphasically produced ROS concerning propagation Ppn, ultraperformance liquid chromatography-quadrupole-time flight/ mass spectrometry (UPLC-Q-TOF/MS) based metabolic profiling combined multivariate statistical analysis was performed. Among...
Summary Arginine decarboxylase (ADC; EC 4.1.1.9) is a key enzyme in polyamine biosynthesis plants. We characterized carnation genomic clone, gDcADC8 , which the deduced polypeptide of ADC was 725 amino acids with molecular mass 77.7 kDa. The unusually long 5′‐UTR that contained short upstream open reading frame (uORF) seven (MQKSLHI) predicted to form an extensive secondary structure (free energy approximately −117 kcal mol −1 ) using Zuker m‐fold algorithm. result antibody detected two...
Brassinosteroid (BR), an endogenous steroid growth regulator of higher plants, enhances expansion and division the cell in a number plant species. It has been recently reported that shared auxin–BR signalling pathway is involved seedling Arabidopsis . Here, we show BR specifically enhanced expression AtACS4 , which encodes auxin‐responsive ACC synthase 4, by distinct temporal induction mechanism compared with IAA etiolated seedlings. This was undetectable light‐grown In addition, failed to...