A5101978030- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Microplastics and Plastic Pollution
- biodegradable polymer synthesis and properties
- Bone Tissue Engineering Materials
- Lung Cancer Treatments and Mutations
- Dental Implant Techniques and Outcomes
- Reproductive Biology and Fertility
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Tissue Engineering and Regenerative Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Renal and related cancers
- Intensive Care Unit Cognitive Disorders
- Tracheal and airway disorders
- Animal Genetics and Reproduction
- Additive Manufacturing and 3D Printing Technologies
- Respiratory Support and Mechanisms
- Phonocardiography and Auscultation Techniques
- RNA modifications and cancer
- Carbon dioxide utilization in catalysis
- Ultrasound in Clinical Applications
- Streptococcal Infections and Treatments
- Periodontal Regeneration and Treatments
- Respiratory and Cough-Related Research
Ewha Womans University Medical Center
2023
CJ CheilJedang (South Korea)
2022
Yonsei University
2020-2022
Konkuk University
2010-2020
Konkuk University Medical Center
2005-2020
Ewha Womans University
2013
Kumoh National Institute of Technology
2013
MizMedi Hospital
2001-2006
Hanyang University
1999-2006
Seoul National University
2004-2005
Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of pluripotent embryonic stem (ES) line (SCNT-hES-1) from cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES morphology and surface markers were capable differentiating into embryoid bodies in vitro forming teratomas vivo containing derivatives all three germ layers severe combined immunodeficient mice. After...
Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies disease and development advance clinical deliberations regarding cell transplantation. Eleven hESC lines were established by somatic nuclear transfer (SCNT) skin from patients with or injury into donated oocytes. These lines, (NT)-hESCs, grown on feeders the same NT donor genetically unrelated individuals, at high rates, regardless sex age. NT-hESCs...
Human embryonic stem (hES) cells are usually established and maintained on mouse fibroblast (MEFs) feeder layers. However, it is desirable to develop human because animal associated with risks such as viral infection and/or pathogen transmission. In this study, we attempted establish new hES cell lines using uterine endometrial (hUECs) prevent the for their eventual application in cell-replacement therapy. Inner masses (ICMs) of cultured blastocysts were isolated by immunosurgery then...
Human embryonic stem (hES) cells have been traditionally cultured on primary mouse fibroblasts (PMEFs). However, though STO some advantages over PMEFs and human (hEFs) as feeder cells, they never used to establish hES cell lines. In this study, three lines (Miz-hES1, Miz-hES2, Miz-hES3) were established from inner masses (ICM), using cells. The had normal karyotypes expressed high levels of alkaline phosphatase (AP), surface markers (SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), transcription factor...
Mouse embryonic fibroblasts (MEFs) have been previously used as feeder cells to support the growth of human stem (hESCs). In this study, adult uterine endometrial (hUECs), breast parenchymal (hBPCs) and (hEFs) were tested for supporting hESCs prevent possibility contamination from animal cells. Cultured hUECs, hBPCs hEFs mitotically inactivated then plated. (Miz-hES1, NIH registered) initially established on mouse layers transferred onto each layer split every 5 days. The morphology,...
Reason: upon re‐examination of their work, the authors have found that data in Figure 2A was intentionally fabricated (the blots 3rd and 4th rows are duplicated mirror images). As a result, elected to withdraw complete article with apologies scientific community.
In this study, poly(butylene sebacate-co-terephthalate) (PBSeT) was successfully synthesized using various ratios of sebacic acid (Se) and dimethyl terephthalate (DMT). The PBSeT showed a high molecular weight (Mw, 88,700–154,900 g/mol) good elastomeric properties. particular, the PBSeT64 (6:4 acid/dimethyl mole ratio) sample an elongation at break value over 1600%. However, further increasing DMT content decreased properties but increased tensile strength due to inherent aromatic unit....
Poly(butylene sebacate-co-terephthalate) (PBSeT) is a biodegradable flexible polymer suitable for melt blending with other polymers. Melt compatibilizer common strategy increasing miscibility between In this study, PBSeT polyester was synthesized, and poly(lactic acid) (PLA) blended 25 wt% by processing 3-6 phr PLA-grafted maleic anhydride (PLA-g-MAH) compatibilizers. PLA-g-MAH enhanced the interfacial adhesion of PLA/PBSeT blend, their mechanical morphological properties confirmed that also...
Human embryonic stem cells (hESCs) have the potential to differentiate into various cell types, and three germ layers in vivo vitro. They are therefore useful transplantation tissue engineering. Here, we describe expression patterns of selected steroid receptor mRNAs - estrogen receptor-α (ER-α), ER-β, glucocorticoid (GR), progesterone (PR) undifferentiated hESCs embryoid bodies (EBs) cultured for 2, 4, 6 d, as assessed by real-time PCR, order define possible influence hormones on...
Direct sequencing is the standard method for detection of epidermal growth factor receptor (EGFR) mutations in lung cancer, however, its relatively low sensitivity limits clinical use. Pyrosequencing a bioluminometric, real-time non-electrophoretic DNA technique with number advantages compared direct sequencing, including higher sensitivity, speed, automation and cost-effectiveness. Clinical specimens from 202 cancer patients were analyzed EGFR exons 18, 19, 20 21 using pyrosequencing...
Abstract Background Immunoglobulin G4-related lung disease (IgG4-RLD) is the pulmonary manifestation of a systemic fibroinflammatory characterized by lymphoplasmacytic infiltration with an abundance IgG4-positive plasma cells. Long-term clinical course and outcomes IgG4-RLD remain unclear. We aimed to identify characteristics, treatment outcomes, longitudinal function changes in patients according radiologic classification. Methods Chest computed tomography findings 37 subjects were...
Abstract Background Alopecia refers to a condition developed by gradual reduction of hair loss various abnormal causes such as endocrine system, genetic factors, and stress. Stromal vascular fraction (SVF) isolated from the fat is one latest innovative solutions in field regeneration therapy. We focused on presenting effectiveness clinical cases improve AGA through transplantation autologous SVF into scalp. Objective To confirm efficacy usage patients with AGA. Methods Nine (age range 43–64...
Poly (lactic acid) (PLA) is the most widely available commercial bioplastic that used in various medical and packaging applications three-dimensional filaments. However, because neat PLA brittle, it conventionally has been blended with ductile polymers plasticizers. In this study, was high-ductility biopolymer poly (butylene-sebacate–co–terephthalate) (PBSeT), hexamethylene diisocyanate (HDI) applied as a crosslinking compatibilizer to increase miscibility between two polymers. (80%) PBSeT...
Abstract Melatonin, secreted by the pineal gland, is involved in regulation of many physiological functions various species animals. In present study, expression gene for melatonin Mel 1a receptor (MelR) was evaluated ovary, hypothalamus, and pituitary according to developmental stages female mice. Semiquantitative reverse‐transcription polymerase chain reaction (RT‐PCR) situ PCR techniques were applied. According stages, MelR differently expressed on pituitary. first prior hypothalamus...
Previous reports have indicated that extracellular matrices (ECMs) affect the developmental fate of human embryonic stem cells (hESCs). Specially, type IV collagen and laminin, which belong to a group macromolecular proteins with substantial proportion ECMs, are known influence proliferation differentiation hES cells. In this study, we evaluated effects laminin in freezing medium on survival rates after slow rapid thawing. The addition (1 μg/ml) significantly increased rate thawing compared...
Human embryonic stem (ES) cells and germ (EG) are pluripotent invaluable material for in vitro studies of human embryogenesis cell therapy. So far, only two groups have reported the establishment EG lines, whereas at least five ES lines been established. To see if can be reproducibly established, we isolated primordial (PGCs) from gonadal ridges mesenteries (9 weeks postfertilization) cultured them on mouse STO cells. As with colonies, PGC-derived given rise to multilayered colonies without...
Human embryonic stem (hES) cells, unlike most cells derived from adult or fetal human tissues, represent a potentially unlimited source of various cell types for basic clinical research. To meet the increased demand characterized hES lines, we established and nine new lines obtained frozen-thawed pronucleus-stage embryos. In addition, improved derivation efficiency inner masses (to 47.4%) optimized culture conditions undifferentiated cells. After these had been maintained over year in vitro,...
This research aimed to investigate the changes in ethical issues everyday clinical practice recognized by critical care nurses during two observation periods. We conducted a retrospective analysis of data obtained prospective questionnaire surveys intensive units (ICU) tertiary university-affiliated hospital Seoul, Korea. Data were collected prospectively different periods, February 2002-January 2003 (Period 1) and August 2011-July 2012 2). Significantly fewer cases with reported Period 2...