A5100678328- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- Molecular Sensors and Ion Detection
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Marine Ecology and Invasive Species
- interferon and immune responses
- Enzyme Production and Characterization
- Cancer-related Molecular Pathways
- Proteins in Food Systems
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Bioactive Compounds and Antitumor Agents
- NF-κB Signaling Pathways
- Cancer Mechanisms and Therapy
- 3D Printing in Biomedical Research
- Retinoids in leukemia and cellular processes
Gangneung–Wonju National University
2021-2024
Korea University Medical Center
2024
Chung-Ang University
2023-2024
Kyungpook National University
2016-2024
Korea University
2024
Seoul National University Bundang Hospital
2018
Seoul National University
2014-2018
National Cancer Center
2011-2017
California State University, Long Beach
2017
Government of the Republic of Korea
2017
Although Argonaute (AGO) proteins have been the focus of microRNA (miRNA) studies, we observed AGO-free mature miRNAs directly interacting with RNA-binding proteins, implying sophisticated nature fine-tuning gene regulation by miRNAs. To investigate microRNA-binding (miRBPs) globally, analyzed PAR-CLIP data sets to identify RBP quaking (QKI) as a novel miRBP for let-7b. Potential existence were further verified measuring miRNA levels in genetically engineered AGO-depleted human and mouse...
Four imidazolium-based ionic liquids (ILs) with two cations 1-pentyl-3-butylimidazolium [PBIM]+ and 1-benzyl-3-butylimidazolium tetrafluoroborate [BzBIM]+, anions (BF4−) trifluoromethanesulfonate (OTf−) were synthesized for NH3 solubility enhancement. The structural, thermal, electrochemical stabilities, conductivity, viscosity of the four ILs, namely, [PBIM]BF4, [BzBIM]BF4, [PBIM]OTf, [BzBIM]OTf, investigated. Due to intermolecular interaction benzyl group attached imidazolium ring,...
Human embryonic stem cells (hESCs) have the potential to differentiate into various cell types, and three germ layers in vivo vitro. They are therefore useful transplantation tissue engineering. Here, we describe expression patterns of selected steroid receptor mRNAs - estrogen receptor-α (ER-α), ER-β, glucocorticoid (GR), progesterone (PR) undifferentiated hESCs embryoid bodies (EBs) cultured for 2, 4, 6 d, as assessed by real-time PCR, order define possible influence hormones on...
Abstract Inflammatory cytokines are key signaling molecules that can promote an immune response, thus their RNA turnover must be tightly controlled during infection. Most studies investigate the decay pathways in cytosol or nucleoplasm but never focused on nucleolus. Although this organelle has well-studied roles ribosome biogenesis and cellular stress sensing, mechanism of within nucleolus is not completely understood. Here, we report essential site inflammatory pre-mRNA instability...
Melanogenesis can be controlled by tyrosinase inhibition or blocking the maturation processes of and its related proteins. Mangostenone F was isolated from seedcases Garcinia mangostana. shown to inactive against (IC50 > 200 μM) but a potent α-glucosidase inhibitor in vitro = 21.0 μM). found inhibit production melanin mouse melanoma cell line B16F10. Importantly, unlike most glycosidase inhibitors, mangostenone displayed very low cytotoxicity (EC50 The Western blot for expression levels...
Post-transcriptional gene regulation is an important step in the of eukaryotic expression. Subcellular compartmentalization RNA species plays a crucial role control mRNA turnover, spatial restriction protein synthesis, and formation macromolecular complexes. Although long noncoding RNAs (lncRNAs) are one key regulators post-transcriptional expression, it not heavily studied whether localization lncRNAs subcellular organelles has functional consequences. Here, we report on mitochondrial whose...
Argonaute (AGO), a component of RNA-induced silencing complexes (RISCs), is representative RNA-binding protein (RBP) known to bind with mature microRNA (miRNA) and directly involved in post-transcriptional gene silencing. However, despite the biological significance miRNA, roles other micro proteins (miRBPs) remain unclear regulation miRNA loading, dissociation from RISC, extracellular release. In this study, we perform arrays profile miRBPs identify 118 binding miRNAs. Among those proteins,...
The combination of ifosfamide and etoposide (IE) is commonly used to treat relapsed or refractory osteosarcoma; however, second-line treatment recommendations vary across guidelines.
Somatic amplifications of the LYL1 gene are relatively common occurrences in patients who develop uterine corpus endometrial carcinoma (UCEC) as opposed to other cancers. This study was undertaken determine whether such genetic alterations affect survival outcomes UCEC. In 370 with UCEC, we analysed clinicopathologic characteristics and corresponding genomic data from The Cancer Genome Atlas database. Patients were stratified according status, grouped amplification or non-amplification....
The tubby protein (Tub), a putative transcription factor, plays important roles in the maintenance and function of neuronal cells. A splicing defect-causing mutation 3'-end gene, which is predicted to disrupt carboxy-terminal region Tub protein, causes maturity-onset obesity, blindness, deafness mice. Although this pathological leads loss function, precise mechanism has not yet been investigated. Here, we found that mutant proteins were mostly localized puncta perinuclear C-terminus was for...
pH-Responsive oligodeoxynucleotides only exhibited dramatic increases in fluorescence upon duplex formation with their fully matched target DNAs.
Massive all-atom molecular dynamics simulations were conducted across a distributed computing network to study the folding, unfolding, misfolding and conformational plasticity of high-efficiency frameshifting double mutant 26 nt potato leaf roll virus RNA pseudoknot. Our robust sampling, which included over 40 starting structures spanning spectrum from extended unfolded state native fold, yielded nearly 120 μs cumulative sampling time. Conformational microstate transitions on 1.0 ns 10.0...
Formalin-fixed paraffin-embedded (FFPE) tissues are utilized as the standard diagnostic method in pathology laboratories. However, admixture of unwanted and shortage normal samples, which can be used to detect somatic mutation, considered critical factors accurately diagnose cancer. To explore these challenges, we sorted pure tumor cells from 22 FFPE lung adenocarcinoma via Di-Electro-Phoretic Array (DEPArray) technology, a new cell sorting analyzed variants with next-generation sequencing...
<title>Abstract</title> <bold>Background</bold> Breast Cancer (BC) is a complex disease with profound genomic aberrations. However, the underlying molecular disparity influenced by age and ethnicity remains elusive. <bold>Methods</bold> In this study, we aimed to investigate properties of 843 primary metastatic BC patients enrolled in K-MASTER program. By categorizing into two distinct subgroups, explored their unique properties. Additionally, leveraged large-scale data from TCGA MSK-IMPACT...
Global warming caused from the heavy consumption of fossil fuel is one biggest problems to be solved. Biofuel has been gained more attention as an alternative reduce fuel. Recently, butanol produced genus Clostridium considered promising alternatives for gasoline, based Nevertheless, lack genome-engineering tools major hurdle economic production butanol. More recently, genome engineering have developed metabolic butanol-producing species, which includes scale network model and editing on...