A5086039126- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cellular Mechanics and Interactions
- Galectins and Cancer Biology
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Helicobacter pylori-related gastroenterology studies
- Advanced Fluorescence Microscopy Techniques
- Kruppel-like factors research
- Coral and Marine Ecosystems Studies
- Cancer Genomics and Diagnostics
- Hippo pathway signaling and YAP/TAZ
- Immune Response and Inflammation
- Wnt/β-catenin signaling in development and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Neuroinflammation and Neurodegeneration Mechanisms
- Aquaculture disease management and microbiota
- Cell Adhesion Molecules Research
- RNA regulation and disease
- Chromosomal and Genetic Variations
- Nuclear Structure and Function
Natera (United States)
2025
Medical University of South Carolina
2015-2024
College of Charleston
2014-2019
PURPOSE This study aimed to assess (1) the prognostic value of circulating tumor DNA (ctDNA) and (2) ability ctDNA detect recurrence compared with standard surveillance in curatively resected early-stage biliary tract cancer (BTC). METHODS retrospective, multicenter cohort evaluated serial testing for patients BTC after curative resection. We relapse-free survival (RFS) by positivity. The sensitivity detecting a confirmed BTC, defined as biopsy-proven or true progression radiographic...
Hearing relies on the transmission of auditory information from sensory hair cells (HCs) to brain through nerve. This relay requires HCs be innervated by spiral ganglion neurons (SGNs) in an exclusive manner and SGNs ensheathed myelinating non-myelinating glial cells. In developing nerve, mistargeted SGN axons are retracted or pruned excessive cleared a process referred as nerve refinement. Whether eliminated during refinement is unknown. Using early postnatal mice either sex, we show that...
The RNA interference (RNAi) machinery is an essential component of the cell, regulating miRNA biogenesis and function. RNAi complexes were thought to localize either in nucleus, such as microprocessor, or cytoplasm, RNA-induced silencing complex (RISC). We recently revealed that core microprocessor components DROSHA DGCR8, well main RISC, including Ago2, also associate with apical adherens junctions well-differentiated cultured epithelial cells. Here, we demonstrate localization specific...
Abstract Epithelial adherens junctions (AJs) are cell-cell adhesion complexes that influenced by tissue mechanics, such as those emanating from the extracellular matrix (ECM). Here, we introduce a mechanism whereby epithelial AJs can also regulate ECM. We show AJ component PLEKHA7 regulates levels and activity of key ECM remodeling components MMP1 LOX in well-differentiated colon cells, through miR-24 miR-30c miRNAs. depletion cells results LOX-dependent culture colonic mucosal lamina...
A key difference between the Tourist and Stowaway families of miniature inverted repeat transposable elements (MITEs) is manner in which their excision alters genome. Upon excision, Stowaway-like MITEs associated Mariner usually leave behind a small duplication short sequences from end element. These insertions or deletions known as "footprints" can potentially disrupt coding regulatory sequences. In contrast, Tourist-like PIF/Pong/Harbinger generally excise precisely, returning genome to...
Adherens junctions are cadherin-based structures critical for cellular architecture. E-cadherin in mature epithelial cell monolayers tether to an apical actomyosin ring form the zonula adherens (ZA). We have previously shown that junction protein PLEKHA7 associates with and regulates function of core RNA interference (RNAi) component AGO2 specifically at ZA. However, mechanism mediating recruitment ZA remained unexplored. Here, we reveal this ZA-specific depends on both structural tensile...
ABSTRACT Adherens junctions are cadherin-based structures critical for cellular architecture. E-cadherin in mature epithelial cell monolayers tether to an apical actomyosin ring form the zonula adherens (ZA). We have previously shown that junction protein PLEKHA7 associates with and regulates function of core RNA interference (RNAi) component AGO2 specifically at ZA. However, mechanism mediating Ago2 recruitment ZA remained unexplored. Here, we reveal this ZA-specific depends on both...
OBJECTIVES/SPECIFIC AIMS: The goal of this study is to test the hypothesis that adherens junctions colon epithelial cells regulate lncRNAs levels and function via microprocessor RISC complexes suppress expression pro-tumorigenic markers aberrant cell behavior. METHODS/STUDY POPULATION: To hypothesis, we used cancer lines. We performed RNA-seq following knockdown PLEKHA7, a key component junctions, identify changes in lncRNA downstream mRNA levels. confirmed junctional localization affected...
The adherens junctions (AJs) are essential architectural elements of epithelial tissues. Compromised junctional integrity is a common precursor to colon cancer. Recently, we identified novel mechanism whereby the AJs non-transformed cells recruit microprocessor and RISC, core RNAi machinery, as well miRNAs, suppress oncogenic mRNAs. Knockdown AJ component PLEKHA7, disrupts this RNAi-mediated signaling program, leading pro-tumorigenic cell transformation. Interestingly, PLEKHA7 RNA-CLIP...
Abstract The adherens junctions (AJs) are essential architectural elements of epithelial tissues. Compromised junctional integrity is a common precursor to colon cancer. Recently, we identified novel mechanism whereby the AJs non-transformed cells recruit microprocessor and RISC, core RNAi machinery, as well miRNAs, suppress oncogenic mRNAs. Knockdown AJ component PLEKHA7, disrupts this RNAi-mediated signaling program, leading pro-tumorigenic cell transformation. Interestingly, PLEKHA7...