A5051210100- RNA Research and Splicing
- Cellular Mechanics and Interactions
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Magnesium Alloys: Properties and Applications
- Wnt/β-catenin signaling in development and cancer
- Kruppel-like factors research
- Cancer-related gene regulation
- Tendon Structure and Treatment
Medical University of South Carolina
2019-2024
The RNA interference (RNAi) machinery is an essential component of the cell, regulating miRNA biogenesis and function. RNAi complexes were thought to localize either in nucleus, such as microprocessor, or cytoplasm, RNA-induced silencing complex (RISC). We recently revealed that core microprocessor components DROSHA DGCR8, well main RISC, including Ago2, also associate with apical adherens junctions well-differentiated cultured epithelial cells. Here, we demonstrate localization specific...
Abstract Epithelial adherens junctions (AJs) are cell-cell adhesion complexes that influenced by tissue mechanics, such as those emanating from the extracellular matrix (ECM). Here, we introduce a mechanism whereby epithelial AJs can also regulate ECM. We show AJ component PLEKHA7 regulates levels and activity of key ECM remodeling components MMP1 LOX in well-differentiated colon cells, through miR-24 miR-30c miRNAs. depletion cells results LOX-dependent culture colonic mucosal lamina...
The extracellular matrix (ECM) plays crucial roles in tissue homeostasis. Abnormalities ECM composition are associated with pathological conditions, such as fibrosis and cancer. These alterations sensed by the epithelium can influence its behavior through crosstalk other mechanosensitive complexes, including adherens junctions (AJs). We have previously shown that AJs, their component PLEKHA7, recruit RNAi machinery to regulate miRNA levels function. particularly junctional localization of...
Adherens junctions are cadherin-based structures critical for cellular architecture. E-cadherin in mature epithelial cell monolayers tether to an apical actomyosin ring form the zonula adherens (ZA). We have previously shown that junction protein PLEKHA7 associates with and regulates function of core RNA interference (RNAi) component AGO2 specifically at ZA. However, mechanism mediating recruitment ZA remained unexplored. Here, we reveal this ZA-specific depends on both structural tensile...
ABSTRACT Adherens junctions are cadherin-based structures critical for cellular architecture. E-cadherin in mature epithelial cell monolayers tether to an apical actomyosin ring form the zonula adherens (ZA). We have previously shown that junction protein PLEKHA7 associates with and regulates function of core RNA interference (RNAi) component AGO2 specifically at ZA. However, mechanism mediating Ago2 recruitment ZA remained unexplored. Here, we reveal this ZA-specific depends on both...
Colon cancer is the third most common and second deadliest type of in United States. A typical characteristic colon tumors compromised integrity colonic epithelial monolayer. The Adherens Junction (AJ) a cell-cell adhesion complex essential for maintenance integrity, composed E-cadherin catenin family proteins. We have recently shown that E-cadherin-p120 partner PLEKHA7 critical monolayer integrity. also revealed recruits core components RNA interference (RNAi) machinery, such as Ago2,...
Abstract Colon cancer is the third most common and second deadliest type of in United States. A typical characteristic colon tumors compromised integrity colonic epithelial monolayer. The Adherens Junction (AJ) a cell-cell adhesion complex essential for maintenance integrity, composed E-cadherin catenin family proteins. We have recently shown that E-cadherin-p120 partner PLEKHA7 critical monolayer integrity. also revealed recruits core components RNA interference (RNAi) machinery, such as...