A5033231162- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- GDF15 and Related Biomarkers
- Nuclear Receptors and Signaling
- Peroxisome Proliferator-Activated Receptors
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Macrophage Migration Inhibitory Factor
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Peptidase Inhibition and Analysis
- Inflammatory mediators and NSAID effects
- Cancer, Lipids, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Tea Polyphenols and Effects
- Genetics, Aging, and Longevity in Model Organisms
- Tannin, Tannase and Anticancer Activities
- Enzyme Production and Characterization
- TGF-β signaling in diseases
- Morinda citrifolia extract uses
- ATP Synthase and ATPases Research
- Connective Tissue Growth Factor Research
Medical University of South Carolina
2017-2025
Gangneung–Wonju National University
2012-2025
University of Tennessee at Knoxville
2012-2016
University of Maryland, College Park
2013
Northwest A&F University
2013
Eulji University
2010
Summary Gene expression is dynamically regulated in a variety of mammalian physiologies. During aging, there are changes that occur protein highly controlled by the regulatory steps transcription, post‐transcription, and post‐translation. Although global profiles human transcripts during aging processes available, mechanism(s) which differentially expressed between young old cohorts remains unclear. Here, we report on N6‐methyladenosine (m6A) RNA modification peripheral blood mononuclear...
Long non-coding RNAs (lncRNAs) regulate vital biological processes, including cell proliferation, differentiation and development. A subclass of lncRNAs is synthesized from microRNA (miRNA) host genes (MIRHGs) due to pre-miRNA processing, are categorized as miRNA-host gene (lnc-miRHGs). Presently, the cellular function most lnc-miRHGs not well understood. We demonstrate a miRNA-independent role for nuclear-enriched lnc-miRHG in cycle progression. MIR100HG produces spliced stable that display...
Although Argonaute (AGO) proteins have been the focus of microRNA (miRNA) studies, we observed AGO-free mature miRNAs directly interacting with RNA-binding proteins, implying sophisticated nature fine-tuning gene regulation by miRNAs. To investigate microRNA-binding (miRBPs) globally, analyzed PAR-CLIP data sets to identify RBP quaking (QKI) as a novel miRBP for let-7b. Potential existence were further verified measuring miRNA levels in genetically engineered AGO-depleted human and mouse...
ABSTRACT The metazoan lifespan is determined in part by a complex signaling network that regulates energy metabolism and stress responses. Key hubs this include insulin/IGF‐1, AMPK, mTOR, sirtuins. Hippo/Mammalian Ste20‐like Kinase1 (MST1) pathway has been reported to maintain Caenorhabditis elegans , but its role not studied higher metazoans. In study, we report overexpression of Hpo, the MST1 homolog Drosophila melanogaster decreased with concomitant changes lipid aging‐associated gene...
Eukaryotic mRNA decay is tightly modulated by RNA-binding proteins (RBPs) and microRNAs (miRNAs). RBP AU-binding factor 1 (AUF1) has four isoforms resulting from alternative splicing critical for miRNA-mediated gene silencing with a distinct preference of target miRNAs. Previously, we have shown that AUF1 facilitates miRNA loading to Argonaute 2 (AGO2), the catalytic component RNA-induced complex. Here, further demonstrate depletion abolishes global interaction miRNAs AGO2. Single-molecule...
Chromolaena odorata (L.) King and Robinson (Siam weed) extract has been used to stop bleeding in wound healing many tropical countries. However, its detailed mechanisms have not elucidated. In this study, we examined the molecular by which Siam weed (SWE) affected hemostatic activities. SWE promoted Balb/c 3T3 fibroblast cell migration proliferation. Subsequently, found that heme oxygenase-1 (HO-1), accelerating enzyme, was increased at transcriptional translational levels treatments. The...
Abstract Inflammatory cytokines are key signaling molecules that can promote an immune response, thus their RNA turnover must be tightly controlled during infection. Most studies investigate the decay pathways in cytosol or nucleoplasm but never focused on nucleolus. Although this organelle has well-studied roles ribosome biogenesis and cellular stress sensing, mechanism of within nucleolus is not completely understood. Here, we report essential site inflammatory pre-mRNA instability...
Abstract Background The microRNAs (miRNAs) down‐regulated in aged mouse skeletal muscle were mainly clustered within the delta‐like homologue 1 and type III iodothyronine deiodinase ( Dlk1‐Dio3 ) genomic region. Although miRNAs are coexpressed regulate multiple targets a specific signalling pathway, function of cluster aging is largely unknown. We aimed to ascertain whether these play common role age‐related atrophy. Methods To examine anti‐atrophic effect miRNAs, we individually transfected...
Morinda citrifolia L. (Rubiaceae), commonly called noni, is a traditional folk medicinal plant with long history of use for several diseases. Its anti-inflammation activity has been proposed, but detailed knowledge this antiinflammation mechanism remains unclear. Here, we investigated the effects noni extract and its major bioactive component damnacanthal on in vivo as well vitro. Our data demonstrate that exhibit suppression inflammation evidenced by paw ear edema rats mice, down-regulation...
Abstract The nonsteroidal anti-inflammatory drug tolfenamic acid has been shown to suppress cancer cell growth and tumorigenesis in different models. However, the underlying mechanism by which exerts its antitumorigenic effect remains unclear. Previous data from our group others indicate that alters expression of apoptosis- cell-cycle arrest–related genes colorectal cells. Here, we show markedly reduced number polyps tumor load APCmin/+ mice, accompanied with cyclin D1 downregulation vitro...
Evaluating increasing circulating adiponectin levels is becoming an important strategy in the prevention of diabetes mellitus and cardiovascular events. This study was designed to investigate effect angiotensin II receptor blocker valsartan on blood insulin sensitivity patients with type 2 mild-to-moderate hypertension. A total 91 Korean were treated 80 mg/day for 4 weeks followed by 160 a further 8 weeks. Blood pressure, metabolic parameters measured before after treatment. The homeostasis...
Transforming growth factor-β (TGF-β) modulates diverse cell physiological processes and plays a complicated role in tumor development. It has been well established that TGF-β inhibits proliferation normal early stage carcinoma facilitates metastasis late-stage carcinoma. Therefore, blocking signaling advanced carcinogenesis provides potentially interesting chemotherapeutic strategy. We aimed to determine the effect of tolfenamic acid (TA) on TGF-β-induced protumorigenic activity. Here, we...
Post-transcriptional gene regulation is an important step in the of eukaryotic expression. Subcellular compartmentalization RNA species plays a crucial role control mRNA turnover, spatial restriction protein synthesis, and formation macromolecular complexes. Although long noncoding RNAs (lncRNAs) are one key regulators post-transcriptional expression, it not heavily studied whether localization lncRNAs subcellular organelles has functional consequences. Here, we report on mitochondrial whose...
Argonaute (AGO), a component of RNA-induced silencing complexes (RISCs), is representative RNA-binding protein (RBP) known to bind with mature microRNA (miRNA) and directly involved in post-transcriptional gene silencing. However, despite the biological significance miRNA, roles other micro proteins (miRBPs) remain unclear regulation miRNA loading, dissociation from RISC, extracellular release. In this study, we perform arrays profile miRBPs identify 118 binding miRNAs. Among those proteins,...
Fibrolamellar hepatocellular carcinoma (FLC) is a disease that occurs in children and young adults. The development of FLC associated with creation fusion oncoprotein DNAJB1-PKAc kinase, which activates multiple cancer-associated pathways. aim this study was to examine the role human genomic regions, called cancer-enhancing regions or aggressive liver cancer domains (CEGRs/ALCDs), FLC. Previous studies revealed CEGRs/ALCDs are located oncogenes genes, regularly silenced normal tissues. Using...