A5049347388- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant Molecular Biology Research
- Protist diversity and phylogeny
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Resistance
- Ion Channels and Receptors
- Plant Water Relations and Carbon Dynamics
- Plant-Microbe Interactions and Immunity
- Lipid metabolism and biosynthesis
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Mitochondrial Function and Pathology
- Emergency and Acute Care Studies
- Yeasts and Rust Fungi Studies
- Turfgrass Adaptation and Management
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
- Plant nutrient uptake and metabolism
- Plant Disease Management Techniques
- Leaf Properties and Growth Measurement
- Energy Efficiency and Management
- Pneumonia and Respiratory Infections
- Plant Gene Expression Analysis
- Chronic Obstructive Pulmonary Disease (COPD) Research
University of Cambridge
2015-2023
NIHR Surgical Reconstruction and Microbiology Research Centre
2021
Carnegie Institution for Science
2016-2019
Carnegie Department of Plant Biology
2016-2019
New Mexico State University
2013-2014
Doil Choi and colleagues report the genome sequence of hot pepper, Capsicum annuum, as well resequencing two cultivated peppers a wild species, chinense. Comparative genomic analysis across Solanaceae provides insights into expansion, pungency, ripening disease resistance in peppers. Hot pepper (Capsicum annuum), one oldest domesticated crops Americas, is most widely grown spice crop world. We whole-genome sequencing assembly (Mexican landrace annuum cv. CM334) at 186.6× coverage. also de...
Significance Eukaryotic algae, which play a fundamental role in global CO 2 fixation, enhance the performance of carbon-fixing enzyme Rubisco by placing it into an organelle called pyrenoid. Despite ubiquitous presence and biogeochemical importance this organelle, how assembles to form pyrenoid remains long-standing mystery. Our discovery abundant repeat protein that binds represents critical advance our understanding biogenesis. The sequence suggests elegant models explain structural...
A phase-separated, liquid-like organelle called the pyrenoid mediates CO
If the highly efficient C 4 photosynthesis pathway could be transferred to crops with 3 there yield gains of up 50%. It has been proposed that multiple metabolic and developmental modifications associated are underpinned by relatively few master regulators have allowed evolution more than 60 times in flowering plants. Here we identify a component one such regulator consists pair cis -elements located coding sequence genes preferentially expressed bundle sheath cells leaves. These motifs...
MicroRNAs (miRNAs) are a class of non-coding RNAs approximately 21 nt in length which play important roles regulating gene expression plants. Although many miRNA studies have focused on few model plants, miRNAs and their target genes remain largely unknown hot pepper (Capsicum annuum), one the most crops cultivated worldwide. Here, we employed high-throughput sequencing technology to identify extensively from 10 different libraries, including leaf, stem, root, flower, six developmental stage...
This study established the heat levels for several high-heat chile pepper ( Capsicum chinense ) varieties (Trinidad Moruga Scorpion, Bhut Jolokia, Trinidad Douglah Chocolate, and 7-pot Jonah) to determine which variety has highest average measured level relatedness of peppers using molecular analysis. In replicated trials with appropriate controls at Las Cruces, NM, results show two ‘Trinidad Scorpion’ plants reached more than million Scoville units (SHU). is first confirmation fruit...
Engineering C4 photosynthesis into C3 crops could substantially increase their yield by alleviating photorespiratory losses. This objective is challenging because the pathway involves complex modifications to biochemistry, cell biology, and anatomy of leaves. Forward genetics has provided limited insight mechanistic basis these properties, there have been no reports significant quantitative intraspecific variation attributes that would allow trait mapping. Here, we show accessions species...
C4 photosynthesis has evolved by repurposing enzymes found in C3 plants. Compared with the ancestral state, accumulation of cycle proteins is enhanced. We used de-etiolation C4Gynandropsis gynandra and C3Arabidopsis thaliana to understand this process. gene expression chloroplast biogenesis G. were tightly coordinated. Although genes showed similar induction patterns, gynandra, more strongly induced than orthologs from A. thaliana. In vivo binding TGA homeodomain as well light-responsive...
A novel disease resistance inhibitor gene (inhibitor of P. capsici [Ipcr]), found in the chile pepper (Capsicum annuum) variety ‘New Mexico Capsicum Accession 10399’ (NMCA10399), inhibits to Phytophthora but not other species Phytophthora. When a highly capsici-resistant was hybridized with NMCA10399, resultant F 1 populations, when screened, were completely susceptible for root rot and foliar blight syndromes, despite dominance inheritance pepper. The 2 population displayed 3:13...
Reducing photorespiration in C3 crops could significantly increase rates of photosynthesis and yield. One method to achieve this would be integrate C4 into species. This objective is challenging as it involves engineering incompletely understood traits leaves, including complex changes their biochemistry, cell biology, anatomy. Quantitative genetics selective breeding offer underexplored routes identify regulators these processes. We first review examples natural intraspecific variation well...
Abstract A sustainable food supply is vital for addressing the challenges of a growing global population and climate change. Gynandropsis gynandra , nutritious C 4 orphan crop native to Africa Asia, thrives in low-input agricultural systems, making it valuable candidate future security. This also serves as model studying photosynthesis. However, research on its genetic improvement limited. In this study, two mapping populations from diverse parental lines were used identify Quantitative...
Engineering C4 photosynthesis into C3 crops such as rice or wheat could substantially increase their yield by alleviating photorespiratory losses1,2. This objective is challenging because the pathway involves complex modifications to biochemistry, cell biology and anatomy of leaves3. Forward genetics has provided limited insight mechanistic basis these characteristics there have been no reports significant quantitative intra-specific variation attributes that would allow trait-mapping4,5....
ABSTRACT C 4 photosynthesis has evolved independently in over sixty lineages and so doing repurposed existing enzymes to drive a carbon pump that limits the RuBisCO oxygenation reaction. In all cases, gene expression is modified such proteins accumulate levels matching those of photosynthetic apparatus. To better understand this rewiring we undertook RNA- DNaseI-SEQ on de-etiolating seedlings Gynandropsis gynandra , which sister 3 Arabidopsis. Changes chloroplast ultrastructure were...
Engineering C 4 photosynthesis into 3 crops such as rice or wheat could substantially increase their yield by alleviating photorespiratory losses 1,2 . This objective is challenging because the pathway involves complex modifications to biochemistry, cell biology and anatomy of leaves Forward genetics has provided limited insight mechanistic basis these characteristics there have been no reports significant quantitative intra-specific variation attributes that would allow trait-mapping 4,5...
This article comments on: van Rooijen R, Schulze S, Petzsch P, Westhoff P. 2020. Targeted misexpression of NAC052, acting in H3K4 demethylation, alters leaf morphological and anatomical traits Arabidopsis thaliana. Journal Experimental Botany 71, 1434–1448.
<h3>Introduction and Objectives</h3> Digital technology with capability for virtual ward consultant clinical supervision, DECAF scoring a trusted assessor model was used to select patients early supported discharge (ESD). We present here data on the initial three months of pilot ESD service. <h3>Methods</h3> joint venture combined workforce established hospital respiratory specialists (Respiratory Support Team; RST) an expanded community team (CRT). A robust alert system linked our...