A5067138195- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Light effects on plants
- Plant Parasitism and Resistance
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Neurobiology and Insect Physiology Research
- Biochemical and biochemical processes
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Plant responses to elevated CO2
- Microbial Community Ecology and Physiology
- Heat shock proteins research
- Soil Carbon and Nitrogen Dynamics
- Plant Reproductive Biology
- Plant Diversity and Evolution
- Insect and Arachnid Ecology and Behavior
- Insect Resistance and Genetics
University of Cambridge
2017-2024
Phillips Exeter Academy
2016-2019
Sainsbury Laboratory
2017-2018
Chloroplast biogenesis is dependent on master regulators from the GOLDEN2-LIKE (GLK) family of transcription factors. However, glk mutants contain residual chlorophyll, indicating that other proteins must be involved. Here, we identify MYB-related factors as chloroplast in liverwort Marchantia polymorpha and angiosperm Arabidopsis thaliana. In both species, double-mutant alleles genes show very limited development, photosynthesis gene expression perturbed to a greater extent than GLK...
Plants have significantly more transcription factor (TF) families than animals and fungi, plant TF tend to contain genes; these expansions are linked adaptation environmental stressors. Many family members bind similar or identical sequence motifs, such as G-boxes (CACGTG), so it is difficult predict regulatory relationships. We determined that the flanking sequences near help determine in vitro specificity but this insufficient pattern of genes G-boxes. Therefore, we constructed a gene...
Temperature is a key environmental variable influencing plant growth and survival. Protection against high temperature stress in eukaryotes coordinated by heat shock factors (HSFs), transcription that activate the expression of protective chaperones such as HEAT SHOCK PROTEIN 70 (HSP70); however, pathway which sensed integrated with other signals into adaptive responses not well understood. Plants are exposed to considerable diurnal variation temperature, we have found there thermotolerance...
Abstract Leaves of shade-avoiding plants such as Arabidopsis (Arabidopsis thaliana) change their growth pattern and position in response to low red far-red ratios (LRFRs) encountered dense plant communities. Under LRFR, transcription factors the phytochrome-interacting factor (PIF) family are derepressed. PIFs induce auxin production, which is required for promoting leaf hyponasty, thereby favoring access unfiltered sunlight. Abscisic acid (ABA) has also been implicated control with gene...
C4 photosynthesis has evolved by repurposing enzymes found in C3 plants. Compared with the ancestral state, accumulation of cycle proteins is enhanced. We used de-etiolation C4Gynandropsis gynandra and C3Arabidopsis thaliana to understand this process. gene expression chloroplast biogenesis G. were tightly coordinated. Although genes showed similar induction patterns, gynandra, more strongly induced than orthologs from A. thaliana. In vivo binding TGA homeodomain as well light-responsive...
Compared with the ancestral C3 state, C4 photosynthesis occurs at higher rates improved water and nitrogen use efficiencies. In both plants, of increase light intensity are maximal around midday. We determined that in absence or temperature fluctuations, maize (Zea mays) peaks middle subjective photoperiod. To investigate molecular processes associated these temporal changes, we performed RNA sequencing mesophyll bundle sheath strands over a 24-h time course. Preferential expression cycle...
Binary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology, physiology. However, number of well-defined LexA enhancer trap insertions remains limited. In this study, we present molecular characterization initial analysis nearly 100 novel StanEx traps, derived from
Abstract Chloroplast biogenesis is dependent on master regulators from the GOLDEN2-LIKE (GLK) family of transcription factors, but glk mutants contain residual chlorophyll and therefore other proteins must also be involved. Here we identify MYB-related factors as chloroplast in liverwort Marchantia polymorpha angiosperm Arabidopsis thaliana . In both species, double mutant alleles MYB-Related genes show very limited development, photosynthesis gene expression perturbed to a greater extent...
Abstract In C 4 plants, the enzymatic machinery underpinning photosynthesis can vary, with, for example, three distinct acid decarboxylases being used to release CO 2 in vicinity of RuBisCO. For decades, these have been classify species into biochemical sub‐types. However, more recently, notion that mix and match has increased popularity, as a consequence, validity specific sub‐types questioned. Using five from grass tribe Paniceae, we show that, although some transcripts enzymes involved...
Abstract In C 4 plants, the enzymatic machinery underpinning photosynthesis can vary, with, for example, three distinct acid decarboxylases being used to release CO 2 in vicinity of RuBisCO. For decades, these have been classify species into biochemical sub-types. However, more recently notion that mix and match has increased popularity and, as a consequence, validity specific sub-types questioned. Using five from grass tribe Paniceae, we show that, while some transcripts encoding multiple...
ABSTRACT C 4 photosynthesis has arisen from the ancestral 3 state in over sixty lineages of angio-sperms. It is widely accepted that an early step evolution restriction glycine decarboxylase activity to bundle sheath cells generate so-called 2 pathway. In Moricandia species, changes cis -regulatory region controlling expression P-subunit GLYCINE DECARBOXYLASE ( GLDP ) mesophyll enables this trait, but mechanism underpinning not known. We identify a MYC-MYB transcription factor module...
Abstract Leaves of shade avoiding plants like Arabidopsis thaliana change their growth pattern and position in response to a low red far-red ratio (LRFR) that is encountered dense plant communities. In LRFR, transcription factors the phytochrome interacting family (PIFs) are de-repressed. PIFs induce auxin production, which required promote leaf hyponasty thereby favoring access unfiltered sunlight. Abscisic acid (ABA) has also been implicated control hyponasty. addition, gene expression...
ABSTRACT Plants have significantly more transcription factor (TF) families than animals and fungi, plant TF tend to contain genes—these expansions are linked adaptation environmental stressors (1, 2). Many family members bind similar or identical sequence motifs, such as G-boxes (CACGTG), so it is difficult predict regulatory relationships. We determine that the flanking sequences near help in vitro specificity, but this insufficient pattern of genes G-boxes. Therefore, we construct a gene...
As DNA sequencing has become more popular, knowledge about the soil microbiome expanded considerably. This technological advancement led to a greatly increased understanding role of bacteria in soil. Previous studies have shown that, while geographic proximity plays minor determining species richness (1), there exists direct correlation between microbial diversity and both pH (1) plant variety (2). In this paper, we examine content samples taken from three locations southern New Hampshire...
Abstract Binary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology physiology. However, number of well-defined LexA enhancer trap insertions remains limited. In this study, we present molecular characterization initial analysis nearly 100 novel StanEx traps, derived from StonEx 1 index line. This includes 76 into novel, distinct loci not previously associated with traps or targeted...