A5042839186- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Bryophyte Studies and Records
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- RNA Interference and Gene Delivery
- Mycorrhizal Fungi and Plant Interactions
- Light effects on plants
- Advanced biosensing and bioanalysis techniques
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Advancements in Photolithography Techniques
- Osteomyelitis and Bone Disorders Research
- RNA modifications and cancer
- Botany and Plant Ecology Studies
- Plant nutrient uptake and metabolism
- Pregnancy and preeclampsia studies
- Enzyme Production and Characterization
- VLSI and Analog Circuit Testing
- Forest, Soil, and Plant Ecology in China
University of Cambridge
2017-2024
University of Florence
2023
Meyer Children's Hospital
2023
Università Cattolica del Sacro Cuore
2020
Istituti di Ricovero e Cura a Carattere Scientifico
2020
Earlham Institute
2019
Plant (United States)
2019
Sainsbury Laboratory
2017-2018
Chloroplast biogenesis is dependent on master regulators from the GOLDEN2-LIKE (GLK) family of transcription factors. However, glk mutants contain residual chlorophyll, indicating that other proteins must be involved. Here, we identify MYB-related factors as chloroplast in liverwort Marchantia polymorpha and angiosperm Arabidopsis thaliana. In both species, double-mutant alleles genes show very limited development, photosynthesis gene expression perturbed to a greater extent than GLK...
We present the OpenPlant toolkit, a set of interlinked resources and techniques to develop Marchantia as testbed for bioengineering in plants. is liverwort, simple plant with an open form development that allows direct visualization gene expression dynamics cellular growth living tissues. describe new efficient axenic propagation maintenance lines no requirement glasshouse facilities. plants spontaneously produce clonal propagules within few weeks regeneration, can be amplified million-fold...
Abstract Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterizing transcriptional activity TF genes in space time is a critical step toward understanding complex biological systems. The vegetative gametophyte meristems bryophytes share some characteristics with shoot apical flowering plants. However, identity profiles TFs associated organization largely unknown. With only ∼450 putative genes, Marchantia (Marchantia...
Significance Despite the importance of liverworts as earliest diverging land plant lineage to support fungal symbiosis, it is unknown whether filamentous pathogens can establish intracellular interactions within living cells these nonvascular plants. Here, we demonstrate that an oomycete pathogen invades Marchantia polymorpha and related form infection structures inside photosynthetic layer. Plants lacking this tissue layer display enhanced resistance infection, revealing architectural...
Photosynthesis in eukaryotic cells takes place chloroplasts that develop from undifferentiated plastids response to light. Two families of transcription factors known as Golden2-like (GLK) and GATA regulate plant chloroplast development, the MIR171-targeted SCARECROW-LIKE (SCL) GRAS chlorophyll biosynthesis. Additionally, Elongated Hypocotyl 5 (HY5) factor plays a critical role photomorphogenesis. The extent which these proteins carry out conserved roles non-seed plants such liverworts is...
Abstract Chloroplast biogenesis is dependent on master regulators from the GOLDEN2-LIKE (GLK) family of transcription factors, but glk mutants contain residual chlorophyll and therefore other proteins must also be involved. Here we identify MYB-related factors as chloroplast in liverwort Marchantia polymorpha angiosperm Arabidopsis thaliana . In both species, double mutant alleles MYB-Related genes show very limited development, photosynthesis gene expression perturbed to a greater extent...
ABSTRACT The expansion of plants onto land was a formative event that brought forth profound changes to the Earth’s geochemistry and biota. Filamentous eukaryotic microbes developed ability colonize plant tissues early during evolution plants, as demonstrated by intimate symbiosis-like associations in >400 million-year-old fossils. However, degree which filamentous establish pathogenic interactions with divergent is unclear. Here, we demonstrate broad host-range oomycete pathogen...
Objective To ascertain the most effective approach in pregnancies complicated by mild intrahepatic cholestasis of pregnancy (mICP) evaluating rates adverse perinatal outcomes (APOs) and pathological placental findings.Methods A total 89 mICP (defined as serum bile acids (TSBAs) levels <40 µmol/L) were included. One-drug (ursodeoxycholic acid [UDCA]) (n = 49, 55.1%) combined (UDCA plus S-adenosyl methionine (SAMe)) 40, 44.9%) therapies compared.Results No differences found demographic,...
ABSTRACT Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterising transcriptional activity TF genes in space time is a critical step towards understanding complex biological systems. The vegetative gametophyte meristems bryophytes share some characteristics with shoot-apical flowering plants. However, identity profiles TFs associated organization largely unknown. With only ∼450 genes, Marchantia polymorpha an outstanding...
SUMMARY A combination of streamlined genetics, experimental tractability and relative morphological simplicity compared to vascular plants makes the liverwort Marchantia polymorpha an ideal model system for studying many aspects plant biology. Here we describe a transformation vector combining constitutive fluorescent membrane marker with nuclear that is regulated by nearby enhancer elements use this produce library trap lines . Screening gemmae from these allowed identification...
Protocol based on Pollak B, Cerda A, Delmans M, et al (2019) Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol 222:628–640 https://doi.org/10.1111/nph.15625
A modification of the Ishizaki et al 2008 Agrobacterium mediated Marchantia sporeling transformation protocol is used. Sterilised spores are grown for 5 days in 0.5x strength Gamborg plates prior to co-cultivation 2 with agrobacterium liquid media multiwell plates. Sporelings then spread on appropriate selective antibiotic. In about 7 days, positive transformants start emerge.
Summary A combination of streamlined genetics, experimental tractability and relative morphological simplicity compared to vascular plants makes the liverwort Marchantia polymorpha an ideal model system for studying many aspects plant biology. Here we describe a transformation vector combining constitutive fluorescent membrane marker with nuclear that is regulated by nearby enhancer elements, use this produce library trap lines Marchantia. Screening gemmae from these allowed identification...
Simplified cryopreservation protocol for Marchantia polymorpha gemmae, based on (Tanaka et al. 2016, Plant and Cell Physiology). Enables long term storage of gemmae at -80°C.
Protocol based on Pollak B, Cerda A, Delmans M, et al (2019) Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol 222:628–640 https://doi.org/10.1111/nph.15625
sites is necessary when a DNA sequence of interest contains an internal BsaI or SapI recognition site.For domestication, overlapping PCR can be protocols.
This protocol explains how to design and clone the guide RNA target sequence into a L2 plasmid ready accept gRNA by cloning with SapI (L2 also contains cassette express Cas9)
Protocol based on: Pollak B, Cerda A, Delmans M, et al (2019) Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol 222:628–640 https://doi.org/10.1111/nph.15625
Protocol based on: Pollak B, Cerda A, Delmans M, et al (2019) Loop assembly: a simple and open system for recursive fabrication of DNA circuits. New Phytol 222:628–640 https://doi.org/10.1111/nph.15625
This protocol explains how to design clone the guide RNA (gRNA) sequence into L2 plasmids ready accept gRNA by cloning with SapI. We have two versions of plasmid, or without Cas9: L2_gRNA-Cas9-CsA and L2_gRNA-CsA plasmids. With you transform wild-type sporelings. sporelings a line expressing Cas9.
This protocol explains how to design and clone the guide RNA target sequence into a L1 plasmid ready accept gRNA by cloning with BbsI. plasmids are L1_lacZgRNA-Ck2 L1_lacZgRNA-Ck3. If one is cloned Ck2 another Ck3 one, two L1_gRNA transcription units can be combined an antibiotic resistance unit MpEF1α:Cas9 via L2 SapI Loop assembly. allows for dual editing.