A5092212137- Plant tissue culture and regeneration
- Bryophyte Studies and Records
- Plant Molecular Biology Research
- Biocrusts and Microbial Ecology
- Botanical Research and Applications
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
University of Cambridge
2023-2025
Abstract Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterizing transcriptional activity TF genes in space time is a critical step toward understanding complex biological systems. The vegetative gametophyte meristems bryophytes share some characteristics with shoot apical flowering plants. However, identity profiles TFs associated organization largely unknown. With only ∼450 putative genes, Marchantia (Marchantia...
High-throughput experiments in plants are hindered by long generation times and high costs. To address these challenges, we present an optimized pipeline for Agrobacterium tumefaciens transformation a simplified protocol to obtain stable transgenic lines of the model liverwort Marchantia polymorpha, paving way efficient high-throughput plant synthetic biology other applications. Our involves freeze-thaw method six-well plates that can be adapted robotic automation. Using Opentrons...
Abstract Marchantia polymorpha has become an important model system for comparative studies and synthetic biology. The systematic characterization of genetic elements would make heterologous gene expression more predictable in this test bed circuit assembly bioproduction. Yet, the toolbox parts includes only a few constitutive promoters that need benchmarking to assess their utility. We compared patterns previously characterized new promoters. found driving with double enhancer version...
ABSTRACT Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterising transcriptional activity TF genes in space time is a critical step towards understanding complex biological systems. The vegetative gametophyte meristems bryophytes share some characteristics with shoot-apical flowering plants. However, identity profiles TFs associated organization largely unknown. With only ∼450 genes, Marchantia polymorpha an outstanding...
ABSTRACT High-throughput experiments in plants are hindered by long generation times and high costs. To address these challenges, we present an optimized pipeline for Agrobacterium tumefaciens transformation simplified a protocol to obtain stable transgenic lines of the model liverwort Marchantia polymorpha , paving way efficient high-throughput plant synthetic biology other applications. Our involves freeze-thaw method 6-well plates that can be adapted robotic automation. Using Opentrons...