A5038973927- Plant Molecular Biology Research
- Bryophyte Studies and Records
- Photosynthetic Processes and Mechanisms
- Acute Lymphoblastic Leukemia research
- Plant Reproductive Biology
- Plant Taxonomy and Phylogenetics
- Lung Cancer Research Studies
- PARP inhibition in cancer therapy
- Plant nutrient uptake and metabolism
- DNA Repair Mechanisms
- Chronic Myeloid Leukemia Treatments
- Light effects on plants
- Peatlands and Wetlands Ecology
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
British Columbia Children's Hospital
2023-2024
University of Cambridge
2020-2024
University of British Columbia
2022-2023
Abstract Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterizing transcriptional activity TF genes in space time is a critical step toward understanding complex biological systems. The vegetative gametophyte meristems bryophytes share some characteristics with shoot apical flowering plants. However, identity profiles TFs associated organization largely unknown. With only ∼450 putative genes, Marchantia (Marchantia...
SUMMARY Land plants comprise two large monophyletic lineages, the vascular and bryophytes, which diverged from their most recent common ancestor approximately 480 million years ago. Of three lineages of only mosses liverworts are systematically investigated, while hornworts understudied. Despite importance for understanding fundamental questions land plant evolution, they recently became amenable to experimental investigation, with Anthoceros agrestis being developed as a hornwort model...
Chloroplasts are attractive platforms for synthetic biology applications since they capable of driving very high levels transgene expression, if mRNA production and stability properly regulated. However, plastid transformation is a slow process currently limited to few plant species. The liverwort Marchantia polymorpha simple model that allows rapid studies; however, its potential protein hyperexpression has not been fully exploited. This partially due the fact chloroplast...
Photosynthesis in eukaryotic cells takes place chloroplasts that develop from undifferentiated plastids response to light. Two families of transcription factors known as Golden2-like (GLK) and GATA regulate plant chloroplast development, the MIR171-targeted SCARECROW-LIKE (SCL) GRAS chlorophyll biosynthesis. Additionally, Elongated Hypocotyl 5 (HY5) factor plays a critical role photomorphogenesis. The extent which these proteins carry out conserved roles non-seed plants such liverworts is...
Childhood acute lymphoblastic leukemia (ALL) genomes show that relapses often arise from subclonal outgrowths. However, the impact of clonal evolution on actionable proteome and response to targeted therapy is not known. Here, we present a comprehensive retrospective analysis paired ALL diagnosis relapsed specimen. Targeted next generation sequencing indicate persistence genome variants stable proteomes through disease progression. Paired viably-frozen biopsies high correlation drug...
Abstract The presence of supernumerary chromosomes is the only abnormality shared by all patients diagnosed with high-hyperdiploid B cell acute lymphoblastic leukemia (HD-ALL). Despite being most frequently pediatric leukemia, lack clonal molecular lesions and complete absence appropriate experimental models have impeded elucidation HD-ALL leukemogenesis. Here, we report that for 23 samples isolated from moribund Eμ-Ret mice, were characterized non-random chromosomal gains, involving...
Acute lymphoblastic leukemia (ALL) is the most common form of cancer in children, with cases arising from fetal B cell precursor, termed B-ALL. Here, we use immunofluorescence analysis B-ALL cells to identify centrosome amplification events that require clustering pathway successfully complete mitosis. Our data reveals primary human and immortal lines both mouse sources show defective bipolar spindle formation, abnormal mitotic progression, death following treatment inhibitors (CCI). We...
ABSTRACT Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterising transcriptional activity TF genes in space time is a critical step towards understanding complex biological systems. The vegetative gametophyte meristems bryophytes share some characteristics with shoot-apical flowering plants. However, identity profiles TFs associated organization largely unknown. With only ∼450 genes, Marchantia polymorpha an outstanding...
Abstract Land plants comprise two large monophyletic lineages, the vascular and bryophytes, which diverged from their most recent common ancestor approximately 480 million years ago. Of three lineages of only mosses liverworts are systematically investigated, while hornworts understudied. Despite importance for understanding fundamental questions land plant evolution, they recently became amenable to experimental investigation, with Anthoceros agrestis being developed as a hornwort model...
ABSTRACT Childhood acute lymphoblastic leukemia (ALL) genomes show that relapses often arise from subclonal outgrowths. However, the impact of clonal evolution on actionable proteome and response to targeted therapy is not known. Here, we present a comprehensive retrospective analysis paired ALL diagnosis relapsed specimen. Targeted next generation sequencing indicated persistence genome variants stable proteomes through disease progression. Paired viably-frozen biopsies showed high...
ABSTRACT Chloroplast genes are present at high ploidy in plants, and capable of driving very levels gene expression if mRNA production stability properly regulated. Marchantia polymorpha is a simple model plant that allows rapid transformation studies, however post-transcriptional regulation plastids poorly characterized this liverwort. We have mapped patterns transcription chloroplasts. Furthermore, we obtained compared sequences from 51 early-divergent species, identified putative sites...