A5102706753- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Plant nutrient uptake and metabolism
- Plant Taxonomy and Phylogenetics
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant Parasitism and Resistance
- Advanced Biosensing Techniques and Applications
- Single-cell and spatial transcriptomics
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- Advanced SAR Imaging Techniques
- Plant Gene Expression Analysis
- Advanced Graph Neural Networks
Jilin University
2019-2025
State Key Laboratory of Supramolecular Structure and Materials
2019-2025
First Hospital of Jilin University
2025
Qingdao Agricultural University
2017-2024
State Forestry and Grassland Administration
2024
Jilin Medical University
2024
Affiliated Zhongshan Hospital of Dalian University
2018-2023
Genomics (United Kingdom)
2023
Berry Oncology (China)
2023
Chinese Academy of Agricultural Sciences
2015-2020
A microdroplet-based surface-enhanced Raman spectroscopy (microdroplet SERS) platform was constructed to envelop individual cells in microdroplets, followed by the SERS detection of their extracellular vesicle-proteins (EV-proteins) via in-drop immunoassays use immunomagnetic beads (iMBs) and immuno-SERS tags (iSERS tags). unique phenomenon is found that iMBs can start a spontaneous reorientation on probed cell surface based electrostatic force-driven interfacial aggregation effect, which...
Alfalfa (Medicago sativa L.) is a perennial flowering plant in the legume family that widely cultivated as forage crop for its high yield, quality and related agricultural economic benefits. photoperiod sensitive long-day (LD) can accomplish vegetative reproductive phases short period of time. However, rapid compromise biomass yield quality. Here, we attempted to delay alfalfa using multiplex CRISPR/Cas9-mediated mutagenesis FLOWERING LOCUS Ta1 (MsFTa1), key floral integrator activator gene....
Abstract Main conclusion An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-driven Cas9 exhibits consistently high mutation efficiency in and M. truncatula. is a powerful genome editing technology that has been applied several crop species for trait improvement due to its simplicity, versatility, specificity. However, of truncatula (Mt) still challenging inconsistent. To analyze functionality two model dicot species, four different systems target phytoene desaturase ( PDS )...
Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system has been successfully used for genome editing many plant species. However, use of CRISPR/Cas9 gene knockout alfalfa still very challenging. Our initial single gRNA-CRISPR/Cas9 had low mutagenesis efficiency with no mutant phenotype. In order to develop optimized alfalfa, we...
Salt stress is an important abiotic that causes decreased crop yields. Root growth and plant activities are affected by salt through the actions of specific genes help roots adapt to adverse environmental conditions. For a more comprehensive understanding proteins salinity, we used two-dimensional gel electrophoresis mass spectrometry characterize proteome-level changes associated with response in Medicago sativa cv. Zhongmu-1 truncatula Jemalong A17 roots. Our physiological phenotypic...
Alfalfa ( Medicago sativa L.) forage quality is adversely affected by lignin deposition in cell walls at advanced maturity stages. Reducing content through RNA interference or antisense approaches has been shown to improve alfalfa and digestibility. We employed a multiplex CRISPR/Cas9-mediated gene-editing system reduce alter composition targeting the COUMARATE 3-HYDROXYLASE (MsC3H) gene, which encodes key enzyme biosynthesis. Four guide RNAs (gRNAs) first exon of MsC3H were designed...
Alkaline phosphatase (ALP) is a biomarker for many diseases, and monitoring its activity level important disease diagnosis treatment. In this study, we used the microdroplet technology combined with an in situ laser-induced polymerization method to prepare Ag nanoparticle (AgNP) doped hydrogel microbeads (HMBs) adjustable pore sizes that allow small molecules enter while blocking large molecules. The AgNPs embedded microspheres can provide SERS activity, improving signal of diffuse AgNPs. A...
Alfalfa (Medicago sativa L.) is a perennial leguminous forage cultivated globally. Fusarium spp.-induced root rot chronic and devastating disease affecting alfalfa that occurs in most production fields. Studying the resistance regulatory network investigating key genes involved plant–pathogen can provide vital information for breeding are resistant to spp. In this study, susceptible clonal line of was inoculated with proliferatum L1 sampled at 24 h, 48 72 7 d post-inoculation RNA-seq...
Despite recent advances in single-cell analysis techniques, the ability of platforms to track specific cells that secreted cytokines remains limited. Here, we report a microfluidic droplet-based fluorescence imaging platform can analyze single cell-secreted vascular endothelial growth factor (VEGF), an important regulator physiological and pathological angiogenesis, explore cellular clues at level. Two kinds silica nanoparticle (NP)-based immunoprobes were developed, they bioconjugated...
The long-term survival of alfalfa (Medicago sativa L.) is limited by several factors, including Fusarium root rot (FRR) caused species. Resistance to FRR in usually evaluated under field conditions or a soil culture test conducted greenhouse artificially inoculated soil, both which involve the assessment disease severity index. A new hydroponics screening (HS) method was developed more efficiently screen large population germplasm for resistance FRR. Spearman correlation analysis revealed...
Phosphite, a reduced form of orthophosphate, is characterized by high solubility, and transportation efficiency can be used as potential phosphorus fertilizer, plant biostimulant supplemental fertilizer in agriculture. However, the effects phosphite on soil properties microorganisms are poorly understood. This study evaluated phosphate fertilizers different forms phosphorus, alkaline phosphatase (ALP) activity, phoD -harboring bacterial community alfalfa ( Medicago sativa ) field. The four...
Alfalfa sprouts are among the most nutritionally rich foods, and light exposure is a critical factor in determining their biomass quality. However, detailed metabolic molecular differences between yellow green alfalfa remain unclear. In this study, comprehensive metabolomic transcriptomic analyses were integrated to evaluate nutrient composition of during germination with or without exposure. Differentially expressed genes differentially accumulated metabolites significantly enriched...
In plants, the leaf is an essential photosynthetic organ, and primary harvest in forage crops such as alfalfa (
Abstract As the "powerhouse" of cells, mitochondrion is main site cellular aerobic respiration and plays a vital role in performing many important functions. The consequence mitochondrial dysfunction could lead to wide range disorders diseases. Traditional mitochondria‐related cytobiology methods always require isolation organelles for biological components, followed by structural analysis quantitative analysis. In this study, we tried assess feasibility reliability isolation‐based method...
This case–control study was aimed to evaluate the influence of cytochrome P450 family 17 subfamily A member 1 (CYP17A1) gene rs743572 polymorphism for susceptibility endometriosis. Polymerase chain reaction-restriction fragment length (PCR-RFLP) used genotype in 143 endometriosis patients and 148 healthy controls. Hardy–Weinberg equilibrium (HWE) test utilized detect representativeness subjects. Association strength presented by odds ratios (ORs) with corresponding 95% confidence intervals...
Abstract The genetic diversity of the Vicia ( L.) genus was analyzed using simple sequence repeat (SSR) markers. A total 201 sampled individuals from 12 were assayed to study and polymorphisms markers; 115 alleles identified. average observed heterozygosity (Ho) expected (He) 0.4283 0.6941, respectively. polymorphic information content (PIC) varied 0.8739 (V81) 0.9579 (V97), with a mean 0.9033, indicating that markers highly informative. Moreover, cluster principal coordinate analysis (PCoA)...
Leaf size significantly impacts photosynthetic capacity and forage yield in alfalfa, a major legume crop. Therefore, elucidating the genetic factors governing leaf development is critical for breeding improved alfalfa varieties. In this study, genome-wide association analysis (GWAS) was performed to dissect architecture of length (LL) width (LW) using 220 accessions phenotyped over three years. Substantial variation both traits observed across environments, with coefficients ranging from...
Paspalum notatum 'Flugge' is a diploid with 20 chromosomes (2n = 20) multi-purpose subtropical herb native to South America and has high ecological significance. It currently widely planted in tropical regions. Despite the gene pool of P. being unearthed large extent past decade, no details about genomic information relevant species have been reported. In this study, complete genome was established annotated through sequencing de novo assembly its genome.The latest PacBio third-generation...
Alfalfa (Medicago sativa L.) is an important forage crop and also a target of many fungal diseases including Fusarium spp. As today, very little information available about molecular mechanisms that contribute to pathogenesis defense responses in alfalfa against specifically proliferatum, the causal agent root rot. In this study, we used proteomic approach identify inducible proteins during compatible interaction with F. proliferatum strain YQC-L1. Samples for two-dimensional gel...
Abstract The legume plant alfalfa ( Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability diverse agro-ecological zones. Alfalfa self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore, maintaining excellent traits through seed reproduction prime challenge in alfalfa. However, the cutting propagation technology could enable consistent multiplication of quality plants that are...