A5037783008- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Probiotics and Fermented Foods
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Microbial Metabolites in Food Biotechnology
- CRISPR and Genetic Engineering
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolism and Applications
- Advanced Cellulose Research Studies
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Fungal Biology and Applications
- Enzyme Structure and Function
- Food composition and properties
- Microbial Fuel Cells and Bioremediation
- Polymer Nanocomposite Synthesis and Irradiation
- Microbial infections and disease research
- Mycorrhizal Fungi and Plant Interactions
- Arsenic contamination and mitigation
- Biochemical and biochemical processes
University of Naples Federico II
2014-2025
National Research Council
2024
Institute of Polymers, Composites and Biomaterials
2024
Lawrence Berkeley National Laboratory
2020-2023
Joint BioEnergy Institute
2020-2022
Chalmers University of Technology
2019
Abstract Agri-food residues offer significant potential as a raw material for the production of L -lactic acid through microbial fermentation. Weizmannia coagulans , previously known Bacillus is spore-forming, lactic acid-producing, gram-positive, with probiotic and prebiotic properties. This study aimed to evaluate feasibility utilizing untreated citrus waste sustainable feedstock in one-step process, by using strain W. MA-13. By employing thermophilic enzymatic cocktail (Cellic CTec2)...
The transition from a petroleum-based economy towards more sustainable bioprocesses for the production of fuels and chemicals (circular economy) is necessary to alleviate impact anthropic activities on global ecosystem. Lignocellulosic biomass-derived sugars are suitable alternative feedstocks that can be fermented or biochemically converted value-added products. An example lactic acid, which an essential chemical polylactic biodegradable bioplastic. However, acid still mainly produced by...
A novel endo-1,4-β-xylanase-encoding gene was identified in Alicyclobacillus mali FL18 and the recombinant protein, named AmXyn, purified biochemically characterized. The monomeric enzyme worked optimally at pH 6.6 80 °C on beechwood xylan with a specific activity of 440.00 ± 0.02 U/mg good catalytic efficiency (kcat/KM = 91.89 s−1mLmg−1). In addition, did not display any cellulose, suggesting possible application paper biobleaching processes. To develop an enzymatic mixture for degradation,...
Lignocellulose biomasses (LCB), including spent mushroom substrate (SMS), pose environmental challenges if not properly managed. At the same time, these renewable resources hold immense potential for biofuel and chemicals production. With market growth expected to amplify SMS quantities, repurposing or disposal strategies are critical. This study explores use of cultivating microbial communities produce carbohydrate-active enzymes (CAZymes). Addressing a research gap in using anaerobic...
The genus Thermus, which has been considered for a long time as fruitful source of biotechnological relevant enzymes, emerged more recently suitable host to overproduce thermozymes. Among these, α-galactosidases are widely used in several industrial bioprocesses that require high working temperatures and thermostable variants offer considerable advantages over their thermolabile counterparts. Thermus thermophilus HB27 strain was the homologous expression TTP0072 gene encoding an...
Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect growth metabolism are released from upon thermochemical pre-treatment. So far, susceptibility bacterial strains to biomass-derived inhibitors still major barrier lignocellulose. Detoxification pre-treated lignocellulosic by water washing commonly performed alleviate inhibition...
The production of biochemicals requires the use microbial strains with efficient substrate conversion and excellent environmental robustness, such as Weizmannia coagulans species. So far, genomes 47 have been sequenced. Herein, we report a comparative genomic analysis nine on full repertoire Carbohydrate-Active enZymes (CAZymes), secretion systems, resistance mechanisms to challenges. Moreover, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) immune system along...
Abstract Background Plant cell walls are interwoven structures recalcitrant to degradation. Native and adapted microbiomes can be particularly effective at plant wall deconstruction. Although most understanding of biological deconstruction has been obtained from isolates, cultivated that break down have emerged as new sources for biotechnologically relevant microbes enzymes. These provide a unique resource identify key interacting functional microbial groups guide the design specialized...
Abstract Background The spore-forming lactic acid bacterium Bacillus coagulans MA-13 has been isolated from canned beans manufacturing and successfully employed for the sustainable production of lignocellulosic biomass. Among bacteria, B. strains are generally recognized as safe (GRAS) human consumption. Low-cost microbial industrially valuable products such various enzymes devoted to hydrolysis oligosaccharides lactose, is great importance food industry. Specifically, α- β-galactosidases...
Extreme environments are excellent places to find microorganisms capable of tolerating extreme temperature, pH, salinity pressure, and elevated concentration heavy metals other toxic compounds. In the last decades, extremophilic have been extensively studied since they can be applied in several fields biotechnology along with their enzymes. this context, characterization metal resistance determinants thermophilic is starting point for development new biosystems bioprocesses environmental...
Nanostructured cellulose (NC) represents an emerging sustainable biomaterial for diverse biotechnological applications; however, its production requires hazardous chemicals that render the process ecologically unfriendly. Using commercial plant-derived cellulose, innovative strategy NC based on combination of mechanical and enzymatic approaches was proposed as a alternative to conventional chemical procedures. After ball milling, average length fibers reduced by one order magnitude (down...
The isolation of novel microbes from environmental samples continues to be a key strategy for the discovery new metabolic capacities degradation and transformation lignocellulose. We report draft genome sequence strain Brevibacillus borstelensis isolated sorghum-adapted microbial community derived compost sample.
Bacillus coagulans MA-13 is an efficient lactic acid producer which withstands high concentrations of the growth inhibitors formed during pretreatment lignocellulosic feedstock. This draft genome sequence expected to pave way toward understanding mechanisms responsible for robustness simultaneous saccharification and fermentation.
Abstract Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin. Analytical tools capable quickly detecting both glycan lignin deconstruction are needed to support the development characterization efficient enzymes/enzyme cocktails. Previously we have described nanostructure-initiator mass spectrometry-based assays for analysis glycosyl hydrolase most recently an assay modifying enzymes. Here integrate these two into a single multiplexed against...