A5001915717- Cassava research and cyanide
- Botanical Research and Applications
- Isotope Analysis in Ecology
- Polysaccharides and Plant Cell Walls
- Analytical Chemistry and Chromatography
- Enzyme Catalysis and Immobilization
- Advanced Cellulose Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Protein Hydrolysis and Bioactive Peptides
- Light effects on plants
- Microbial bioremediation and biosurfactants
- Crop Yield and Soil Fertility
- Plant Molecular Biology Research
- Biological Control of Invasive Species
- Plant Gene Expression Analysis
- Peptidase Inhibition and Analysis
- Traditional and Medicinal Uses of Annonaceae
- Oil Palm Production and Sustainability
- Plant Parasitism and Resistance
- Plant Growth Enhancement Techniques
- Plant responses to water stress
- Enzyme Structure and Function
- Plant Micronutrient Interactions and Effects
- Polyamine Metabolism and Applications
- Plant tissue culture and regeneration
James Hutton Institute
2024
University of Edinburgh
2020-2022
University of Copenhagen
2014-2021
Laboratoire de Biochimie
2016
Planta
2016
Czech Academy of Sciences
2012-2013
Czech Academy of Sciences, Institute of Experimental Botany
2013
Czech Academy of Sciences, Institute of Microbiology
2012
Cyanogenic glycosides are phytoanticipins involved in plant defence against herbivores by virtue of their ability to release toxic hydrogen cyanide (HCN) upon tissue disruption. In addition, endogenous turnover cyanogenic without the liberation HCN may offer plants an important source reduced nitrogen at specific developmental stages. To investigate presence putative products glycosides, comparative metabolic profiling using LC–MS/MS and high resolution MS (HR–MS) complemented ion-mobility...
Abstract Background The expression of genes in Corynebacterium glutamicum , a Gram-positive non-pathogenic bacterium used mainly for the industrial production amino acids, is regulated by seven different sigma factors RNA polymerase, including stress-responsive ECF-sigma factor SigH. sigH gene located cluster together with rshA gene, putatively encoding an anti-sigma factor. aim this study was to analyze transcriptional regulation and effects RshA on SigH regulon, order refine model...
The lack of robust, high-throughput, and sensitive analytical strategies that can conclusively map the structure glycans has significantly hampered progress in fundamental applied aspects glycoscience. Resolution anomeric α/β glycan linkage within oligosaccharides remains a particular challenge. Here, we show "memory" configuration is retained following gas-phase glycosidic bond fragmentation during tandem mass spectrometry (MS2). These findings allow for integration MS2 with ion mobility...
The important cereal crop Sorghum bicolor (L.) Moench biosynthesize and accumulate the defensive compound dhurrin during development. Previous work has suggested multiple roles for including a function as nitrogen storage/buffer. Crucial this is endogenous turnover of which putative pathways have been but not confirmed. In study, biosynthesis in developing sorghum grain was studied by metabolite profiling time-resolved transcriptome analyses. Dhurrin found to early phase development reaching...
Almond and sweet cherry are two economically important species of the Prunus genus. They both produce cyanogenic glucosides prunasin amygdalin. As part a two-component defense system, amygdalin release toxic hydrogen cyanide upon cell disruption. In this study, we investigated potential role some its derivatives in endodormancy these species. The content endogenous turnover products course flower development/opening was examined five almond cultivars – differing from very early to extra-late...
Current cell-wall models assume no covalent bonding between cellulose and hemicelluloses such as xyloglucan or mixed-linkage β-d-glucan (MLG). However, Equisetum hetero-trans-β-glucanase (HTG) grafts onto oligosaccharides (XGOs) - and, we now show, polysaccharide in vitro, thus exhibiting CXE (cellulose:xyloglucan endotransglucosylase) activity. In addition, HTG also catalyzes MLG-to-XGO (MXE activity). this study, explored the action of native plant cell walls tested whether expansin...
Abstract Lima bean, Phaseolus lunatus , is a crop legume that produces the cyanogenic glucosides linamarin and lotaustralin. In legumes Lotus japonicus Trifolium repens biosynthesis of these two α‐hydroxynitrile involves cytochrome P450 enzymes CYP79 CYP736 families UDP‐glucosyltransferase. Here, we identify CYP79D71 as first enzyme pathway in P. producing oximes from valine isoleucine. A second family member, CYP79D72, was shown to catalyze formation leucine‐derived oximes, which act...
Abstract Introduction Recent advances in high-throughput methodologies the ‘omics’ and synthetic biology fields call for rapid sensitive workflows metabolic phenotyping of complex biological samples. Objective The objective this research was to evaluate a straightforward implement LC–MS metabolomics method using commercially available chromatography column that provides increased throughput. Reducing run time can potentially impact therefore effects ion mobility spectrometry expand peak...
Abstract Auxin is a key plant regulatory molecule, which acts upon plethora of cellular processes, including those related to cell differentiation and elongation. Despite the stunning progress in all disciplines auxin research, mechanisms auxin-mediated rapid promotion expansion underlying rearrangement wall components are poorly understood. This partly due limitations current methodologies for probing auxin. Here we describe click chemistry-based approach, using an azido derivative...
With the growing global population and climate change, achieving food security is a pressing challenge. Vertical farming has potential to support local production security. As Total Controlled Environment Agriculture (TCEA) system, vertical employs LED lighting which offers opportunities modulate light spectrum intensity, thus can be used influence plant growth phytochemical composition, including antioxidants beneficial for human health. In this study, we investigated effect of four...
Certain transglucanases can covalently graft cellulose and mixed-linkage β-glucan (MLG) as donor substrates onto xyloglucan acceptor substrate thus exhibit cellulose:xyloglucan endotransglucosylase (CXE) MLG:xyloglucan (MXE) activities in vivo vitro. However, missing information on factors that stimulate or inhibit these hetero-transglucosylation reactions limits our insight into their biological functions. To explore influence hetero-transglucosylation, we studied Equisetum fluviatile...
Isoflavone synthase (IFS; CYP93C) plays a key role in the biosynthesis of phenolic secondary metabolites, isoflavonoids. These compounds, which are well-known for their benefits to human health and plant defence, produced mostly legumes. However, more than 200 them have been described 59 other families without any knowledge respective IFS orthologue genes (with sole exception sugar beet). In this study, we selected from Pisum sativum L. (CYP93C18) functional expression. CYP93C18 was...
Abstract Plant natural products encompass an enormous chemical diversity bearing witness to great molecular innovation that occured throughout land plant evolution. Cytochrome P450 monooxygenases (CYPs) catalyze a wide variety of monooxygenation reactions essential the metabolic repertoire plants products. Ferns constitute second largest group vascular and hold significant phylogenetic position in evolution, lying sister seed plants. To date, CYP has not been described for this taxon pathway...
With the growing global population and climate change, achieving food security is a pressing challenge (1) . Vertical farming has potential to support local production security. In UK females younger adults appear be particularly vulnerable micronutrient shortfalls from sources alone. Levels of intakes including zinc iron are below recommended daily intake (2) As Total Controlled Environment Agriculture (TCEA) system, vertical employs hydroponics using nutrient solution which offers...
Almond (Prunus dulcis L.) and cassava (Manihot esculenta Crantz) are two important food crops representatives of more than 2600 plants species known to produce amino acid-derived cyanogenic glucosides. Cyanogenic glucosides function as effective phytoanticipins, responsible for a sophisticated defence mechanism: the release toxic HCN (cyanogenesis) upon tissue disruption [1]. However, not only stagnant compounds. Increasing evidence points towards dynamic endogenous turnover provide plant...