A5101993907- Pharmacogenetics and Drug Metabolism
- Genomics, phytochemicals, and oxidative stress
- Plant Diversity and Evolution
- Cassava research and cyanide
- Plant biochemistry and biosynthesis
- Nanoparticle-Based Drug Delivery
- Botany and Plant Ecology Studies
- Microbial Metabolic Engineering and Bioproduction
- Essential Oils and Antimicrobial Activity
- Mediterranean and Iberian flora and fauna
- Plant Ecology and Taxonomy Studies
- Lipid Membrane Structure and Behavior
- Botany, Ecology, and Taxonomy Studies
- Moringa oleifera research and applications
- Biological Control of Invasive Species
- Oral and gingival health research
- Oral Health Pathology and Treatment
- Plant and Fungal Species Descriptions
- Plant Taxonomy and Phylogenetics
- Plant Parasitism and Resistance
- RNA Interference and Gene Delivery
- Plant Gene Expression Analysis
- Receptor Mechanisms and Signaling
- Photosynthetic Processes and Mechanisms
- Computational Drug Discovery Methods
University of Copenhagen
1981-2024
Concordia University
2018
University of Alberta
1990-1991
Natural History Museum Aarhus
1987
Metabolic control is mediated by the dynamic assemblies and function of multiple redox enzymes. A key element in these assemblies, P450 oxidoreductase (POR), donates electrons selectively activates numerous (>50 humans >300 plants) cytochromes (CYPs) controlling metabolism drugs, steroids xenobiotics natural product biosynthesis plants. The mechanisms underlying POR-mediated CYP remain poorly understood to date no ligand binding has been described regulate specificity POR. Here, using a...
Indole glucosinolates (IGs) are plant secondary metabolites that derived from the amino acid tryptophan. The product of Arabidopsis (Arabidopsis thaliana) IG core biosynthesis, indol-3-ylmethyl glucosinolate (I3M), can be modified by hydroxylation and subsequent methoxylation indole ring in position 1 (1-IG modification) or 4 (4-IG modification). Products 4-IG modification pathway mediate plant-enemy interactions particularly important for innate immunity. While CYP81Fs encoding cytochrome...
Cyanogenic glucosides are a class of specialized metabolites widespread in the plant kingdom. α-hydroxynitriles, and their hydrolysis releases toxic hydrogen cyanide, providing an effective chemical defense against herbivores. Eucalyptus cladocalyx is cyanogenic tree, allocating up to 20% leaf nitrogen biosynthesis monoglucoside, prunasin. Here, mass spectrometry analyses E. tissues revealed spatial ontogenetic variations prunasin content, as well presence diglucoside amygdalin flower buds...
Biosynthesis of steviol glycosides in planta proceeds via two cytochrome P450 enzymes (CYPs): kaurene oxidase (KO) and kaurenoic acid hydroxylase (KAH). KO KAH function succession with the support a NADPH-dependent reductase (CPR) to convert steviol. This work describes platform for recombinant production glucosides (SGs) Saccharomyces cerevisiae, demonstrating full reconstituted pathway from simple sugar glucose SG precursor With focus on optimization KO-KAH activities, combinations...
O transtorno conversivo, também denominado de sintomas neurológicos funcionais, caracteriza-se por manifestações clínicas disfunções neurológicas na ausência uma base orgânica identificável. Entre os sintomas, a perda da articulação fala, representa um desafio diagnóstico e terapêutico, exigindo abordagem multidisciplinar. Este trabalho apresenta relato caso paciente com dislalia conversiva, correlacionando literatura atual. A revisão discute neurofisiologia do transtorno, critérios...
(Molecular Plant 14(8); 1244–1265; August 2 2021; https://doi.org/10.1016/j.molp.2021.06.028) In the original supplemental data 1 of this article, there is an error in column that reports presence CYP71 family members plants. The cell lists plant taxons where are found should only show ‘A’ instead ‘A, G’, as CYP realized to be present angiosperms. Figure 2, which illustrates some information from 1, correctly shows authors apologize for and any inconvenience may have caused. cytochrome P450...
Cyanogenic glucosides are important defense molecules in plants with useful biological activities animals. Their last biosynthetic step consists of a glycosylation reaction that confers stability and increases structural diversity is catalyzed by the UDP-dependent glycosyltransferases (UGTs) glycosyltransferase family 1. These versatile enzymes have large varied substrate scopes, structure-function relationships controlling scope specificity remain poorly understood. Here, we report...
Azole-resistant Aspergillus fumigatus (ARAf) fungi have been found inconsistently in the environment Denmark since 2010. During 2018-2020, nationwide surveillance of clinical A. reported environmental TR
The oral cavity is constantly lubricated by saliva and even small amounts of xenobiotics / or their metabolites in the may affect mucosa. Our aim was therefore to clarify if xenobiotic metabolizing enzymes CYP1A2 CYP3A4 are expressed salivary glands.Formalin-fixed paraffin-embedded specimens from parotid (10), submandibular (7) labial (10) glands were examined immunohistochemically situ hybridization for expression protein mRNA.CYP1A2 mRNA detected ductal seromucous serous acinar cells all...
Abstract Plant metabolism depends on cascade reactions mediated by dynamic enzyme assemblies known as metabolons. In this context, the cytochrome P450 (P450) superfamily catalyze key underpinning unique diversity of bioactive compounds. contrast to their soluble bacterial counterparts, eukaryotic P450s are anchored endoplasmic reticulum membrane and serve metabolon nucleation sites. Hence, anchoring appears play a pivotal role in evolution complex biosynthetic pathways. Here, model assay...
Summary The economic and ecologically important genus Eucalyptus is rich in structurally diverse specialized metabolites. While some metabolite classes are highly prevalent across the genus, cyanogenic glucoside prunasin only produced by c. 3% of species. To investigate evolutionary mechanisms behind biosynthesis , we compared de novo assembled transcriptomes, together with online resources between acyanogenic Identified genes were characterized vivo vitro . Pathway characterization camphora...
Background: Oral lichen planus (OLP) is a chronic mucosal disease with characteristic clinical phenotype. Environmental exposures, e.g. drugs have been associated the pathogenesis. Objectives: To test hypothesis that some OLP lesions pharmacological pathogenesis related to polymorphisms of cytochrome P450 enzymes (CYPs) resulting in poor or intermediate CYP metabolism. Methods: One hundred and twenty patients 180 gender‐matched controls without were genotyped for CYP2C9 , CYP2C19 CYP2D6...
Lichenoid drug eruptions (LDE) in the oral cavity are adverse reactions (ADR) that impossible to differentiate from lichen planus (OLP) as no phenotypic criteria exist. Impaired function of polymorphic cytochrome 450-enzymes (CYPs) may cause increased plasma concentration some drugs resulting ADR/LDE. In an earlier study we did not find more patients with OLP (OLPs) impaired CYP-genotype.To test if OLPs have CYP-phenotype than be expected CYP-genotype and clinical characterising LDE.One...
Abstract Plant natural products encompass an enormous chemical diversity bearing witness to great molecular innovation that occured throughout land plant evolution. Cytochrome P450 monooxygenases (CYPs) catalyze a wide variety of monooxygenation reactions essential the metabolic repertoire plants products. Ferns constitute second largest group vascular and hold significant phylogenetic position in evolution, lying sister seed plants. To date, CYP has not been described for this taxon pathway...
Alzheimer's disease (AD) is characterized by complex interactions between neuropathological markers, metabolic dysregulation, and structural brain changes. In this study, we utilized a multimodal approach, combining immunohistochemistry, functional mapping, microstructure sensitive diffusion MRI (dMRI) to progressively investigate these in the 5xFAD mouse model of AD. Our analysis revealed age-dependent region-specific accumulation key AD including amyloid-beta (Aβ), GFAP, IBA1, with...
The new genus Aschistanthera and its only species, cristanthera , are described. Characteristics additional to the diagnosis emphasized parts of flowers fruits distribution illustrated.