A5026718450- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- Enzyme Structure and Function
- Complement system in diseases
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Polysaccharides and Plant Cell Walls
- Infant Nutrition and Health
- Toxin Mechanisms and Immunotoxins
- Gut microbiota and health
- Seaweed-derived Bioactive Compounds
- Diet, Metabolism, and Disease
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Machine Learning in Bioinformatics
- Monoclonal and Polyclonal Antibodies Research
- Amino Acid Enzymes and Metabolism
- Enzyme function and inhibition
- Polyamine Metabolism and Applications
- Biochemical and Molecular Research
- Food composition and properties
Novo Nordisk Foundation
2019-2025
Technical University of Denmark
2012-2025
Novo Nordisk (Denmark)
2024
University of Copenhagen
2016-2021
Lund University
2012-2016
MAX IV Laboratory
2013-2016
Aarhus University
2006-2010
MRC Laboratory of Molecular Biology
2009
Oxidative plant cell-wall processing enzymes are of great importance in biology and biotechnology. Yet, our insight into the functional interplay amongst such oxidative remains limited. Here, a phylogenetic analysis auxiliary activity 7 family (AA7), currently harbouring oligosaccharide flavo-oxidases, reveals striking abundance AA7-genes phytopathogenic fungi Oomycetes. Expression five fungal enzymes, including three from unexplored clades, expands AA7-substrate range unveils...
Glycosyltransferases (GTs) catalyze the formation of glycosidic bonds in carbohydrates and glycoconjugates, with various outcomes depending not only on acceptor molecules they bind but also type bond form (C–O, C–N, C–S, or C–C). Here we show that glucosyltransferase UGT1 from indigo plant Polygonum tinctorium catalyzes either N-, O-, S-glycosylation similar rates. We solve structure enzyme complex its donor substrates elucidate molecular basis S-specificities using experimental mutagenesis...
The exon junction complex (EJC) is deposited onto spliced mRNAs and involved in many aspects of mRNA function. We have recently reconstituted solved the crystal structure EJC core made MAGOH, Y14, most conserved portion MLN51, DEAD-box ATPase eIF4AIII bound to RNA presence an ATP analog. heterodimer MAGOH/Y14 inhibits turnover by eIF4AIII, thereby trapping RNA, but exact mechanism behind this remains unclear. Here, we present ADP-AIF 3 , first a helicase transition-mimicking state during...
Staphylococcus aureus secretes the SSL7 protein as part of its immune evasion strategy. The binds both complement C5 and IgA, yet it is unclear whether cross-links these two proteins and, if so, what purpose this serves pathogen. We have isolated a stable IgA-SSL7-C5 complex, our crystal structure C5-SSL7 complex confirms that binding to occurs exclusively through C-terminal beta-grasp domain leaving OB free interact with IgA. interacts >70 A from C5a cleavage site without inducing...
Natural product <italic>C</italic>-glycosyltransferases are forming the rare <italic>C</italic>-glycosidic bonds that represent a synthetic challenge.
Summary Glycan utilization plays a key role in modulating the composition of gut microbiota, but molecular insight into oligosaccharide uptake by this microbial community is lacking. Arabinoxylo‐oligosaccharides ( AXOS ) are abundant diet, and selectively fermented probiotic bifidobacteria colon. Here we show how selectivity for established strain B ifidobacterium animalis subsp. lactis Bl ‐04. The binding protein AXBP , which associated with an ATP ‐binding cassette ABC transporter that...
Isomaltooligosaccharides (IMO) have been suggested as promising prebiotics that stimulate the growth of probiotic bacteria. Genomes lactobacilli from acidophilus group, represented by Lactobacillus NCFM, encode α-1,6 glucosidases family GH13_31 (glycoside hydrolase 13 subfamily 31) confer degradation IMO. These genes reside frequently within maltooligosaccharide utilization operons, which include an ATP-binding cassette transporter and α-glucan active enzymes, e.g., maltogenic amylases...
Abstract Blue denim, a billion-dollar industry, is currently dyed with indigo in an unsustainable process requiring harsh reducing and alkaline chemicals. Forming directly the yarn through indican (indoxyl-β-glucoside) promising alternative route mild conditions. Indican eliminates requirement for agent while still ending as indigo, only known molecule yielding unique hue of blue denim. However, bulk source missing. Here, we employ enzyme engineering guided by techno-economic analyses to...
The Bifidobacterium genus harbours several health promoting members of the gut microbiota. Bifidobacteria display metabolic specialization by preferentially utilizing dietary or host-derived β-galactosides. This study investigates biochemistry and structure a glycoside hydrolase family 42 (GH42) β-galactosidase from probiotic animalis subsp. lactis Bl-04 (BlGal42A). BlGal42A displays preference for undecorated β1-6 β1-3 linked galactosides populates phylogenetic cluster with close...
Alginate lyases (ALs) catalyze the depolymerization of brown macroalgae alginates, widely used naturally occurring polysaccharides. Their molecular reaction mechanism remains elusive due to lack catalytically competent Michaelis-Menten-like complex structures. Here, we provide structural snapshots and dissect mannuronan-specific ALs from family 7 polysaccharide (PL7), employing time-resolved NMR, X-ray, neutron crystallography, QM/MM simulations. We reveal protonation state critical active...
The macromolecular crystallography beamline I911-3, part of the Cassiopeia/I911 suite beamlines, is based on a superconducting wiggler at MAX II ring IV Laboratory in Lund, Sweden. energy-tunable within range between 6 and 18 keV. I911-3 opened for users 2005. In 2010-2011 experimental station was completely rebuilt refurbished such that it has become state-of-the-art with better possibilities rapid throughput, crystal screening work smaller samples. This paper describes complete how...
Regioselective glycosylation is a chemical challenge, leading to multistep syntheses with protecting group manipulations, ultimately resulting in poor atom economy and compromised sustainability. Enzymes allow eco-friendly regioselective bond formation fully deprotected substrates single reaction. For the selective glucosylation of silibinin, pharmaceutical challenged low solubility, enzyme engineering has previously been employed, but yields kcat were limited, prohibiting application...
Feruloyl esterases (EC 3.1.1.73), belonging to carbohydrate esterase family 1 (CE1), hydrolyze ester bonds between ferulic acid (FA) and arabinose moieties in arabinoxylans. Recently, some CE1 enzymes identified metagenomics studies have been predicted contain a 48 carbohydrate-binding module (CBM48), CBM associated with starch binding. Two of these CE1s, wastewater treatment sludge (wts) Fae1A wtsFae1B isolated from surplus sludge, cognate CBM48 domain are feruloyl esterases, wtsFae1A binds...
Sucrose phosphorylases, through transglycosylation reactions, are interesting enzymes that can transfer regioselectively glucose from sucrose, the donor substrate, onto acceptors like flavonoids to form glycoconjugates and hence modulate their solubility bioactivity. Here, we report for first time structure of sucrose phosphorylase marine bacteria Alteromonas mediterranea (AmSP) its enzymatic properties. Kinetics hydrolysis transglucosylation capacities on (+)-catechin were investigated....
Alginate is a polysaccharide consumed by humans in edible seaweed and different foods where it applied as texturizing hydrocolloid or encapsulations of drugs probiotics. While gut bacteria are found to utilize ferment alginate health-beneficial short-chain fatty acids, knowledge on the details molecular reactions sparse. Alginates composed mannuronic acid (M) its C-5 epimer guluronic (G). An alginate-related utilization locus (PUL) has been identified bacterium Bacteroides eggerthii DSM...
In recent years, a plethora of studies have demonstrated the paramount physiological importance gut microbiota on various aspects human health and development. Particular focus has been set probiotic members this community, best studied which are assigned into Lactobacillus Bifidobacterium genera. Effects such as pathogen exclusion, alleviation inflammation allergies, colon cancer, other bowel disorders attributed to activity bacteria, selectively ferment prebiotics comprising mainly...
Enzymes active on glycosidic bonds are defined according to the stereochemistry of both substrates and products reactions they catalyze. The CAZy classification further assigns these enzymes into sequence-based families sharing a common for (either α- or β-) (i.e., inverting retaining mechanism). Here we describe N-acetylgalactosaminidases AmGH109A AmGH109B GH109: glycoside hydrolase family 109) from human gut symbiont Akkermansia muciniphila. Notably, displays α-retaining β-inverting...
Cyanogenic glucosides are important defense molecules in plants with useful biological activities animals. Their last biosynthetic step consists of a glycosylation reaction that confers stability and increases structural diversity is catalyzed by the UDP-dependent glycosyltransferases (UGTs) glycosyltransferase family 1. These versatile enzymes have large varied substrate scopes, structure-function relationships controlling scope specificity remain poorly understood. Here, we report...