A5037513499- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Plant and Fungal Interactions Research
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Agriculture and Farm Safety
- Insect symbiosis and bacterial influences
- Toxoplasma gondii Research Studies
- Transgenic Plants and Applications
- Aquaculture disease management and microbiota
- Virology and Viral Diseases
- interferon and immune responses
- Insect Pest Control Strategies
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Vector-borne infectious diseases
- Bacteriophages and microbial interactions
- Cholinesterase and Neurodegenerative Diseases
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2019-2024
NeuroDiderot
2020-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019-2024
École Nationale Vétérinaire d'Alfort
2019-2024
Paris-Est Sup
2020
The Pirbright Institute
2007-2015
Vector & Vector-Borne Diseases Research Institute
2014
Département Santé Animale
2011
Institut de Neurobiologie de la Méditerranée
2002-2005
Laboratoire de Virologie Moléculaire et Structurale
2005
Sedoreoviridae is a large family of icosahedral viruses that are usually regarded as non-enveloped with segmented (10–12 linear segments) dsRNA genomes 18–26 kbp. Sedoreovirids have broad host range, infecting mammals, birds, crustaceans, arthropods, algae and plants. Some them important pathogenic potential for humans (e.g. rotavirus A), livestock bluetongue virus) plants rice dwarf virus). This summary the ICTV Report on , which available at ictv.global/report/sedoreoviridae .
Full-length and partial genome sequences of four members the genus Aquareovirus , family Reoviridae (Golden shiner reovirus, Grass carp Striped bass reovirus golden ide reovirus) were characterized. Based on sequence comparison, unclassified was shown to be a member species C . The status another aquareovirus, also examined. Sequence analysis showed that it did not belong A or but assessment its relationship B D E F hampered by absence genetic data from these species. In agreement with...
The genus Orbivirus includes both insect and tick-borne viruses. orbivirus genome, composed of 10 segments dsRNA, encodes 7 structural proteins (VP1–VP7) 3 non-structural (NS1–NS3). An open reading frame (ORF) that spans almost the entire length genome segment-9 (Seg-9) VP6 (the viral helicase). However, bioinformatic analysis recently identified an overlapping ORF (ORFX) in Seg-9. We show ORFX a new protein, here as NS4. Western blotting confocal fluorescence microscopy, using antibodies...
Spinareoviridae is a large family of icosahedral viruses that are usually regarded as non-enveloped with segmented (9-12 linear segments) dsRNA genomes 23-29 kbp. Spinareovirids have broad host range, infecting animals, fungi and plants. Some important pathogenic potential for humans (e.g. Colorado tick fever virus), livestock avian orthoreoviruses), fish aquareoviruses) plants rice ragged stunt virus black streaked dwarf virus). This summary the ICTV Report on Spinareoviridae, which...
During an investigation of arboviruses in China, a novel dsRNA virus was isolated from adult female Armigeres subalbatus. Full genome sequence analysis showed the to be related members family Totiviridae, and therefore named ‘Armigeres subalbatus totivirus’ (AsTV). Transmission electron microscopy identified icosahedral, non-enveloped particles with mean diameter 40 nm. The AsTV is 7510 bp length, two ORFs. ORF1 (4443 nt) encodes coat-protein dsRNA-binding domain (which may involved evasion...
An orbivirus designated Yunnan (YUOV) was isolated from Culex tritaeniorhynchus mosquitoes collected in the province of China. Electron microscopy showed particles with typical morphology. The YUOV genome sequenced completely and compared previously characterized genomes. Significant identity scores were detected between proteins encoded by segments (Seg-1 to Seg-10) those their homologues insect-borne tick-borne orbiviruses. Analysis VP1 (Pol) VP2 (T2, which correlates virus serogroup)...
The complete nucleotide sequence of Great Island virus (GIV) genome was determined, along with segments (Seg) 1, 2 and 6 Kemerovo (KEMV), Lipovnik (LIPV) Tribec (TRBV) viruses. All four viruses, together Broadhaven virus, are currently classified within the species have been isolated from ticks, birds or humans. Sequence comparisons showed that Seg-4 GIV encoded outer-capsid protein responsible for cell attachment, although it approximately half length its counterpart in Culicoides...
Banna virus (BAV) is the type species of genus Seadornavirus within family Reoviridae. The Chinese BAV isolate (BAV-Ch), which causes encephalitis in humans, was shown to have a structural organization and particle morphology reminiscent that rotaviruses, with fibre proteins projecting from surface particle. Intact BAV-Ch particles contain seven proteins, two (VP4 VP9) form outer coat. inner (core) five additional (VP1, VP2, VP3, VP8 VP10) are 'non-turreted', relatively smooth appearance....
The complete genomes of Orungo virus (ORUV), Lebombo (LEBV) and Changuinola (CGLV) were sequenced, confirming that they each encode 11 distinct proteins (VP1-VP7 NS1-NS4). Phylogenetic analyses cell-attachment protein 'outer-capsid 1′ (OC1), show orbiviruses fall into three large groups, identified as: VP2(OC1), in which OC1 is the 2nd largest protein, including Culicoides transmitted orbiviruses; VP3(OC1), includes mosquito VP4(OC1) tick viruses. Differences size between these places T2...
Many novel emerging orbiviruses have been isolated in the past 15 years. Important viruses include Peruvian horse sickness virus (PHSV) and Yunnan orbivirus (YUOV), pathogens of equids which were originally almost simultaneously from 1997 to 1999 People's Republic China, Australia Peru. YUOV has also cattle, sheep a dog. The isolation dog is not first case an being carnivore. Bluetongue African earlier detected carnivores fed on contaminated meat. PHSV both offer opportunity study emergence...
Bioinformatic analyses have predicted that orbiviruses encode an additional, small non-structural protein (NS5) from a secondary open reading frame on genome segment 10. However, this has not previously been detected in infected mammalian or insect cells. NS5-specific antibodies were generated mice and used to identify NS5 synthesised orbivirus-infected BSR cells transfected with expression plasmids. Confocal microscopy shows although accumulates the nucleus, particularly nucleolus, which...
BTV-4 structural proteins VP2 (as two domains: VP2D1 and VP2D2), VP5 (lacking the first 100 amino acids: VP5Δ1-100) full-length VP7, expressed in bacteria as soluble glutathione S-transferase (GST) fusion-proteins, were used to immunise Balb/c α/β interferon receptor knock-out (IFNAR(-/-)) mice. Neutralising antibody (NAbs) titres (expressed log10 of reciprocal last dilution mouse serum which reduced plaque number by ≥50%) induced domains ranged from 1.806 2.408 IFNAR(-/-) The immunised mice...
Vaccinia virus (Orthopoxvirus) F17 protein is a major virion structural phosphoprotein having molecular weight of 11 kDa. Recently, it was shown that synthesised in infected cells interacts with mTOR subunits to evade cell immunity and stimulate late viral synthesis. Several years back, we purified an kDa inhibited synthesis reticulocyte lysate from virions, possesses all physico-chemical properties protein. To investigate this discrepancy, used defective vaccinia particles devoid the...
Bluetongue is an economically important disease of domesticated and wild ruminants caused by bluetongue virus (BTV). There are at least 36 different serotypes BTV (the identity which determined its outer-capsid protein VP2), most transmitted Culicoides biting midges. IFNAR(-/-) mice immunised with plant-expressed VP2 (rVP2) -1, -4 or -8, the smaller rVP5 BTV-10, mock-immunised PBS, were subsequently challenged virulent strains BTV-4 BTV-8, attenuated clone BTV-1 (BTV-1RGC7). The that had...
Bluetongue is a severe, economically important disease of ruminants that widely distributed in tropical and temperate regions around the world. It associated with major production losses, restrictions animal movements trade, as well costs developing implementing effective surveillance control measures. Mammalian hosts infected bluetongue virus (BTV) generate protective neutralising antibody response targeting BTV outer-capsid protein serotype-specific antigen, VP2. VP2 proteins have been...
ABSTRACT VP6, VP7, VP9, VP10, VP11, and VP12 of Colorado tick fever virus (CTF virus), a member the genus Coltivirus , family Reoviridae were expressed in bacteria with pGEX-4T-2 vector. A partial sequence VP7 (designated pVP7) was chosen to elaborate an enzyme-linked immunosorbent assay (ELISA) for detecting anti-CTF immunoglobulin G (IgG) antibodies humans. This based on two observations: (i) among all proteins, pVP7 showed highest immunoreactivity hyperimmune ascitic fluid; (ii) provide...
The complete nucleotide sequence of Middelburg virus (MIDV) was determined for strain MIDV-857 from Zimbabwe. isolation this in 1993 a horse that died showing severe clinical signs represents the first indication MIDV can cause disease equids. Full-length cDNA copies viral genome were successfully synthesized by an innovative RT-PCR amplification approach using ‘anchor primer’ combined with SMART methodology described previously synthesis full-length segments dsRNA viruses. is 11 674 nt,...
Liao ning virus (LNV) is related to Banna virus, a known human-pathogen present in south-east Asia. Both viruses belong the genus Seadornavirus, family Reoviridae. LNV causes lethal haemorrhage experimentally infected mice. Twenty seven isolates of were made from mosquitoes collected different locations within Xinjiang province north-western China during 2005. These caught accommodation human patients with febrile manifestations, or animal barns where sheep represent main livestock species....
Banna virus (BAV) particles contain seven structural proteins: VP4 and VP9 form an outer-capsid layer, whilst the core contains three major proteins (VP2, VP8 VP10) two minor (VP1 VP3). Sequence analysis showed that VP3 motifs [Kx(I/V/L)S] (Hx(n)H) have previously been identified in guanylyltransferases of other reoviruses. Incubation purified BAV-Ch with [alpha-32P]GTP resulted exclusive covalent labelling VP3, demonstrating autoguanylation activity (which is considered indicative...