A5101443447- Immune Cell Function and Interaction
- Social Policies and Family
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Social Sciences and Governance
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS Research and Interventions
- Myasthenia Gravis and Thymoma
- COVID-19 Clinical Research Studies
- Psychoanalysis and Psychopathology Research
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- Hematopoietic Stem Cell Transplantation
- Mast cells and histamine
- Aging, Elder Care, and Social Issues
- Cardiac Fibrosis and Remodeling
- Cytomegalovirus and herpesvirus research
- Renal Transplantation Outcomes and Treatments
- Viral Infections and Vectors
- Reproductive System and Pregnancy
- Inflammatory Myopathies and Dermatomyositis
- Chronic Lymphocytic Leukemia Research
Université Paris Cité
2021-2023
Institut Pasteur
2023
Theravectys (France)
2023
Sorbonne Université
2009-2021
Institut Pierre Louis d‘Épidémiologie et de Santé Publique
2016-2021
Pitié-Salpêtrière Hospital
1988-2021
Inserm
2004-2021
Production et analyse de données en sciences de la vie et en santé
2018
Fondation pour l’innovation en Cadiométabolisme et Nutrition
2017
Université Sorbonne Paris Nord
2017
Interferons interfere with lung repair (IFNs) are central to antiviral immunity. Viral recognition elicits IFN production, which in turn triggers the transcription of IFN-stimulated genes (ISGs), engage various functions. Type I IFNs (IFN-α and IFN-β) widely expressed can result immunopathology during viral infections. By contrast, type III (IFN-λ) responses primarily restricted mucosal surfaces thought confer protection without driving damaging proinflammatory responses. Accordingly, IFN-λ...
Background. The stable immune control of human immunodeficiency virus (HIV) in long-term nonprogressors (LTNPs) with protective leukocyte antigen (HLA) alleles raises the question whether and how these influence distribution HIV reservoirs. Methods. Cell-associated HIV-DNA levels were quantified blood sorted resting CD4 T-cell subsets from 8 LTNPs 10 without HLA-B*27 or HLA-B*57 (HLA-B27/B57). Results. A remarkably lower infection level central memory T cells (TCM) was an exclusive feature...
Abstract Background Therapeutic control of HIV replication reduces the size viral reservoir, particularly among central memory CD4+ T cells, and this effect might be accentuated by early treatment. Methods We examined ART initiated at time primary infection (early ART), lasting 2 6 years in 11 10 patients, respectively, on reservoir peripheral resting sorted into naive (TN), (TCM), transitional (TTM) effector (TEM) comparison with post-treatment controllers (PTCs). Results Between baseline...
We report a double-site enzyme-linked lactate dehydrogenase immunodetection assay (DELI), highly sensitive antigen-capture immunosorbent assay, which proved to be more for the detection of Plasmodium falciparum than thick blood smears, as polymerase chain reaction, and probably reliable. This technique can help detect infra-microscopic parasitemias (one parasite in 10(6)-10(8) red cells) from biological samples, being quantitative, provide fast substitute smears epidemiologic purposes. The...
Optimizing therapeutic strategies for an HIV cure requires better understanding the characteristics of early HIV-1 spread among resting CD4+ cells within first month primary infection (PHI). We studied immune distribution, diversity, and inducibility total HIV-DNA following cell subsets: monocytes, peripheral blood activated CD4 T cells, long-lived (naive [TN] central-memory [TCM]) short-lived (transitional-memory [TTM] effector-memory [TEM]) CD4+T from 12 acutely-infected individuals...
Immune checkpoint inhibitors for melanoma improve adaptive T cell immunity during COVID-19 without exacerbating inflammation.
Reducing drug burden is a key challenge for achieving lifelong suppressive HIV therapy. Dolutegravir, with high potency, long half-life and genetic barrier, offers potential monotherapy. This observational single-centre study enrolled all patients RNA (viral load) <50 copies/mL at least 12 months, CD4 >350 cells/mm3 no failure under integrase inhibitor therapy who had switched from ART to dolutegravir monotherapy (50 mg/day). Primary outcome was proportion of viral load week 24. Twenty-eight...
Abstract There is currently no therapy to limit the development of cardiac fibrosis and consequent heart failure. We have recently shown that post-myocardial infarction (MI) can be regulated by resident cells with a fibrogenic signature identified expression PW1 ( Peg3 ). Here we identify αV-integrin (CD51) as an essential regulator + behavior. used transcriptomic proteomic approaches specific cell-surface markers for found was expressed in almost all (93% ± 1%), predominantly αVβ1 complex....
Objective: To characterize the immune changes after treatment of acute HIV-1 infection with triple nucleoside analogue therapy. Design: Immunological and virological parameters were monitored from day 0 to weeks 36-44 in eight patients [median CD4 cells=451 cells/ml (range: 149-624), viral load=4.8 log10 copies/ml 6.5-3.3)] who started at time primary HIV (PHI) a therapy including zidovudine (ZDV), didanosine (ddI), lamivudine (3TC). Methods: Lymphoid subsets evaluated on peripheral blood...
A superior capacity of controlling HIV has been attributed to CD8(+) T cells directed against HIV-Gag compared Nef, particularly in the context some protective human leukocyte antigen (HLA) alleles. To further elucidate this effect, we multifunctional and differentiation characteristics specific for Nef HLA-B57/5801-positive negative nonprogressors.A head-to-head comparison frequencies, cytokine production was conducted, 11 HLA-B57/5801 HIV-infected individuals selected from a cohort 53...
Mass cytometry is a powerful tool that allows simultaneous analysis of more than 37 markers at the single cell level. particular interest in identification wide variety phenotypes autoimmune diseases. Moreover, cells can be labelled with palladium isotopes and pooled before staining (barcoding). Nevertheless, immunologists often face an important problem concerning choice to included panel. This arises due incompatibility different buffers used for fixation permeabilization various surface...
Abstract Understanding both the role of tumor Ag in CD8 cell differentiation and reasons that cells may work inefficiently is crucial for therapeutic approaches cancer. We studied OT-1 responses vivo a differential Ag-distribution model used EG-7, EL-4 thymoma transfected with OVA. On their initial encounter, underwent programmed expansion lymph nodes, where they acquired ability to migrate encapsulated site after ≥4 divisions, without continuous antigenic stimulation. This short stimulation...
Myasthenia Gravis (MG) is a neurological autoimmune disease characterized by disabling muscle weaknesses due to anti-acetylcholine receptor (AChR) autoantibodies. To gain insight into immune dysregulation underlying early-onset AChR + MG, we performed an in-depth analysis of peripheral mononuclear blood cells (PBMCs) using mass cytometry. PBMCs from 24 MG patients without thymoma and 16 controls were stained with panel 37 antibodies. Using both unsupervised supervised approaches, observed...
Inadequate reconstitution of CD4+ lymphocyte and interleukin (IL)-2 production defect are observed after bone marrow or peripheral blood stem cell transplantation (SCT).We studied immune SCT in 33 consecutive patients who received allogeneic (17 patients) autologous (16 patients). The aims were to assess the regeneration T-cell subset with regard helper differentiation. expansion CD4+CD7- by immunofluorescence analysis. cytokine secretion was analyzed sorting costimulation CD3 CD28 pathways,...
In the neonatal human thymus, early immature precursors co-express CD34 and CD7 cell surface Ags, we have recently shown that its most primitive CD34+7+1- fraction includes TCR-beta-rearranging cells. Bone marrow cord blood also contain a CD34+7+ population. Although this population is heterogeneous in terms of both phenotype differentiation capacities, it may include T cell-committed thymus colonizing (prothymocytes). Recently, has been mouse initiation TCR-beta rearrangements not...