A5061781276- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Microwave Engineering and Waveguides
- Animal Nutrition and Physiology
- Lipid Membrane Structure and Behavior
- Photonic Crystal and Fiber Optics
- Optical Network Technologies
- Bacteriophages and microbial interactions
- Acoustic Wave Resonator Technologies
- Fuel Cells and Related Materials
- RNA and protein synthesis mechanisms
- Coccidia and coccidiosis research
- Membrane Separation Technologies
- Child Nutrition and Water Access
- Advanced MEMS and NEMS Technologies
- Advanced Fiber Optic Sensors
- Supramolecular Chemistry and Complexes
- Radio Frequency Integrated Circuit Design
- Reproductive Physiology in Livestock
- Advanced NMR Techniques and Applications
- Effects of Environmental Stressors on Livestock
- Membrane-based Ion Separation Techniques
- Advanced Antenna and Metasurface Technologies
Indian Institute of Technology Hyderabad
2023-2024
Graphic Era University
2024
University of Illinois Urbana-Champaign
1971-2023
Indian Veterinary Research Institute
2006-2023
Indian Council of Agricultural Research
2023
Indian Institute of Science Bangalore
2014-2019
University of Oklahoma
2015
Jawaharlal Nehru Technological University, Hyderabad
2014
Purdue University West Lafayette
2006-2010
Govind Ballabh Pant University of Agriculture and Technology
2010
In this paper, the authors present a design technique that enables inter-resonator and external coupling control for high-quality-factor ( <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Q</i> ) tunable bandpass filters. The incorporates low- varactors as part of mechanisms without degrading overall high original filter. Detailed methodology equations are presented to illustrate concepts. A first-time demonstration these concepts is widely high-...
The interplay between nucleic acids and lipids underpins several key processes in molecular biology, synthetic biotechnology, vaccine technology, nanomedicine. These interactions are often electrostatic nature, much of their rich phenomenology remains unexplored view the chemical diversity lipids, heterogeneity phases, broad range relevant solvent conditions. Here we unravel zwitterionic lipid membranes DNA nanostructures presence physiologically cations, with purpose identifying new routes...
In the present work, a widely tunable high-Q air filled evanescent cavity bandpass filter is created in an LTCC substrate. A low loss Rogers Duroid <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">reg</sup> flexible substrate forms top of filter, acting as membrane for parasitic capacitor that allows variable frequency loading. commercially available piezoelectric actuator mounted on precise electrical tuning center frequency. The tuned from 2.71...
Abstract DNA nanotechnology allows for the design of programmable DNA-built nanodevices which controllably interact with biological membranes and even mimic function natural membrane proteins. Hydrophobic modifications, covalently linked to DNA, are essential targeted interfacing nanostructures lipid membranes. However, these hydrophobic tags typically induce undesired aggregation eliminating structural control, primary advantage nanotechnology. Here, we study cholesterol-modified using a...
Reported herein is a series of pore-containing polymeric nanotubes based on hydrogen-bonded hydrazide backbone. Nanotubes suitable lengths, possessing hollow cavity about 6.5 Å diameter, mediate highly efficient transport diverse types anions, rather than cations, across lipid membranes. The reported polymer channel, having an average molecular weight 18.2 kDa and 3.6 nm in helical height, exhibits the highest anion-transport activities for iodide (EC50 =0.042 μm or 0.028 mol % relative to...
Reproducing the structure and function of biological membrane channels, synthetic nanopores have been developed for applications in filtration technologies biomolecular sensing. Stable stand-alone created from a variety materials, including peptides, nucleic acids, polymers, solid-state membranes. In contrast to nanopores, however, furnishing such with an atomically defined shape, deliberate placement each every chemical group, remains major challenge. Here, we introduce chemosynthetic...
In this letter, the design and RF packaging of substrate integrated widely tunable filter is presented. The consists two heavily loaded evanescent-mode cavities embedded into substrate. actuated using piezoelectric discs, which move thin, flexible membranes that form top cavities. To demonstrate wide tuning a fabricated measured to cover very frequency range from 0.98 3.48 GHz (tuning ratio-3.55:1). insertion loss less than 3.57 dB for 1.1% fractional bandwidth filter.
Equipping DNA with hydrophobic anchors enables targeted interaction lipid bilayers for applications in biophysics, cell biology, and synthetic biology. Understanding DNA–membrane interactions is crucial rationally designing functional DNA. Here we study the of hydrophobically tagged membranes using a combination experiments atomistic molecular dynamics (MD) simulations. The duplexes are rendered by conjugation to terminal cholesterol anchor or chemical synthesis charge-neutralized...
Here, we report on a novel class of fluorofoldamer-based artificial water channels (AWCs) that combines excellent transport rate and selectivity with structural simplicity robustness. Produced by facile one-pot copolymerization reaction under mild conditions, the best-performing channel (AWC 1) is an n-C8H17-decorated foldamer nanotube average length 2.8 nm pore diameter 5.2 Å. AWC 1 demonstrates ultrafast conduction 1.4 × 1010 H2O/s per channel, outperforming archetypal biological aquaporin...
Nanopore sequencing of nucleic acids has an illustrious history innovations that eventually made commercial nanopore possible. Nevertheless, the present technology leaves much room for improvement, especially with respect to accuracy raw reads and detection nucleotide modifications. Double-nanopore sequencing—an approach where a DNA molecule is pulled back forth by tug-of-war two nanopores—could potentially improve single-molecule read modification offering multiple same fragment. One...
We present a technique to determine the orientation of single fluorophores attached DNA origami structures based on two measurements. First, absorption transition dipole molecule is determined through polarization-resolved excitation measurement. Second, structure obtained from DNA-PAINT nanoscopy Both measurements are performed consecutively fluorescence wide-field microscope. employed this approach study ATTO 647N, 643, and Cy5 covalently 2D rectangular with different nanoenvironments,...
Signal transmission in neurons goes along with changes the transmembrane potential. To report them, different approaches, including optical voltage-sensing dyes and genetically encoded voltage indicators, have evolved. Here, we present a DNA nanotechnology-based system demonstrated its functionality on liposomes. Using origami, incorporated optimized properties such as membrane targeting sensing modularly. As unit, used hydrophobic red dye anchored to an anionic green at connect...
With the advent of nucleosome/nucleotide intercalating drugs, DNA-based nanocarriers have recently gained impetus. However, most newly proposed DNA nanosystems are rather complex, thereby having low scalability and translatability. In this study, we propose a simple nanomatrix core encapsulated within chitosan shell, which is expected to enhance encapsulation efficiency drugs. This has been demonstrated using proflavine hemisulfate (PfHS), model agent that shows improved ROS generation,...
We present an atomistic model of pillared DNA nanotubes (DNTs) and their elastic properties which will facilitate further studies these in several important nanotechnological biological applications. In particular, we introduce a computational design to create 6-helix DNT (6HB) along with its two variants, 6HB flanked symmetrically by double helical pillars (6HB+2) three (6HB+3). Analysis 200 ns all-atom simulation trajectories the presence explicit water ions shows that structures are...
Lipid-anchored DNA can attach functional cargo to bilayer membranes in nanotechnology, synthetic biology, and cell biology research. To optimize anchoring, an understanding of DNA-membrane interactions terms binding strength, extent, structural dynamics is required. Here we use experiments molecular (MD) simulations determine how the membrane cholesterol-modified depends on electrostatic steric factors involving lipid headgroup charge, duplexed or single-stranded DNA, buffer composition. The...
Designed and engineered protein DNA nanopores can be used to sense characterize single molecules control transmembrane transport of molecular species. However, designed biomolecular pores are less than 100 nm in length primarily for across lipid membranes. Nanochannels that span longer distances could as conduits between nonadjacent compartments or cells. Here, we design micrometer-long, 7-nm-diameter nanochannels small traverse according the laws continuum diffusion. Binding origami caps...
In this study, a dual-band lumped-element bandpass filter (DBLEF) is presented, which implemented in low-temperature co-fired ceramic. The design utilizes the tight component coupling possible technology to mimic much more complex network of lumped components. unique that it only single set components create dual-passband response. Detailed equations are presented for design, and DBLEF having passbands at 2.4 5.2 GHz demonstrated, representing common wireless local area bands. This paper...
DNA nanotubes are tubular structures composed of crossover molecules. We present a bottom up approach for the construction and characterization these structures. Various possible topologies constructed such as 6-helix, 8-helix tri-tubes with different sequences lengths. have used fully atomistic molecular dynamics simulations to study structure, stability elasticity Several nanosecond long MD give microscopic details about nanotubes. Based on structural analysis simulation data, we show that...
Rosette nanotubes (RNTs) are a class of materials formed by molecular self-assembly fused guanine–cytosine base (G∧C base). An important feature these self-assembled is their precise atomic structure, intriguing for rational design and optimization as synthetic transmembrane porins. Here, we present experimental observations ion transport across 1.1 nm inner diameter RNT porins (RNTPs) various lengths in the range 5–200 nm. In typical experiment, custom lipophilic RNTPs were first inserted...
Novel vapor-permeable materials are sought after for applications in protective wear, energy generation, and water treatment. Current impermeable effectively block harmful agents but trap heat due to poor vapor transfer. Here we present a new class of materials, permeable dehydrated nanoporous biomimetic membranes (DBMs), based on channel proteins. This application is unexpected as proteins were assumed be unstable under dry conditions. DBMs mimic human skin's structure offer both high...
We report the enhancement of structural stability a DNA nanotube (DNT) by changing salt concentrations for three different species, namely, NaCl, KCl, and MgCl2. Using fully atomistic molecular dynamics simulations, we find that, with gradual increment in NaCl concentration, DNT becomes compact rigid. The significant reduction average root-mean-square deviation, fluctuation, effective radius an increase concentration quantifies our observation. explain how DNT–ion interactions play vital...