A5068907724- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- dental development and anomalies
- Neonatal Respiratory Health Research
- Bone and Dental Protein Studies
- Reproductive System and Pregnancy
- Immune cells in cancer
- Endometriosis Research and Treatment
- Hedgehog Signaling Pathway Studies
- Dental Trauma and Treatments
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- TGF-β signaling in diseases
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
- Ovarian cancer diagnosis and treatment
- Caveolin-1 and cellular processes
- Pituitary Gland Disorders and Treatments
Hasselt University
2020-2025
KU Leuven
2020-2023
Biomedical Research Institute
2022
Ovarian cancer (OC) represents the most dismal gynecological cancer. Pathobiology is poorly understood, mainly due to lack of appropriate study models. Organoids, defined as self-developing three-dimensional in vitro reconstructions tissues, provide powerful tools model human diseases. Here, we established organoid cultures from patient-derived OC, particular prevalent high-grade serous OC (HGSOC). Testing multiple culture medium components identified neuregulin-1 (NRG1) key factor...
Abstract Ameloblasts are the specialized dental epithelial cell type responsible for enamel formation. Following completion of development in humans, ameloblasts lost and biological repair or regeneration is not possible. In past, vitro models to study epithelium ameloblast biology were limited freshly isolated primary cells immortalized lines, both with translational potential. recent years, large strides have been made induced pluripotent stem organoid this essential lineage – enabling...
Significance The pituitary is our master endocrine gland. Local damage and aging present important threats. We started to decrypt the ill-defined regulation of gland’s stem cells, typically dormant but acutely activated upon damage. Single-cell transcriptomics uncovered interleukin-6 as a cell activator local damage, corroborated in vivo vitro using cell–derived organoids. This competence extinguishes at aging, concurrent with raised inflammatory state older gland (inflammaging). However,...
Abstract Insight into human tooth epithelial stem cells and their biology is sparse. Tissue-derived organoid models typically replicate the tissue’s cell compartment. Here, we developed a first-in-time model starting from tooth. Dental follicle (DF) tissue, isolated unerupted wisdom teeth, efficiently generated organoids that were long-term expandable. The displayed stemness phenotype similar to DF’s rests of Malassez (ERM), compartment containing dental cells. Single-cell transcriptomics...
The pituitary represents the master gland governing endocrine system. We constructed a single-cell (sc) transcriptomic atlas of male mouse by incorporating existing and new data, spanning important postnatal ages in both healthy injured condition. demonstrate strong applicability this to unravel (patho)biology focusing on its stem cells investigating their complex identity (unveiling cell markers) niche (pinpointing regulatory factors). Importantly, we functionally validated findings using...
Cystic fibrosis (CF) is a life-shortening genetic disorder, caused by mutations in the CF transmembrane conductance regulator (CFTR) protein that regulates ion and fluid transport epithelial tissue. Female patients face considerable fertility challenges, with higher prevalence of deficient compared to healthy women. Not much known about underlying causes. In particular, pathobiology endometrium, uterus' inner lining essential for pregnancy expressing fluctuating CFTR levels during menstrual...
Organoid models provide powerful tools to study tissue biology and development in a dish. Presently, organoids have not yet been developed from mouse tooth. Here, we established tooth (TOs) early-postnatal molar incisor, which are long-term expandable, express dental epithelium stem cell (DESC) markers, recapitulate key properties of the tooth-type-specific manner. TOs display vitro differentiation capacity toward ameloblast-resembling cells, even more pronounced assembloids mesenchymal...
The pituitary represents the endocrine master regulator. In mouse, gland undergoes active maturation immediately after birth. Here, we in detail portrayed stem cell compartment of neonatal pituitary. Single-cell RNA-sequencing pictured an gland, revealing proliferative as well hormonal (progenitor) populations. pool displayed a hybrid epithelial/mesenchymal phenotype, characteristic development-involved tissue cells. Organoid culturing recapitulated cells’ interestingly also reproducing...
Single-cell (sc) omics has become a powerful tool to unravel tissue’s cell landscape across health and disease. In recent years, sc transcriptomic interrogation been applied variety of tooth tissues both human mouse, which considerably advanced our fundamental understanding biology. Now, an overarching integrated bird’s-view the mouse would be multi-faceted for dental research, enabling further decipherment biology development through constantly progressing state-of-the-art bioinformatic...
Abstract The pituitary represents the endocrine master regulator. In mouse, gland undergoes vivid maturation immediately after birth. Here, we in detail portrayed stem cell compartment of neonatal pituitary. Single-cell RNA-sequencing pictured an active gland, revealing proliferative as well hormonal (progenitor) populations. pool displayed a hybrid epithelial/mesenchymal phenotype, characteristic development-involved tissue cells. Organoid culturing recapitulated cells’ interestingly also...
Abstract The pituitary gland harbors a population of stem cells. However, role and regulation these cells remain poorly understood. We recently established organoids from mouse as novel research tool to explore cell biology (Cox et al., J. Endocrinol. 2019; 240:287-308). In general, represent 3D in vitro configurations that develop self-organize (single) tissue under well-defined culture conditions typically mirror the niche and/or embryogenic processes. Organoids reliably recapitulate key...