A5079785976- Inflammatory mediators and NSAID effects
- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Estrogen and related hormone effects
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Spectroscopy Techniques in Biomedical and Chemical Research
- Electrospun Nanofibers in Biomedical Applications
- Diamond and Carbon-based Materials Research
- Carbon Nanotubes in Composites
- Advanced Fluorescence Microscopy Techniques
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- Wound Healing and Treatments
- Tendon Structure and Treatment
- Force Microscopy Techniques and Applications
- Fullerene Chemistry and Applications
- Advanced Polymer Synthesis and Characterization
- TGF-β signaling in diseases
Hasselt University
2013-2017
Maastricht University Medical Centre
2016
Biomedical Research Institute
2016
Maastricht University
2011-2014
In the present study, we evaluated differentiation potential of human dental pulp stem cells (hDPSCs) toward Schwann cells, together with their functional capacity regard to myelination and support neurite outgrowth in vitro. Successful cell was confirmed at morphological ultrastructural level by transmission electron microscopy. Furthermore, compared undifferentiated hDPSCs, immunocytochemistry ELISA tests revealed increased glial marker expression neurotrophic factor secretion...
Despite the spontaneous regenerative capacity of peripheral nervous system, large gap nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow conduits in clinic urge need for alternative treatments. Recently, we have described promising neuroregenerative capacities Schwann cells derived from differentiated human dental pulp stem (d-hDPSCs) vitro. Here, extended vitro assays to show pro-angiogenic effects d-hDPSCs, such as...
Skeletogenesis and bone fracture healing involve endochondral ossification, a process during which cartilaginous primordia are gradually replaced by tissue. In line with role for cyclooxygenase-2 (COX-2) in the ossification process, non-steroidal anti-inflammatory drugs (NSAIDs) were reported to negatively affect due impaired osteogenesis. However, COX-2 activity chondrogenic phase of has not been addressed before. We show that fulfils an important regulatory function chondrocyte...
Objectives NSAIDs are used to relieve pain and decrease inflammation by inhibition of cyclooxygenase (COX)-catalyzed prostaglandin (PG) synthesis. PGs fatty acid mediators involved in cartilage homeostasis, however the action their synthesizing COX-enzymes differentiation is not well understood. In this study we hypothesized that COX-1 COX-2 have differential roles chondrogenic differentiation. Methods ATDC5 cells were differentiated presence (SC-560, Mofezolac) or (NS398, Celecoxib)...
Hypoxia promotes genetic instability and is therefore an important factor in carcinogenesis. We have previously shown that activation of the hypoxia responsive transcription HIFα can enhance mutagenic phenotype induced by environmental mutagen benzo[a]pyrene (BaP). To further elucidate mechanism behind ability to increase mutagenicity carcinogens, we examined detoxification BaP under hypoxic conditions. this end, human lung carcinoma cell line A549 was treated with 20%, 5% or 0.2% oxygen for...
In situ detection of MSCs remains difficult and warrants additional methods to aid with their characterization in vivo . Two‐photon confocal laser scanning microscopy (TPM) second harmonic generation (SHG) could fill this gap. Both techniques enable the cells extracellular structures, based on intrinsic properties specific tissue intracellular molecules under optical irradiation. TPM imaging SHG have been used for label‐free monitoring stem differentiation, assessment behavior biocompatible...
Abstract Hypoxia is characteristic for a number of pathologies and promotes genetic instability, as exposure to low oxygen levels results in an elevated mutation frequency. We have previously shown that activation the hypoxia responsive transcription factor HIF-1α, presence sufficient oxygen, enhanced frequencies induced by environmental mutagen benzo[a]pyrene (BaP). Since proper carcinogen metabolism depends on we examined how varying influenced BaP studying its deactivation. The human lung...