A5073571762- Microbial Inactivation Methods
- Mesenchymal stem cell research
- Toxin Mechanisms and Immunotoxins
- Wound Healing and Treatments
- Hematopoietic Stem Cell Transplantation
- Extracellular vesicles in disease
- Magnetic and Electromagnetic Effects
- MicroRNA in disease regulation
- Diamond and Carbon-based Materials Research
- Microfluidic and Bio-sensing Technologies
- Adenosine and Purinergic Signaling
- Transgenic Plants and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Sensor and Energy Harvesting Materials
- Carbon Nanotubes in Composites
- Conducting polymers and applications
- Periodontal Regeneration and Treatments
- Animal testing and alternatives
- Fullerene Chemistry and Applications
- 3D Printing in Biomedical Research
- Diabetic Foot Ulcer Assessment and Management
- Medical Imaging and Analysis
- Myofascial pain diagnosis and treatment
- Ultrasound and Cavitation Phenomena
- Cancer-related molecular mechanisms research
State Research Institute Centre for Innovative Medicine
2014-2025
Center for Physical Sciences and Technology
2015-2017
Vilnius University
2014
Biological systems are inherently complex and heterogeneous. Deciphering this complexity increasingly relies on high-throughput analytical methods tools that efficiently probe the cellular phenotype genotype. While recent advancements have enabled various single-cell -omics assays, their broader applications limited by challenge of conducting multi-step biochemical assays while retaining biological analytes. Extending our previous work ( 1 ) here we present a versatile technology based...
Calcium electroporation (CaEP) is an innovative approach to treating cancer, involving the internalization of supraphysiological amounts calcium through electroporation, which leads cell death. CaEP enables replacement chemotherapeutics (e.g., bleomycin). Here, we present a standard microsecond (μsCaEP) and novel high-frequency nanosecond protocols for (nsCaEP) elimination carcinoma tumors in C57BL/6J mice. We show efficacy eliminating increasing their survival rates vivo. The antitumor...
Gene delivery by the pulsed electric field is a promising alternative technology for nonviral transfection; however, application of short pulses (i.e., nanosecond) extremely limited. In this work, we aimed to show capability improve gene using MHz frequency bursts nanosecond and characterize potential use gold nanoparticles (AuNPs: 9, 13, 14, 22 nm) in context. We have used 3/5/7 kV/cm × 300 ns 100 compared efficacy parametric protocols conventional microsecond (100 µs 8, 1 Hz) separately...
Extracellular vesicles (EVs) effectively suppress neuroinflammation and induce neuroprotective effects in different disease models. However, the mechanisms by which EVs regulate neuroinflammatory response of microglia remains largely unexplored. Here, we addressed this issue testing action derived from human exfoliated deciduous teeth stem cells (SHEDs) on immortalized microglial cells. We found that induced a rapid increase intracellular Ca2+ promoted significant ATP release after 20 min...
In this work, a time-dependent and time-independent study on bleomycin-based high-frequency nsECT (3.5 kV/cm × 200 pulses) for the elimination of LLC1 tumours in C57BL/6J mice is performed. We show efficiency (200 ns 700 delivered at 1 kHz MHz) increase their survival. The dynamics immunomodulatory effects were observed after electrochemotherapy by investigating immune cell populations antitumour antibodies different timepoints treatment. ECT treatment resulted an increased percentage CD4+...
Mesenchymal stromal cells (MSCs, known as mesenchymal stem cells) are considered to be a promising therapeutic tool for many diseases. But it is still unclear which more efficient and safe wound healing tissue regeneration clinical applications: undifferentiated, partially differentiated or cells. In this study, we modified MSCs with keratinocyte-conditioned medium (KCM) examined MSCs, (PMSCs) cell migration, accumulation in the wounded area well regenerative efficiency full-thickness skin...
High power pulsed generator for non-contact cell electroporation application in magnetic fields is presented. Finite element method analysis of the field and Jurkat T lymphoblasts experimental data are overviewed. The dependence efficiency on structure microcoil was acquired experimentally. Possible problems technique, further developments result overview discussed.
Cell-based therapy is a promising strategy for promoting tissue regeneration when conventional treatments are not effective. ehT choice of the accessible source to obtain sufficient cell amount and use suitable biomaterials improve delivery efficiency main tasks safe, effective, reliable application stem therapy. In this study, we have compared influence bone marrow-derived Lin¯ cells on skin after local transplantation with or without type I collagen-based gel in BALB/c mice full-thickness...
Abstract Due to their unique properties, bone marrow-derived Lin− cells can be used regenerate damaged tissues, including skin. The objective of our study was determine the influence skin tissue-specific microenvironment on mouse cell proliferation and migration in vitro. Cells were analyzed for expression stem/progenitor surface markers by flow cytometry. Proliferation MACS-purified 3D cultures investigated WST-8 assay. evaluated vitro scratch results obtained show that basement membrane...
Extracellular vesicles (EVs) effectively suppress neuroinflammation and induce neuroprotective effects in different disease models. However, the mechanisms by which EVs regulate neuroinflammatory response of microglia remain largely unexplored. Here, we addressed this issue testing action derived from human exfoliated deciduous teeth stem cells (SHEDs) on immortalized microglial cells. We found that induced a rapid increase intracellular Ca2+ promoted significant ATP release after 20 min...
Abstract The objective of this work was to examine the migration transplanted bone marrow hematopoietic lin− cell population using BALB/c mouse contact hypersensitivity model in vivo and determine time point at which they reach site injury (paw edema) as well other undamaged organs, such liver spleen. Female mice with induced reaction were intravenously injected 1×106 cells/mouse cells, labeled PKH67. presence stained cells tissue sections evaluated by fluorescent microscopy. After one hour,...