A5090581148- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Toxin Mechanisms and Immunotoxins
- Magnetic and Electromagnetic Effects
- Transgenic Plants and Applications
- Viral gastroenteritis research and epidemiology
- Animal health and immunology
- Viral Infectious Diseases and Gene Expression in Insects
- Infant Nutrition and Health
- Ultrasound and Cavitation Phenomena
- Planarian Biology and Electrostimulation
- 3D Printing in Biomedical Research
- Food Drying and Modeling
- bioluminescence and chemiluminescence research
- Reproductive Physiology in Livestock
- Plasma Applications and Diagnostics
State Research Institute Centre for Innovative Medicine
2021-2024
Vilnius Gediminas Technical University
2022-2024
National Institute of Standards and Technology
2023
Université de Lorraine
2023
this work focuses on bleomycin electrochemotherapy using new modality of high repetition frequency unipolar nanosecond pulses.As a tumor model, Lewis lung carcinoma (LLC1) cell line in C57BL mice (n = 42) was used. Electrochemotherapy performed with intertumoral injection (50 μL 1500 IU solution) followed by and microsecond range electrical pulse delivery via parallel plate electrodes. The 3.5 kV/cm pulses 200 700 ns were delivered burst at frequencies 1 kHz MHz. For comparison treatment...
Calcium electroporation (CaEP) is an innovative approach to treating cancer, involving the internalization of supraphysiological amounts calcium through electroporation, which leads cell death. CaEP enables replacement chemotherapeutics (e.g., bleomycin). Here, we present a standard microsecond (μsCaEP) and novel high-frequency nanosecond protocols for (nsCaEP) elimination carcinoma tumors in C57BL/6J mice. We show efficacy eliminating increasing their survival rates vivo. The antitumor...
Gene transfer into primary immune cells as well cell lines is essential for scientific and therapeutical applications. One of the methods used gene electroporation (EP). EP a method where pulsed electric field (PEF) causes highly transient permeability targeted membrane. In this work, we present electrotransfection CHO-K1, 4T1 lines, murine DCs with detectable protein-encoding plasmids in sub-microsecond range. Microsecond (µs)- nanosecond (ns)-range transfection protocols were used. The...
Bovine colostrum (BC) is the first milk produced by lactating cows after parturition. BC rich in various amino acids, proteins, and fats essential for nutrition of neonate calves. Despite evident beneficial effect on calves, blood biomarkers poorly understood. Calves that received showed significantly higher body mass at days 7 30 (38.54 kg 43.42 kg, respectively) compared to replacer group (p = 0.0064). induced greater quantities neutrophils (0.27 × 109/L) monocytes (4.76 comparison (0.08...
Gene delivery by the pulsed electric field is a promising alternative technology for nonviral transfection; however, application of short pulses (i.e., nanosecond) extremely limited. In this work, we aimed to show capability improve gene using MHz frequency bursts nanosecond and characterize potential use gold nanoparticles (AuNPs: 9, 13, 14, 22 nm) in context. We have used 3/5/7 kV/cm × 300 ns 100 compared efficacy parametric protocols conventional microsecond (100 µs 8, 1 Hz) separately...
Invasive infections caused by drug-resistant bacteria are a problem responsible for many fatal cases, especially in burn wound care centers, while bacterial resistance to antibiotics is growing dramatically worldwide. In this work, we utilize pulsed electric fields (up 25 kV/cm × 750 ns) combination with low-concentration (1%) acetic acid the inactivation of P. aeruginosa. An vivo superficial infection model developed BALB/C mice using luminescent strain We show that an up field (3 kV, 1.2...
Bovine colostrum (COL), the first milk secreted by lactating cows postpartum, is a rich source of bioactive compounds that exert significant role in survival, growth, and immune development neonatal calves. This study investigated immunomodulatory effects COL on cytokine production vitro using Caco-2/THP-1 macrophage co-culture model stimulated with Phorbol 12-myristate 13-acetate (PMA). pretreatment significantly reduced IL-6 (241.3 pg/mL) induced PMA (p < 0.05), while increasing IL-10...
The phenomenon known as bipolar cancellation is observed when biphasic nanosecond electric field pulses are used, which results in reduced electroporation efficiency compared to unipolar of the same parameters. Basically, negative phase pulse diminishes effect positive phase. Our study aimed investigate how affects Ca2+ electrochemotherapy and cellular response under varying intensities durations (3-7 kV/cm, 100, 300, 500 ns 1 MHz repetition frequency bursts, n = 100). As a reference,...
Electrochemotherapy (ECT) is a safe and efficient method of targeted drug delivery using pulsed electric fields (PEF), one that based on the phenomenon electroporation. However, problems field homogeneity within tumor can cause diminishing treatment efficacy, resulting only in partial response to procedure. This work used gold nano-particles for amplification, introducing capability improve available elec-trochemotherapy methods solve associated with non-homogeneity.
In this work, a time-dependent and time-independent study on bleomycin-based high-frequency nsECT (3.5 kV/cm × 200 pulses) for the elimination of LLC1 tumours in C57BL/6J mice is performed. We show efficiency (200 ns 700 delivered at 1 kHz MHz) increase their survival. The dynamics immunomodulatory effects were observed after electrochemotherapy by investigating immune cell populations antitumour antibodies different timepoints treatment. ECT treatment resulted an increased percentage CD4+...
Abstract Bovine colostrum (COL), the first milk secreted by lactating cows postpartum, is a rich source of bioactive compounds that exert significant role on survival, growth, and immune development neonatal calves [9,10]. This study investigated immunomodulatory effects COL cytokine production in vitro using Caco-2/THP-1 macrophage co-culture model stimulated with Phorbol 12-myristate 13-acetate (PMA). pretreatment significantly reduced IL-6 induced PMA, while increasing IL-10 production....
Short electric field pulses represent a novel potential approach for achieving uniform electroporation within tissue containing elongated cells oriented in various directions, such as electroporation-based cardiac ablation procedures. In this study, we investigated how with nanosecond respect to different pulse shapes (unipolar, bipolar, and asymmetric) influences cardiomyocyte permeabilization gene transfer. For purpose, rat cardiomyocytes (H9c2) were used. The efficacy of the pulsed...
Gene electrotransfer (GET) is non-viral gene delivery technique, also known as electroporation-mediated or electrotransfection. GET a method used to introduce foreign genetic material (such DNA RNA) into cells by applying external pulsed electric fields (PEFs) create temporary pores in the cell membrane. This study was undertaken examine impact of buffer composition on efficiency mammalian Also, we specifically compared effectiveness high-frequency nanosecond (ns) pulses with standard...
Electroporation-based procedures employing nanosecond bipolar pulses are commonly linked to an undesirable phenomenon known as the cancelation effect. The cancellation effect arises when second pulse partially or completely neutralizes effects of first pulse, simultaneously diminishing cells’ plasma membrane permeabilization and overall efficiency procedure. Introducing a temporal gap between positive negative phases during electroporation may help overcome phenomenon; however, exact...
Long duration electric pulses are frequently used to facilitate DNA electrotransfer into cells and tissues, while electroporation can be combined with electrophoresis maximize the transfection efficiency. In this work, we present dielectrophoresis (DEP)-assisted methodology for of plasmid (3.5 kbp pmaxGFP) mammalian (CHO-K1). A prototype an cuvette center needle electrode DEP-assisted is presented resulting in a 1.4-fold efficiency increase compared electroporation-only procedure (1.4 kV/cm...