A5090406358- Extracellular vesicles in disease
- Mesenchymal stem cell research
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Cancer Cells and Metastasis
- Bone Tissue Engineering Materials
- Adenosine and Purinergic Signaling
- Periodontal Regeneration and Treatments
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Marine Biology and Environmental Chemistry
- Pluripotent Stem Cells Research
- Cell death mechanisms and regulation
- Oral microbiology and periodontitis research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Connexins and lens biology
- Immune Response and Inflammation
- Orthopaedic implants and arthroplasty
- Circular RNAs in diseases
State Research Institute Centre for Innovative Medicine
2010-2023
Vilnius University
2007-2010
Abstract Parkinson's disease (PD) is the second most common neurodegenerative disorder affecting millions of people worldwide. At present, there no effective cure for PD; treatments are symptomatic and do not halt progression neurodegeneration. Extracellular vesicles (EVs) can cross blood–brain barrier represent promising alternative to classical treatment strategies. In present study, we examined therapeutic effects intranasal administration EVs derived from human exfoliated deciduous teeth...
Human dental pulp derived from exfoliated deciduous teeth has been described as a promising alternative source of multipotent stem cells. While these cells share certain similarities with mesenchymal stem-like (MSC) isolated other tissues, basically they are still poorly characterized. In this study, for the first time, proteomic map abundantly expressed proteins in stromal human (SHED) was established. We also analyzed signatures 2 clonal strains SHEDs by single-cell cloning. The were...
Periodontitis is an infectious disease characterized by chronic inflammation and progressive destruction of periodontal tissues. Chronic inflammatory environment may affect immunomodulatory function ligament stem cells (PDLSCs) promote shift toward proinflammatory phenotype contributing to propagation periodontitis. Therefore, suppression response in PDLSCs represents a novel therapeutic approach. Extracellular vesicles (EVs) have been shown display anti-inflammatory immunosuppressive...
Functional impairments of microglia have been recently associated with several neurological conditions. Therefore, modulation anti-inflammatory and phagocytic properties microglial cells could represent a novel therapeutic approach. In the present study, we investigated effects extracellular vesicles (EVs) derived from stem dental pulp human exfoliated deciduous teeth (SHEDs) on inflammatory response functional immortalized cells. NFκB reporter assays demonstrated that EVs suppressed...
Extracellular vesicles (EVs) effectively suppress neuroinflammation and induce neuroprotective effects in different disease models. However, the mechanisms by which EVs regulate neuroinflammatory response of microglia remains largely unexplored. Here, we addressed this issue testing action derived from human exfoliated deciduous teeth stem cells (SHEDs) on immortalized microglial cells. We found that induced a rapid increase intracellular Ca2+ promoted significant ATP release after 20 min...
Abstract Targeting of disease‐associated microglia represents a promising therapeutic approach that can be used for the prevention or slowing down neurodegeneration. In this regard, use extracellular vesicles (EVs) approach. However, molecular mechanisms by which EVs regulate microglial responses remain poorly understood. present study, we derived from human oral mucosa stem cells (OMSCs) to investigate effects on lipid raft formation and phagocytic response cells. Lipid labeling with...
Current cell therapy protocols require considerable numbers of mesenchymal stromal cells (MSCs), which can be obtained only by in vitro expansion. The most important issue is a choice optimal growth supplements for culture. Ideally, these should known composition, free animal components and allow production large homogenic populations MSCs considerably short period time. Since this standard has not been achieved to date, we aimed assess the molecular responses different commonly use. were...
Intrinsic tissue regeneration mechanisms are still not fully understood. The destruction/reconstruction processes usually in fine balance; however, this can be easily destroyed, for example the environment of chronic inflammation. One major proteins present at inflammatory sites is multifunctional protein alpha-1-antitrypsin (AAT). In study, potential therapeutic effects human antiprotease on progenitor cells assessed.Stromal from exfoliated deciduous teeth (SHEDs) were used, which similar...
Extracellular vesicles (EVs) effectively suppress neuroinflammation and induce neuroprotective effects in different disease models. However, the mechanisms by which EVs regulate neuroinflammatory response of microglia remain largely unexplored. Here, we addressed this issue testing action derived from human exfoliated deciduous teeth stem cells (SHEDs) on immortalized microglial cells. We found that induced a rapid increase intracellular Ca2+ promoted significant ATP release after 20 min...
Abstract Impairments of the blood brain barrier (BBB) and vascular dysfunction contribute to Alzheimer’s disease (AD) from earliest stages. However, effects AD-affected astrocytes on BBB remain largely unexplored. In present study we created an in vitro using human immortalised endothelial cells combination with astroglial cell lines hippocampus 3xTG-AD wild-type mice (3Tg-iAstro WT-iAstro, respectively). We found that co-culturing monolayers WT-iAstro up-regulates expression tight junction...