A5079773896- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Alkaline Phosphatase Research Studies
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- Cellular transport and secretion
- Antifungal resistance and susceptibility
- Peptidase Inhibition and Analysis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Vitamin D Research Studies
- Protease and Inhibitor Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- Biochemical and Molecular Research
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Streptococcal Infections and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Epilepsy research and treatment
- Pharmacogenetics and Drug Metabolism
- Analytical Methods in Pharmaceuticals
Kumamoto University Hospital
2016-2025
Kumamoto City Hospital
2024-2025
Kumamoto University
2022
City Hospital
2013
Omuta hospital
2011-2013
Niigata University
1993-2010
Kagoshima University
1999
Kyoto Institute of Technology
1997
Massachusetts Institute of Technology
1996
Fukuoka University
1986-1992
The antiviral antibiotic brefeldin A (BFA) strongly inhibits the protein secretion in cultured rat hepatocytes (Misumi, Y., Misumi, Miki, K., Takatsuki, A., Tamura, G., and Ikehara, Y. (1986) J. Biol. Chem. 261, 11398-11403). We have further examined inhibitory effect of drug on intracellular transport albumin by an immunocytochemical technique with peroxidase-conjugated Fab fragments anti-rat IgG. In treated BFA (2.5 micrograms/ml) for 1 h at 37 degrees C, no characteristic structures Golgi...
The present study indicates that crystalline elastase of Pseudomonas aeruginosa is a very potent inactivator human plasma alpha 1-proteinase inhibitor, the enzyme (E) inactivated inhibitor (I) almost completely within 1 h at 25 degrees C molar ratio E/I = 1:100. P. protease also but 100-fold less. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated by and showed decreases in molecular weight approximately 5,000 10,000, respectively. Regeneration trypsin was negligible even...
We have cloned a rat cDNA encoding furin which is structurally related to yeast Kex2 protease. Products of 88 and 94 kDa were obtained by in vitro transcription/translation the absence presence microsomes. When was transfected into COS-1 cells, expressed as major glycosylated form kDa, accompanied minor proteolytic 86 found be localized Golgi complex. Proalbumin complement pro-C3 are intracellularly processed their mature forms cleavage at dibasic residues Arg-Arg, common signal many...
A soluble form of tissue-nonspecific alkaline phosphatase was purified to apparent homogeneity from the culture media Sf9 cells which had been infected with recombinant baculoviruses encoding human (TNSALP). To facilitate purification, an oligonucleotide consisting 6 tandem codons for histidine and a stop codon engineered into TNSALP cDNA. The molecular mass enzyme through nickel-chelate column estimated be 54 kDa by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. That...
Area under the concentration-time curve (AUC)-guided vancomycin treatment is associated with decreased nephrotoxicity. It preferable to obtain two samples estimate AUC. This study examined usefulness of AUC estimation via trough concentration (Cmin)-only sampling 260 adults infected methicillin-resistant Staphylococcus aureus (MRSA) who received vancomycin. The exact Cmin time was used for Bayesian estimation. A significantly higher early response observed in patients a day 2 ≥ 400 µg·h/mL...
Abstract Background Cataract surgeries are increasing annually, making appropriate medical management essential. The routine use of systemic antimicrobial agents for preventing surgical site infections lacks strong evidence and may increase the risk drug-resistant bacteria adverse events. This study examined impact discontinuing cefazolin (CEZ) administration during perioperative period cataract surgery on incidence postoperative events costs. Methods Inpatient patients were divided into two...
Abstract Aim Area under the concentration‐time curve (AUC)‐guided dosing is recommended in vancomycin therapy. This study aims to determine empirical for achieving an AUC on first day (AUC f24h ) and optimal number of samplings Bayesian estimation. Methods A multicentric prospective was conducted across five hospitals. Critically ill patients were included, with rich sampling and/or days 3‐5 Cumulative dose trapezoidal > 400 μg·h/mL predictive performances clearance limited evaluated. The...
The biosynthesis and post-translational modification of placental alkaline phosphatase were studied in human choriocarcinoma cells, JEG-3. Pulse-chase experiments with [35S]methionine demonstrated that was synthesized as a major precursor form Mr 63,000, which then converted to mature 66,000, by processing its N-linked oligosaccharides from the high-mannose type complex type. In addition, two forms protein found be modified glycophospholipid, components characterized metabolic incorporation...
In this study, we aimed to evaluate limited sampling strategies for achieving the therapeutic ranges of area under concentration-time curve (AUC) vancomycin on first and second day (AUC
We examined the effects of weakly basic amines on secretion and post-translational modifications secretory proteins in cultured rat hepatocytes. Weakly such as methylamine, chloroquine NH4Cl strongly inhibited not only protein secretion, but also proteolytic conversion a proform complement C3, allowing precursor to be released into medium. The amines, however, had no effect prohaptoglobin its subunits. Since available evidence indicates that pro-C3 occurs at Golgi complex while takes place...
The effects of calcium depletion on the proteolytic cleavage and secretion plasma protein precursors were investigated in primary cultured rat hepatocytes HepG2 cells. When cells incubated with A23187, calcium-specific ionophore, a medium lacking CaCl2, serum albumin third fourth components complement, C3 C4, respectively, found to be released into medium. addition ionomycin or EGTA inhibited processing pro-C3 as well. Blocking secretory pathway either at mixed endoplasmic reticulum/Golgi...
This study aimed to elucidate the ultrastructural role of Gla proteins in bone mineralization by means a warfarin-administration model. Thirty-six 4-week-old male F344 rats received warfarin (warfarin group) or distilled water (control group), and were fixed after 4, 8 12 weeks with an aldehyde solution. Tibiae femora employed for histochemical analyses alkaline phosphatase, osteocalcin tartrate-resistant acid histomorphometry electron microscopy. After weeks, there no marked...
On the basis of characterizations a set UmuD monocysteine derivatives, we had suggested that positions 24, 34, and 44 are closer to intact homodimer interface than other tested (M. H. Lee, T. Ohta, G. C. Walker, J. Bacteriol. 176:4825-4837, 1994). Because this region also appeared be important for interactions with RecA, followed up on our previous study by constructing second derivatives single cysteine substitutions at 30 42. We found like VC34 mutant, 32 35 showed deficiencies in vivo...
Tissue-non-specific alkaline phosphatase (TNSALP) with an Arg(54)-->Cys (R54C) or Asp(277)-->Ala (D277A)substitution was found in a patient hypophosphatasia [Henthorn,Raducha, Fedde, Lafferty and Whyte (1992) Proc. Natl. Acad. Sci. U.S.A.89, 9924-9928]. To examine effects of these missense mutations onproperties TNSALP, the TNSALP mutants were expressed ectopically inCOS-1 cells. The wild-type synthesized as 66-kDa endo-beta-N-acetylglucosaminidase H (Endo H)-sensitive form, processed to...
We have studied the effect of brefeldin A (BFA) on intracellular transport envelope proteins vesicular stomatitis virus (VSV) and sindbis in primary cultured rat hepatocytes. BFA (2.5 micrograms/ml) inhibited not only secretion plasma into medium, but also assembly both G protein VSV E1 E2 (envelope proteins) respective virions. Concomitantly, acquisition endo-beta-N-acetylglucosaminidase H resistance by proteolytic conversion PE2 to were found be BFA-treated cells, suggesting that was...
The signaling axis comprising the parathyroid hormone (PTH)-related peptide (PTHrP), PTH/PTHrP receptor and fibroblast growth factor 3 (FGFR3) plays a central role in chondrocyte proliferation. Indian hedgehog (IHH) gene is normally expressed early hypertrophic chondrocytes, its negative feedback loop was shown to regulate signaling. In this study, we examined regulation of expression FGFR3-transfected chondrocytic cell line, CFK2. Expression IHH could not be verified on these cells, with...