A5073155517- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Oral microbiology and periodontitis research
- Proteoglycans and glycosaminoglycans research
- Fibroblast Growth Factor Research
- dental development and anomalies
- Extracellular vesicles in disease
- Connective tissue disorders research
- Dental Radiography and Imaging
- Nuclear Physics and Applications
- Bone and Dental Protein Studies
- HIV/AIDS oral health manifestations
- Oral Health Pathology and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Hypoxia, and Metabolism
- Advanced NMR Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Muon and positron interactions and applications
- Dental Health and Care Utilization
- Energy and Environment Impacts
- Oral and gingival health research
- Dermatologic Treatments and Research
- melanin and skin pigmentation
- Erythrocyte Function and Pathophysiology
- Nanoplatforms for cancer theranostics
Osaka University
2010-2025
Oklahoma Medical Research Foundation
2013-2015
Fibrosis is a common disease process in which profibrotic cells disturb organ function by secreting disorganized extracellular matrix (ECM). Adipose tissue fibrosis occurs during obesity and associated with metabolic dysfunction, but how originate still being elucidated. Here, we use developmental model to investigate perivascular white adipose (WAT) their potential cause fibrosis. We show that Nestin-Cre transgene targets (adventitial pericyte-like cells) WAT, Nestin-GFP specifically labels...
Mineralization is the most fundamental process in vertebrates. It predominantly mediated by osteoblasts, which secrete mineral precursors, likely through matrix vesicles (MVs). These vesicular structures are calcium and phosphate rich contain organic material such as acidic proteins. However, it remains largely unknown how intracellular MVs transported secreted. Here, we use scanning electron-assisted dielectric microscopy super-resolution for assessing live osteoblasts mineralizing...
Abstract Fibroblast growth factor‐2 (FGF‐2) regulates a variety of functions the periodontal ligament (PDL) cell, which is key player during tissue regeneration following breakdown by disease. In this study, we investigated effects FGF‐2 on cell migration and related signaling pathways MPDL22, mouse PDL clone. activated MPDL22 cells phosphorylation phosphatidylinositol 3‐kinase (PI3K) akt. The P13K inhibitors, Wortmannin LY294002, suppressed both akt activation in suggesting that PI3K/akt...
Although annexin A1 (ANXA1) is known to mediate inflammatory responses through N-formyl peptide receptor 2 (FPR2), the role of ANXA1-FPR2 signaling pathway in periodontal disease remains unclear. This study investigated contribution this pathophysiology disease. Using a ligature-induced mouse model, we performed histologic analyses examine ANXA1 and FPR2 expression. We observed upregulation within gingiva ligament. In vitro analysis human ligament cells revealed that interleukin 1β...
Adapter trimming and removal of duplicate reads are common practices in next-generation sequencing pipelines. Sequencing ambiguously mapped to repetitive low complexity regions can also be problematic for accurate assessment the biological signal, yet their impact on data has not received much attention. We investigate how adapters, removing duplicates, filtering out overlapping influence significance signal RNA- ChIP-seq experiments.We assessed effect processing steps alignment statistics...
The new 2018 classification of periodontal diseases is reported to be related tooth loss due disease (TLPD) during supportive therapy (SPT). However, few reports have evaluated this relationship for Asians or analyzed the association and TLPD by distinguishing between active (APT) SPT. In study, we retrospectively applied 607 Japanese periodontitis patients examined annual rates per patient respective periods. were higher in stage IV and/or grade C both APT SPT was not associated with...
ABSTRACT Periodontal tissue supports teeth in the alveolar bone socket via fibrous attachment of periodontal ligament (PDL). The PDL contains fibroblasts and stem/progenitor cells, collectively known as cells (PDLCs), on top osteoblasts cementoblasts surface cementum, respectively. However, characteristics lineage hierarchy each cell type remain poorly defined. This study identified associated protein-1 (Plap-1) a PDL-specific extracellular matrix protein. We generated knock-in mice...
Adipose tissue is an attractive source for somatic stem cell therapy. Currently, human adipose tissue-derived stromal cells/mesenchymal cells (hADSCs/MSCs) are cultured with fetal bovine serum (FBS). Recently, however, not only embryonic lines on mouse feeder but also bone marrow-derived MSCs FBS were reported to express N-glycolylneuraminic acid (Neu5Gc) xenoantigen. Human contains high titers of natural preformed antibodies against Neu5Gc. We studied the presence Neu5Gc hADSCs/MSCs and...
Abstract Background Cellular responses to hypoxia regulate various biological events, including angiogenesis and extracellular matrix metabolism. Collagen is a major component of the in periodontal tissues its coordinated production essential for tissue homeostasis. In this study, we investigated effects on collagen human gingival fibroblasts (HGFs) ligament cells (HPDLs). Methods HGFs HPDLs were cultured under either normoxic (20% O 2 ) or hypoxic (1% conditions. Nuclear expression...
Iwayama T, Yanagita M, Mori K, Sawada Ozasa Kubota Miki Kojima Y, Takedachi Kitamura Shimabukuro Hashikawa Murakami S. Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells. J Periodont Res 2012; 47: 563–571. © 2012 John Wiley & Sons A/S Background Objective: is a cytokine constitutively produced by adipocytes exhibits multiple biological targeting various cell types. However, the effects adiponectin on primary cells are still unexplored. Therefore, we...
Abstract Periodontal disease is a chronic inflammatory condition that affects various peripheral organs. The periodontal inflamed surface area (PISA) quantifies periodontitis severity and the spread of wounds. This study aimed to investigate association between PISA high-sensitivity C-reactive protein (hs-CRP), systemic inflammation marker. included 250 community-dwelling septuagenarians (69–71 years). We collected information on their medical (e.g., diabetes dyslipidemia) dental...
Abstract Adipose tissue fibrosis with chronic inflammation is a hallmark of obesity-related metabolic disorders, and the role proteoglycans in developing adipose interest. Periodontal disease associated obesity; however, underlying molecular mechanisms remain unclear. Here we investigated roles periodontal ligament protein-1 (PLAP-1)/asporin, proteoglycan preferentially highly expressed ligament, dysfunction adipocyte differentiation. It was found that PLAP-1 also white tissues. Plap-1...
Periodontal ligament (PDL) possesses a stem/progenitor population to maintain the homeostasis of periodontal tissue. However, transcription factors that regulate this have not yet been identified. Thus, we aimed identify molecule related osteogenic differentiation PDL progenitors using single cell-based strategy in study. We first devised new protocol isolate cells from surface adult murine molars and established 35 cell-derived clones explant. Among these clones, six with high (high n = 3)...
Periodontitis is a chronic inflammatory disease that destroys tooth-supporting periodontal tissue. Current regenerative therapies have unsatisfactory efficacy; therefore, tissue engineering might be established by developing new cell-based therapies. In this study, we evaluated the safety and efficacy of adipose tissue-derived multi-lineage progenitor cells (ADMPC) autologous transplantation for regeneration in humans. We conducted an open-label, single-arm exploratory phase I clinical study...
Periodontal ligament-associated protein 1 (PLAP-1), also known as Asporin, is an extracellular matrix expressed in the periodontal ligament and plays a crucial role tissue homeostasis. Our previous research demonstrated that PLAP-1 may inhibit TLR2/4-mediated inflammatory responses, thereby exerting protective function against periodontitis. However, precise roles of (PDL) its relationship to periodontitis have not been fully explored. In this study, we employed knockout mice investigate...
Abstract PM2.5 has been correlated with risk factors for various diseases and infections. It promotes tissue injury by direct effects of particle components. However, on cells have not fully investigated. Recently, we developed a novel imaging technology, scanning electron-assisted dielectric-impedance microscopy (SE-ADM), which enables observation biological specimens in aqueous solution. In this study, successfully observed incorporated into living mammalian culture media. Our system...
To investigate the mutual regulation of hypoxia-inducible factor (HIF)-1α activity and periodontal ligament-associated protein-1 (PLAP-1) expression in human ligament cells (HPDLs).Cellular responses to hypoxia regulate various biological events (e.g., inflammation tissue regeneration) through activation HIF-1α. PLAP-1, an extracellular matrix protein preferentially expressed ligament, plays important roles functions HPDLs. Although PLAP-1 has been demonstrated hypoxic regions, involvement...
Highlights•Direct observation of melanosomes in living cells by SE-ADM.•Melanosomes MNT-1 have a toroidal structure.•Shape differs depending on cell type.•DNN enables highly accurate detection SE-ADM images.AbstractMelanins are the main pigments found mammals. Their synthesis and transfer to keratinocytes been widely investigated for many years. However, analysis has mainly carried out using fixed rather than live cells. In this study, we analysed mammalian newly developed scanning...