A5105976789- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Bone Metabolism and Diseases
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- Cell Adhesion Molecules Research
- Macrophage Migration Inhibitory Factor
- Clostridium difficile and Clostridium perfringens research
- NF-κB Signaling Pathways
- Angiogenesis and VEGF in Cancer
- Platelet Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Chemokine receptors and signaling
- Venous Thromboembolism Diagnosis and Management
- Food Allergy and Anaphylaxis Research
- Mast cells and histamine
- Antimicrobial Peptides and Activities
- Neurogenetic and Muscular Disorders Research
- Neurogenesis and neuroplasticity mechanisms
- Asthma and respiratory diseases
- Kawasaki Disease and Coronary Complications
- Innovation and Socioeconomic Development
Aichi Medical University
2019-2024
Tohoku University
2007-2021
Wakayama Medical University
2008-2011
Japan Science and Technology Agency
2008
Okayama University
1997-1998
Kyushu University
1974
BM-derived endothelial progenitor cells (EPCs) are critical and essential for neovascularization in tissue repair tumorigenesis. EPCs migrate from BM to tissues via the bloodstream, but specific chemotactic cues have not been identified. Here we show mice that absence of CCR5 reduced vascular EPC accumulation neovascularization, macrophage recruitment, eventually delayed healing wounded skin. When transferred into Ccr5-/- mice, Ccr5+/+ cells, accumulated wound site, were incorporated...
We have isolated a cDNA clone encoding novel murine cell-surface glycoprotein. This polypeptide is predicted to be composed of signal peptide 23 amino acids, an extracellular region 620 acids that contains six immunoglobulin-like domains with five potential N-glycosylation sites, transmembrane sequence 20 and cytoplasmic tail 178 four sets sequences similar the immunoreceptor tyrosine-based inhibition motif. The relative molecular mass mature calculated 90,520 Da. polypeptide, designated as...
Abstract Osteoclasts, multinucleated cells of myeloid-monocytic origin, are responsible for bone resorption, which is crucial maintenance homeostasis in concert with bone-forming osteoblasts nonhematopoietic, mesenchymal origin. Receptor activator NF-κB ligand (RANKL) and M-CSF, expressed on the surface secreted by osteoblasts, respectively, essential factors that facilitate osteoclast formation. In contrast to activation processes formation, inhibitory mechanisms it poorly understood....
Deep vein thrombosis (DVT) is a major cause of pulmonary thromboembolism, leading death in individuals with DVT. Several lines evidence indicate proinflammatory cytokines such as TNF-α are involved thrombus formation and resolution, but the roles IFN-γ remain unclear. To address this issue, we performed ligation inferior vena cava to induce DVT WT IFN-γ-deficient (Ifng-/-) mice. In mice, intrathrombotic levels were elevated progressively postligation interval was extended. Thrombus size...
Osteoclasts, cells of myeloid lineage, play a unique role in bone resorption, maintaining skeletal homeostasis concert with bone-producing osteoblasts. Osteoclast development and maturation (osteoclastogenesis) is driven by receptor activator NF-κB ligand macrophage-colony stimulating factor invariably requires signal initiated immunoreceptor tyrosine-based activation motif (ITAM)-harboring Fc common γ chain or DNAX-activating protein (DAP)12 (also referred to as KARAP TYROBP) that...
We have analyzed the molecules participating in inhibitory function of gp49B1, a murine type I transmembrane glycoprotein expressed on mast cells and natural killer cells, as well chromosomal location its gene. As assessed by SDS-polyacrylamide gel electrophoresis immunoblot analysis, tyrosine-phosphorylated, but not nonphosphorylated, synthetic peptides matching each two immunoreceptor tyrosine-based motif (ITIM)-like sequences found cytoplasmic portion gp49B1 associated with ∼65-kDa...
Abstract CD20+CD27+CD43+ B (CD43+ B) cells have been newly defined among PBMCs and proposed to be human B1 cells. However, it is controversial as whether they are orthologs of murine how related other B-cell populations, particularly CD20+CD27+CD43− memory CD20lowCD27highCD43high plasmablasts. Our objective identify phenotypically the position CD43+ peripheral B-lineage cell compartments in healthy donors, with reference subsets from patients systemic lupus erythematosus (SLE). We found that...
Plasmablasts and plasma cells (PBs PCs) producing pathogenic auto-antibodies in patients with systemic autoimmune diseases could be a better target for specific therapies the disease than general immunosuppression or pan- activated B-cell targeting. Our previous study indicated that leukocyte immunoglobulin-like receptor (LILR) B4 (B4, also known as ILT3/LIR-5/CD85k), tolerogenic antigen-presenting cells, is ectopically expressed on PB/PC surface healthy individuals. Here, we show enlarged...
Abstract The extracellular matrix (ECM) is the basis for virtually all cellular processes and also related to tumor metastasis. Fibronectin (FN), a major ECM macromolecule expressed by different cell types present in plasma, consists of multiple functional modules that bind ECM-associated, cell-surface proteins such as integrins FN itself, thus ensuring its cell-adhesive modulatory role. Here we show constitutes an immune checkpoint. Thus, was identified physiological ligand...
The injection of Clostridium difficile toxin A into the ileal loops caused fluid accumulation with destruction intestinal epithelial structure and recruitment neutrophils macrophages. Concomitantly, intraileal gene expression CX3CL1/fractalkine (FKN) its receptor, CX3CR1, was enhanced. When treated in a similar manner, CX3CR1-deficient (CX3CR1(-/-)) mice exhibited exaggerated accumulation, histopathological alterations, neutrophil recruitment, but not macrophage infiltration. Mice...
Monocytes and macrophages produce interleukin (IL)-10, an immunoregulatory cytokine a potent therapeutic tool for immune disorders. Augmentation of IL-10 production with concomitant reduction proinflammatory cytokines in vitro is attained by doubly stimulating the cells toll-like receptor ligand immunoglobulin (Ig)G complexes, response known as that regulatory (or alternatively activated/M2) macrophages. However, it has not been explored sufficiently how such could be exploited...
Abstract Dendritic cells (DC) play pivotal roles in the induction and regulation of both innate acquired immunity. DC express several cell‐surface immune inhibitory receptors. However, little is known about their potential immunoregulatory functions context T‐cell activation. Here we report that murine gp49B, a member immunoglobulin superfamily, harboring immunoreceptor tyrosine‐based motifs, expressed on downregulates cellular activity to prevent excessive activation T vitro vivo . Bone...
TLRs are distributed in their characteristic cellular or subcellular compartments to efficiently recognize specific ligands and initiate intracellular signaling. Whereas recognizing pathogen-associated lipids proteins localized the cell surface, nucleic acid-sensing expressed endosomes lysosomes. Several endoplasmic reticulum (ER)-resident known regulate trafficking of compartments, thus playing important roles initiation innate immune responses. In this study, we show that an ER-resident...
Abstract Plasma cells (PCs) acquiring long lifespans in the bone marrow (BM) play a pivotal role humoral arm of immunological memory. The PCs reside special BM niche and produce antibodies against past-encountered pathogens or vaccine components for time. In BM, cysteine-X-cysteine (CXC) chemokine receptor type 4 (CXCR4)-expressing myeloid such as dendritic are attracted to held by CXC ligand 12 (CXCR12)-secreting stromal cells, where survival is supported soluble factors IL-6 APRIL (a...
Abstract AbstractImmune homeostasis is critically regulated by the balance between activating and inhibitory receptors expressed on various immune cells such as T B lymphocytes, myeloid cells. The play a fundamental role in checkpoint pathway, thus maintaining peripheral tolerance. We recently found that expression of leukocyte immunoglobulin-like receptor (LILR)B4, an member human LILR family, augmented auto-antibody-producing plasmablasts/plasma systemic lupus erythematosus (SLE) patients....
Abstract Bee venom (BV) induces skin inflammation, characterized by erythema, blisters, edemas, pain and itching. Although BV has been found to have an inhibitory effect on toll-like receptors (TLRs), we here show that enhances keratinocyte responses polyinosinic-polycytidylic acid [poly(I:C)], a ligand for TLR3. Our results revealed the enhanced TLR activity was primarily induced secretory phospholipase A2 (sPLA2), component of (BV-sPLA2). PLA2 mediates hydrolysis membrane phospholipids...
Abstract Murine gp49, a 49-kDa type I transmembrane glycoprotein, is member of the Ig-like receptors expressed on surface cells involved in natural immunity such as mast cells, NK and macrophages. The two major subtypes, gp49A gp49B, are encoded by different genes adjacent to each other. gp49B contains an immunoreceptor tyrosine-based inhibitory motif its cytoplasmic region known function molecule. In contrast, does not harbor any specific for signal transduction, nor has physiological role...
Kawasaki disease (KD) is an acute, systemic vasculitis syndrome that occurs in children. The clinical symptoms and epidemiologic features of KD strongly suggest triggered by unidentified infectious agents genetically predisposed patients. In addition, a number studies have described the role B cells development KD. To obtain mechanistic insight into humoral immune response B-lineage patients, we examined peripheral blood antibody secreting (ASCs) inhibitory immunoreceptors,...
The significance of a unique inhibitory Fc receptor for IgG, FcγRIIB (RIIB), in the prevention spontaneous production autoantibodies remains controversial, due mainly to fact that RIIB locus is adjacent autoimmune-related SLAM harboring genes coding signaling lymphocyte activation molecules, making it difficult isolate effect deletion from gene-targeted mice. Our objective was determine influence on development autoimmune diseases and compare with potentially pathogenic SLAM. We established...
A therapeutic preparation of polyclonal human IgG, i.e., intravenous immunoglobulin (IVIg), has been employed to treat several inflammatory and autoimmune disorders. B cells are supposed be a target IVIg, but the molecular mechanism is elusive because lack suitable experimental system. To gain an insight into beneficial effect IVIg on cells, we first established setting in which modulates murine cell function vitro, then aimed at identifying mechanistic features level. Here show that...