A5028067252- Reproductive tract infections research
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- Clostridium difficile and Clostridium perfringens research
- Biofuel production and bioconversion
- Urinary Tract Infections Management
- Microbial Metabolic Engineering and Bioproduction
- Cervical Cancer and HPV Research
- DNA Repair Mechanisms
- Syphilis Diagnosis and Treatment
- Bacterial Genetics and Biotechnology
- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- Microtubule and mitosis dynamics
- Plant and fungal interactions
- Glycosylation and Glycoproteins Research
- Plant Virus Research Studies
- RNA Research and Splicing
- RNA modifications and cancer
- Pediatric health and respiratory diseases
- Vibrio bacteria research studies
- Reproductive System and Pregnancy
Heinrich Heine University Düsseldorf
2016-2025
Institute of Virology of the Slovak Academy of Sciences
2023
University Hospital Cologne
2023
Ruhr University Bochum
2023
Robert Koch Institute
2023
Düsseldorf University Hospital
2023
Genomics (United Kingdom)
2016
Justus-Liebig-Universität Gießen
1991-2005
Salzburger Landeskliniken
2005
Charité - Universitätsmedizin Berlin
2005
The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches required to systematically determine their function. A total 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with precise deletion one 2026 ORFs (more than one-third the genome). Of deleted ORFs, 17 percent essential for viability rich medium. phenotypes more 500 assayed parallel. strains, 40 showed quantitative growth...
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has attracted much attention as a tool to study number of biological processes. This describes use GFP vital reporter molecule for localization and expression studies in Saccharomyces cerevisiae. Construction vectors which allow N- or C-terminal fusion gfp gene interest allowed generation proteins whose subcellular was followed by fluorescence microscopy living yeast cells. Analysis three unknown open reading frames...
Through a screen designed to isolate novel fission yeast genes required for chromosome segregation, we have identified mal3+. The mal3-1 mutation decreased the transmission fidelity of nonessential minichromosome and altered sensitivity microtubule-destabilizing drugs. Sequence analysis revealed that 35-kD Mal3 is member an evolutionary conserved protein family. Its human counterpart EB-1 was in interaction with tumour suppressor APC. able substitute complete loss mal3+ gene product...
Attachment and uptake into host cells are pivotal steps in the life cycle of Chlamydiaceae , a family obligate intracellular pathogens. Chlamydia trachomatis (Ctr) possesses nine polymorphic membrane proteins (Pmps), which have been shown to be crucial for adhesion internalization. However, host-cell molecules involved so far remained unknown. Here, we show that fragment Ctr PmpD, forms high-molecular-weight oligomers solution adheres epithelial cells, also binds secreted clusterin (sCLU),...
Chlamydia pneumoniae is an important respiratory pathogen. Recently, its presence has been demonstrated in atherosclerotic lesions. In this study, we characterized C. pneumoniae-mediated activation of endothelial cells and enhanced expression adhesion molecules followed by subsequent rolling, adhesion, transmigration leukocytes (monocytes, granulocytes). These effects were blocked mAbs against and/or leukocyte (beta1 beta2 integrins). Additionally, different signal transduction pathways...
Saccharomyces cerevisiae centromeres have a characteristic 120-base-pair region consisting of three distinct centromere DNA sequence elements (CDEI, CDEII, and CDEIII). We generated series 26 CEN mutations in vitro (including 22 point mutations, 3 insertions, 1 deletion) tested their effects on mitotic chromosome segregation by using new vector system. The yeast transformation pYCF5 was constructed to introduce wild-type mutant DNAs onto large, linear fragments which are mitotically stable...
A virus-encoded protease that cleaves after multiple basic amino acid residues has been implicated in the processing of flavivirus polyprotein. Recently, a computer search which might form active site led to suggestion amino-terminal segment NS3 protein represents serine protease. To examine this possibility we constructed an mRNA encodes polyprotein with signal sequence derived from influenza virus haemagglutinin, followed by West Nile includes non-structural (NS) proteins NS2A, NS2B and...
Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three DNA elements CDEI, CDEII, and CDEIII. We isolated 125-bp CEN6 fragment (named ML CEN6) containing only these assayed it completely separated its chromosomal context on circular minichromosomes large linear chromosome fragment. The results show that this itself sufficient for complete mitotic meiotic centromere functions.
Chlamydiae sp. are obligate intracellular pathogens that cause a variety of diseases in humans. Adhesion the infectious elementary body to eukaryotic host cell is pivotal step chlamydial pathogenesis. Here we describe characterization members polymorphic membrane protein family (Pmp), largest (with up 21 members) unique Chlamydiaceae. We show yeast cells displaying Pmp6, Pmp20 or Pmp21 on their surfaces, beads coated with recombinant proteins, adhere human epithelial cells. A hallmark Pmp...
The bacterial pathogens Chlamydia trachomatis and C. pneumoniae are obligate intracellular parasites, cause a number of serious diseases, can infect various cell types in humans. Chlamydial infections probably initiated by binding the outer membrane protein OmcB to host glycosaminoglycans (GAGs). Here, we show that all nine members polymorphic (Pmp) family mediate adhesion human epithelial endothelial cells. Importantly, exposure infectious particles soluble recombinant Pmps blocks...
Infection of mammalian cells by the strictly intracellular pathogens Chlamydiae requires adhesion and internalization infectious Elementary Bodies (EBs). The components latter step were unknown. Here, we identify Chlamydia pneumoniae Pmp21 as an invasin EGFR its receptor. Modulation surface expression evokes correlated changes in EB adhesion, infectivity. Ectopic EGFR-negative hamster leads to binding beads EBs, thus boosting infection. EB/Pmp21 invasion epithelial results activation EGFR,...
Chlamydia pneumoniae, an obligate intracellular human pathogen, causes a number of respiratory diseases. We explored the role conserved OmcB protein in C. pneumoniae infections, using yeast display technology. (i) Yeast cells presenting were found to adhere epithelial cells. (ii) Pre-incubation with heparin, but not other glycosaminoglycans (GAGs), abrogated adhesion. (iii) Pre-treatment target heparinase inhibited adherence, and GAG-deficient CHO cell lines failed bind yeast. (iv) A...
Infection of human cells by the obligate intracellular bacterium Chlamydia trachomatis requires adhesion and internalization infectious elementary body (EB). This highly complex process is poorly understood. Here, we characterize Ctad1 (CT017) as a new adhesin invasin from C. serovar E. Recombinant (rCtad1) binds to via two bacterial SH3 domains located in its N-terminal half. Pre-incubation host with rCtad1 reduces subsequent infectivity bacteria. Interestingly, protein-coated latex beads...
The assembly of the lipid-linked core oligosaccharide Glc3Man9GlcNAc2, substrate for N-linked glycosylation proteins in endoplasmic reticulum (ER), is catalyzed by different glycosyltransferases located at membrane ER. We report on identification and characterization ALG12 locus encoding a novel mannosyltransferase responsible addition α-1,6 mannose to dolichollinked Man7GlcNAc2. biosynthesis highly branched follows an ordered pathway which ensures that only completely assembled transferred...
Chlamydia pneumoniae is an important human intracellular pathogen; however, the pathogenesis of C. infection poorly understood, and bacterial adherence mechanism to host cells unknown. This study examined role glycosaminoglycans (GAGs) in adhesion eukaryotic cells. Heparin heparan sulfate were found inhibit attachment epithelial Reduction infectivity resulted from binding heparin organism. Enzymatic removal moieties cell surface led a marked decrease infectivity. Mutant CHO lines that...
The activation of primary human airway epithelial cells (HAECs) and the bronchial cell line BEAS-2B by Chlamydia pneumoniae, an important respiratory pathogen, was characterized. A time-dependent enhanced release interleukin (IL)-8 prostaglandin-E(2) increased expression adhesion molecule intercellular molecule-1 (ICAM-1), followed subsequent transepithelial migration polymorphonuclear neutrophils (PMN), were also demonstrated. PMN could be blocked anti-ICAM-1 monoclonal antibody (MAb) but...