A5064822079- Spinal Cord Injury Research
- Nerve injury and regeneration
- Bone Tissue Engineering Materials
- Nerve Injury and Rehabilitation
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Neuroscience of respiration and sleep
- Parkinson's Disease Mechanisms and Treatments
- Pluripotent Stem Cells Research
- Additive Manufacturing and 3D Printing Technologies
- Dental materials and restorations
- Advanced Drug Delivery Systems
- Neurological disorders and treatments
- Laser-Ablation Synthesis of Nanoparticles
- Titanium Alloys Microstructure and Properties
- Pain Mechanisms and Treatments
- Respiratory Support and Mechanisms
- Neurogenetic and Muscular Disorders Research
- Coronary Interventions and Diagnostics
- Cardiac Arrest and Resuscitation
- Neonatal and fetal brain pathology
- Cardiovascular Health and Disease Prevention
University of Minho
2013-2024
Abstract Background Parkinson’s disease (PD) is a chronic neurodegenerative condition that characterized by motor symptoms as result of dopaminergic degeneration, particularly in the mesostriatal pathway. However, recent years, greater number clinical studies have focused on emergence non-motor PD patients, consequence damage mesolimbic and mesocortical networks, their significant impact quality life patients. Herein, we performed thorough behavioral analysis including motor, emotional...
Abstract Adipose tissue‐derived stem cells (ASCs) have been shown to assist regenerative processes after spinal cord injury (SCI) through their secretome, which promotes several mechanisms, such as inducing axonal growth, reducing inflammation, promoting cell survival, and vascular remodeling, thus ultimately leading functional recovery. However, while systemic delivery (e.g., i.v. [intravenous]) may cause off‐target effects in different organs, the local administration has low efficiency...
Traumatic spinal cord injury (SCI) causes dramatic disability and dysfunction in the motor, sensory autonomic systems. The severe inflammatory reaction that occurs after SCI is strongly associated with further tissue damage. As such, immunomodulatory strategies have been developed, aimed at reducing inflammation, but also shaping immune response order to protect, repair promote regeneration of spared neural tissue. One those promising intraspinal administration cytokine interleukin-4 (IL-4)...
Abstract Background Alterations in the immune system are a complication of spinal cord injury (SCI) and have been linked to an excessive sympathetic outflow lymphoid organs. Still unknown is whether these peripheral changes also contribute for deleterious inflammatory response mounted at injured cord. Methods We analyzed different molecular outputs splenic signaling first 24 h after thoracic compression SCI. effect ablating innate spleen injury. Results found that norepinephrine (NE) levels...
Multi-material Ti6Al4V cellular structures impregnated with 45S5 bioactive glass were designed and produced using Selective Laser Melting (SLM), an additive manufacturing technique, combined Press Sintering focusing on load bearing components like hip implants. These to combine mechanical properties promote bone ingrowth into the structure as material (45S5) is being absorbed replaced by newly formed bone. The influence of these design some physical chemical aspects that drive response was...
Spinal cord injury (SCI) leads to dramatic impairments of motor, sensory, and autonomic functions affected individuals. Following the primary injury, there is an increased release glutamate that excitotoxicity further neuronal death. Therefore, modulating seems be a promising target promote neuroprotection during acute phase injury. In this study, we evaluated therapeutic effect FDA approved antiepileptic drug (levetiracetam-LEV), known for binding synaptic vesicle protein SV2A in brain...
Introduction The inflammatory response after spinal cord injury (SCI) is an important contributor to secondary damage. Infiltrating macrophages can acquire a spectrum of activation states, however, the microenvironment at SCI site favors macrophage polarization into pro-inflammatory phenotype, which one reasons why transplantation has failed. Methods In this study, we investigated therapeutic potential secretome for recovery. We effect in vitro using peripheral and CNS-derived neurons human...
Abstract Electroactive smart materials play an important role for tissue regenerative applications. Poly(vinylidene fluoride) (PVDF) is a specific subtype of piezoelectric electroactive material that generates electrical potential upon mechanical stimulation. This work focuses on the application PVDF films neural differentiation. Human precursor cells (hNPCs) are cultured poled and non‐poled β‐PVDF with or without pre‐coating step poly‐ d ‐lysine laminin (PDL/L). Subsequently, hNPCs...
The failure of axons to regenerate after a spinal cord injury (SCI) remains one the greatest challenges in neuroscience. initial mechanical trauma is followed by secondary cascade, creating hostile microenvironment, which not only permissive regeneration but also leads further damage. One most promising approaches for promoting axonal maintain levels cyclic adenosine monophosphate (cAMP), specifically phosphodiesterase-4 (PDE4) inhibitor expressed neural tissues. Therefore, our study, we...
Cervical spinal cord trauma represents more than half of the injury (SCI) cases worldwide. Respiratory compromise, as well severe limb motor deficits, are among main consequences cervical lesions. In present work, a Gellan Gum (GG)-based hydrogel modified with GRGDS peptide, together Adipose tissue-derived Stem/Stromal Cells (ASCs) and Olfactory Ensheathing (OECs), was used therapeutic strategy after C2 hemisection SCI in rats. Hydrogel or cells alone, control group without treatment, were...
Over 90% of chronic pain (CP) patients receive opioids-based treatments, which led to a public health crisis with lasting impacts on social and economic wellbeing based opioid addiction. Opioids act through activation μ (MOR), δ (DOR), κ (KOR) receptors, are broadly differentially distributed throughout the brain. Chronic consumption leads brain changes such as alterations neurotransmission, dendritic branching, spine density, well an increase in apoptosis. To overcome opioid-related issues,...
The low regeneration potential of the central nervous system (CNS) represents a challenge for development new therapeutic strategies neurodegenerative diseases, including spinocerebellar ataxias. Spinocerebellar ataxia type 3 (SCA3)-or Machado-Joseph disease (MJD)-is most common dominant ataxia, being mainly characterized by motor deficits; however, SCA3/MJD has complex and heterogeneous pathophysiology, involving many CNS brain regions, contributing to lack effective therapies. Mesenchymal...
In this study, Selective Laser Melting (SLM) was used to produce mono-material Ti64Al4V- and NiTi-cubic cellular structures with an open-cell size wall thickness of 500 μm 100 μm, respectively. Bioactive beta-tricalcium phosphate (βTCP) polymer poly-ether-ether ketone (PEEK) were fill the produced open-cells, thus creating multi-material components. These characterized in vitro terms cell viability, adhesion, differentiation mineralization. Also, bio-tribological experiments performed...
Citalopram is a selective serotonin reuptake inhibitor, and although widely used as an antidepressant, this drug has also demonstrated interesting repairing properties leading to motor recovery pathology amelioration in animal models of stroke degeneration. Here, we tested the efficacy both 7-day 8-week citalopram treatment contusive spinal cord injury (SCI) rat model. A combination behavioral tests, histological serum cytokine analysis was assess overall recovery. Despite promoting mild...
Abstract The Spinal Cord Injury (SCI) pathophysiology is highly complex, contributing to a poor prognosis and lack of effective treatments. Previously, we demonstrated that Roflumilast (Rof), leads functional recovery when tested in SCI contusion model. However, it unlikely Rof treatment on its own could fully restore the spinal cord. Therefore, our objective was scrutinize synergistic effects combining with neuroprotective approaches. Herein two therapies, firstly, combined Levetiracetam...
Abstract Numerical simulation of carotid artery bifurcation is essential for understanding blood flow dynamics in this complex region, aiding identifying patterns associated with stress points and atherosclerosis risk. This information valuable developing preventive therapeutic strategies vascular diseases, as well optimizing medical devices surgical interventions. Simulations offer insights into at the bifurcation, advancing biomedical research clinical applications. Engineers use...