A5048840478- Cancer Cells and Metastasis
- Endometrial and Cervical Cancer Treatments
- Cancer Genomics and Diagnostics
- Estrogen and related hormone effects
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- TGF-β signaling in diseases
- Endometriosis Research and Treatment
- Microtubule and mitosis dynamics
- Phagocytosis and Immune Regulation
- Genetic factors in colorectal cancer
- Ovarian cancer diagnosis and treatment
- Extracellular vesicles in disease
- Cancer Research and Treatments
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Sarcoma Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Cancer Treatment and Pharmacology
- Cancer-related molecular mechanisms research
- Prostate Cancer Treatment and Research
- MicroRNA in disease regulation
- Fibroblast Growth Factor Research
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
Instituto de Investigación Sanitaria de Santiago
2015-2024
Servicio Gallego de Salud
2015-2024
Universidade de Santiago de Compostela
2015-2024
Centro de Investigación Biomédica en Red de Cáncer
2018-2024
Hebron University
2007-2023
Institut Curie
2003-2023
Universidade da Coruña
2020-2023
Complexo Hospitalario Universitario A Coruña
2009-2020
Instituto de Salud Carlos III
2020
Complejo Hospitalario Universitario de Santiago
2010-2018
Abstract Endometrial cancer (EC) is the most frequent of invasive tumors female genital tract. Although usually detected in its initial stages, a 20% patients present with advanced disease. To date, no characterized molecular marker has been validated for diagnosis EC. In addition, new methods prognosis and classification EC are needed to combat this deadly We thus aimed identify markers evaluate their validity on endometrial aspirates. Gene expression screening 52 carcinoma samples series...
Wnt signaling is essential for tissue homeostasis and its dysregulation causes cancer. ligands trigger by activating Frizzled receptors (FZDRs), which belong to the G-protein coupled receptor superfamily. However, mechanisms of G protein activation in remain controversial. In this study, we demonstrate that FZDRs activate proteins non-canonical via Dishevelled-binding protein, Daple. Daple contains a Gα-binding (GBA) motif, activates Gαi an adjacent domain directly binds FZDRs, thereby...
In the present study we investigated prognostic value of Circulating Tumour Cells (CTC) and their utility for therapy monitoring in non-small cell lung cancer (NSCLC). A total 43 patients newly diagnosed with NSCLC were prospectively enrolled. Blood samples obtained before 1st, 2nd 5th cycles chemotherapy analyzed using CellSearch technology. Both CTC CTC-related objects (not morphological standard or broken epithelial cells) counted. At baseline 18 (41.9%) positive intact count 10 (23.2%)...
About 20% of patients diagnosed with endometrial cancer (EC) are considered high-risk unfavorable prognosis. In the framework European Network for Individualized Treatment in EC (ENITEC), we investigated presence and phenotypic features Circulating Tumor Cells (CTC) patients. CTC isolation was carried out peripheral blood samples from 34 patients, ranging Grade 3 Stage IB to IV carcinomas recurrences, 27 healthy controls using two methodologies. Samples were subjected EpCAM-based...
Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from primary tumor into bloodstream and, concordantly, presence these Circulating Tumor Cells (CTC) is associated with a poor prognosis. In this work, molecular characterization CTC mCRC patients was approached, aim understanding their biology and improving clinical utility in management patients. For this, EpCAM-based immunoisolation combined whole transcriptome amplification hybridization onto cDNA...
Remodeling targeted tissues for reception of tumor cells metastasizing from primary lesions is a consequence communication between the and environment that governs metastasis. This study describes novel approach aims to disrupt process metastasis by interfering with this intense dialogue. Proteomics adhesion assays identified exosomes purified ascitic fluid ovarian cancer patients (n = 9) as intermediaries cell attachment. A capture device was fabricated embedding onto 3D scaffold...
Abstract The interaction between circulating tumor cells (CTC) and endothelial during extravasation is a critical process metastatic colonization, but its mechanisms remain poorly characterized. Here we report that the luminal side of liver blood vessels contains fibronectin deposits are enriched in mice bearing primary tumors also present from human livers affected with metastases. Cancer attached to via talin1, major component focal adhesions. Talin1 depletion impaired cancer cell adhesion...
In migrating cells, force production relies essentially on a polarized actomyosin system, whereas the spatial regulation of contraction and substrate contact turnover involves complex cooperation between microtubule (MT) actin filament networks (Goode, B.L., D.G. Drubin, G. Barnes. 2000. Curr. Opin. Cell Biol., 12:63–71). Targeting capture MT plus ends at cell periphery has been described, but whether or not minus these MTs are anchored centrosome is known. Here, we show that release short...
Abstract BACKGROUND Several studies have demonstrated the usefulness of monitoring an RNA transcript in urine, such as PCA3, for prostate cancer (PCa) diagnosis. PCa screening would benefit from additional biomarkers higher specificity and could be used conjunction with prostate‐specific antigen (PSA) testing, order to better determine biopsy candidates. METHODS We urine sediments after massage (PM) 215 consecutive patients, who presented biopsy. tested whether G‐protein coupled receptor...
Circulating tumor cells (CTCs), proposed as major players in cancer dissemination, have demonstrated clinical prognostic significance several types. However, their predictive value remains unclear. Here we evaluated the utility of six CTC markers (tissue specific and epithelial to mesenchymal transition transcripts) both tools metastatic colorectal (mCRC) patients. CTCs were immunoisolated from blood 50 mCRC patients at baseline 4 16 weeks after treatment onset. Expression levels GAPDH,...
Zebrafish (Danio rerio) is a model organism that has emerged as tool for cancer research, being the second most common cause of death after cardiovascular disease humans in developed world. useful xenotransplantation human cells and toxicity studies different chemotherapeutic compounds vivo. Compared to murine model, zebrafish faster, can be screened using high-throughput methods lower maintenance cost, making it possible affordable create personalized therapies. While several cell...
Microtubules are specifically and efficiently visualized with the new fluorescent taxoids 7-O-[N-(4′-fluoresceincarbonyl)-L-alanyl]taxol (FLUTAX) 7-O-[N-(4′-tetramethylrhodaminecarbonyl)-L-alanyl]taxol (ROTAX). Similarly to taxol, FLUTAX ROTAX able drive inactive GDP-liganded tubulin into microtubule assembly. One molecule of binds per αβ-tubulin dimer assembled, competing taxol for same binding site an eightfold smaller relative affinity. FLUTAX-induced elongation is markedly...
Abstract Endometrial carcinoma is the most common gynecological malignant disease in industrialized countries. Two clinicopathological types of endometrial have been described, based on estrogen relation and grade: endometrioid (EEC) non-EEC (NEEC). Some molecular events that occur during development characterized, showing a dualistic genetic model for EEC NEEC. However, bases tumorigenesis are not clearly elucidated. In present work, we attempted to identify new genes could trigger cell...
Epithelial ovarian cancer is the most lethal gynecological malignancy and fifth leading cause of deaths in women Western world. ETS transcription factors are known to act as positive or negative regulators expression genes that involved various biological processes, including those control cellular proliferation, differentiation, apoptosis, tissue remodeling, angiogenesis transformation. ETV5 belongs PEA3 subfamily. subfamily members able activate proteases, matrix metalloproteinases...
Endometrial carcinomas, the most common malignant tumour of female genital tract, are usually diagnosed at an early stage with uterine‐confined disease and overall favourable prognosis. However, up to 20% endometrial carcinomas will end in recurrent disease, associated a drop survival representing major clinical challenge. Management this group risk patients relies on robust biomarkers that may predict which relapse. For this, we performed proteomic analysis comparing primary lesions...