A5030973249- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- RNA modifications and cancer
- Bladder and Urothelial Cancer Treatments
- Advanced Breast Cancer Therapies
- Erythrocyte Function and Pathophysiology
- Cancer-related molecular mechanisms research
- Hedgehog Signaling Pathway Studies
- Protein Degradation and Inhibitors
- Microfluidic and Bio-sensing Technologies
- Cancer, Hypoxia, and Metabolism
- Microtubule and mitosis dynamics
- Cancer Treatment and Pharmacology
- Genomics and Chromatin Dynamics
- Genetic Syndromes and Imprinting
- Cancer and Skin Lesions
- Gene expression and cancer classification
- Cancer, Stress, Anesthesia, and Immune Response
- Inflammatory Biomarkers in Disease Prognosis
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Head and Neck Cancer Studies
Centro de Investigación Biomédica en Red de Cáncer
2018-2025
Instituto de Investigación Sanitaria de Santiago
2014-2025
Roche (Spain)
2016-2024
Instituto de Salud Carlos III
2022
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2009-2017
Complejo Hospitalario Universitario de Santiago
2017
Servicio Gallego de Salud
2014-2016
Universidade de Santiago de Compostela
2015
Comunidad de Madrid
2014
Universidad Complutense de Madrid
2012
Liquid biopsy offers unique opportunities for low invasive diagnosis, real-time patient monitoring and treatment selection. The phenotypic molecular profile of circulating tumor cells (CTCs) can provide key information about the biology cells, contributing to personalized therapy. CTC isolation is still challenging, mainly due their heterogeneity rarity. To overcome this limitation, a microfluidic chip label-free CTCs from peripheral blood was developed. This device, CROSS chip, captures...
Remodeling targeted tissues for reception of tumor cells metastasizing from primary lesions is a consequence communication between the and environment that governs metastasis. This study describes novel approach aims to disrupt process metastasis by interfering with this intense dialogue. Proteomics adhesion assays identified exosomes purified ascitic fluid ovarian cancer patients (n = 9) as intermediaries cell attachment. A capture device was fabricated embedding onto 3D scaffold...
Missense mutations in TP53 gene promote metastasis human tumours. However, little is known about the complete loss of function p53 tumour metastasis. Here we show that squamous cell carcinomas generated by specific ablation Trp53 mouse epidermis are highly metastatic. Biochemical and genome-wide mRNA miRNA analyses demonstrated metastases associated with early induction epithelial-mesenchymal transition (EMT) deregulated expression primary Increased miR-21 was observed undifferentiated,...
Bladder cancer is a highly prevalent human disease in which retinoblastoma (Rb) pathway inactivation and epigenetic alterations are common events. However, the connection between these two processes still poorly understood. Here, we show that vivo of all Rb family genes mouse urothelium sufficient to initiate bladder development. The characterization tumors revealed multiple molecular features cancer, including activation E2F transcription factor subsequent Ezh2 expression several signaling...
Circulating tumor cell (CTC) enumeration has emerged as a powerful biomarker for the assessment of prognosis and response to treatment in metastatic breast cancer (MBC). Moreover, clinical evidences show that CTC-cluster counts add prognostic information CTC enumeration, however, their significance is not well understood, more are needed. We aim evaluate value longitudinally collected single CTCs CTC-clusters heterogeneous real-world cohort 54 MBC patients. Blood samples were at baseline...
The catalytic activity of GDP/GTP exchange factors (GEFs) is considered critical to maintain the typically high Rho GTPases found in cancer cells. However, large number them has made it difficult pinpoint those playing proactive, nonredundant roles tumors. In this work, we have investigated whether GEFs Vav subfamily exert such specific skin cancer. Using genetically engineered mice, show here that Vav2 and Vav3 favor cooperatively initiation promotion phases Transcriptomal profiling...
We have analyzed the role of thyroid hormone receptors (TRs) in epidermal homeostasis. Reduced keratinocyte proliferation is found interfollicular epidermis mice lacking binding isoforms TRα1 and TRβ (KO mice). Similar results were obtained hypothyroid animals, showing important liganded TRs proliferation. In addition, KO animals display decreased hyperplasia response to 12-O-tetradecanolyphorbol-13-acetate. Both receptor play overlapping functional roles skin because individually or also...
Background: Circulating tumor cells (CTC) have relevance as prognostic markers in breast cancer. However, the functional properties of CTCs or their molecular characterization not been well-studied. Experimental models indicate that only a few can survive circulation and eventually metastasize. Thus, it is essential to identify these surviving capable forming such metastases. Methods: We isolated viable from 50 peripheral blood samples obtained 35 patients with advanced metastatic cancer...
Head and neck squamous cell carcinoma (HNSCC) is a common human neoplasia with poor prognosis survival that frequently displays Akt overactivation. Here we show mice displaying constitutive activity (myrAkt) in combination Trp53 loss stratified epithelia develop oral cavity tumors phenocopy HNSCC. The myrAkt lesions, making it possible model of dysplasia. malignant conversion these which hampered due to the induction premature senescence, achieved by subsequent ablation gene same cells vivo....
Triple‐negative breast cancer (TNBC) is characterized by high rates of metastasis and no available molecular targets. CTCs derived xenografts (CDX) have demonstrated to be a promising tool for understanding biology. In our study, CDX from TNBC patient was developed the first time. After characterization, WNT signaling found as main mechanism related with this tumor biology potential markers were identified subsequently validated in patients. cohort levels MELK expression associated poorer...
Background: Circulating tumor cells (CTCs) and CTC-clusters are pivotal in the metastatic process of breast cancer (BC). Owing to their low frequency, models replicating biology should provide a robust platform for investigating molecular mechanisms driving metastasis identifying new biomarkers. We established characterized CTC-derived cell model from mouse xenograft explore its behavior profile, which allowed us investigate expression prognostic significance set genes associated with...
Metastatic breast cancer (BC) is the main cause of cancer-related mortality in women worldwide. HR + /HER2- BC patients are treated with endocrine therapy (ET), but therapeutic resistance common. The combination cyclin-dependent kinase 4/6 inhibitors (CDK4/6i) ET was approved for metastatic and extended median progression-free survival to 24 months. This not always effective, every patient, ultimately occurs, underlying mechanisms remain unclear. To address this gap, we explored circulating...
A critical step in the metastatic cascade is survival of circulating tumor cells (CTCs) within bloodstream. Although interactions between CTCs and various hematopoietic have been described, role red blood (RBCs) remains underexplored. This study investigated RBCs from breast lung cancer patients, revealing significant phenotypic functional changes cells, unlike with healthy donors. Tumor cell patient-derived RBC co-cultures increased attachment induced morphological changes. RBC-primed...
Traditionally, studies to address the characterization of mechanisms promoting tumor aggressiveness and progression have been focused only on primary analyses, which could provide relevant information but limitations really characterize more aggressive population. To overcome these limitations, circulating cells (CTCs) represent a noninvasive valuable tool for real-time profiling disseminated cells. Therefore, aim present study was explore value CTC enumeration identify markers associated...
Metastasis is the primary cause of death for most breast cancer (BC) patients who succumb to disease. During hematogenous dissemination, circulating tumor cells interact with different blood components. Thus, there are microenvironmental and systemic processes contributing regulation. We have recently published that red (RBCs) accompany prognostic value in metastatic BC patients. RBC alterations related several diseases. Although principal known role gas transport, it has been assigned...
The study of circulating tumor cells (CTCs) has a huge clinical interest in advance and metastatic breast cancer patients. However, many approaches are biased by the use epithelial markers, which underestimate non-epithelial CTCs phenotypes. enumeration provides valuable prognostic information; however, molecular characterization could be best option to monitor patients throughout disease since it may provide more relevant information physicians. In this work, we aimed at enumerating...
The epidermal specific ablation of Trp53 gene leads to the spontaneous development aggressive tumors in mice through a process that is accelerated by simultaneous Rb gene. Since alterations p53-dependent pathway are common hallmarks aggressive, poor prognostic human cancers, these mouse models can recapitulate molecular features some malignancies. To evaluate this possibility, expression microarray analysis was performed samples. display increased cell cycle and chromosomal instability...
Circulating tumour cells (CTCs) play a key role in the metastasis process, as they are responsible for micrometastasis and valuable tool monitoring patients real-time. Moreover, efforts to develop new strategies CTCs isolation characterisation, translation of into clinical practice needs overcome limitation associated with sole use Epithelial Cell Adhesion Molecule (EpCAM) expression purify this cell subpopulation. rare events blood believed represent epithelial population from primary...
Breast cancers of the luminal B subtype are frequent tumors with high proliferation and poor prognosis. Epigenetic alterations have been found in breast biological fluids. We aimed to profile cell-free DNA (cfDNA) methylome metastatic cancer (LBBC) patients using an epigenomic approach discover potential noninvasive biomarkers. Plasma cfDNA was analyzed Infinium MethylationEpic array a cohort 14 women, including LBBC nontumor controls. The methylation levels tissue samples were validated...
Abstract Background Increased ST6Gal I activity has been associated with the α(2,6)sialylation enhancement of membrane glycoconjugates observed in metastatic colorectal carcinomas (CRC). Siaα(2,6)Galβ(1,4)GlcNAc sequence, known as CDw75, is a sialylated carbohydrate determinant generated by I. This epitope reported to be progression gastric and tumours, hence there are only few conclusive studies date. Methods By radioisotopic techniques we evaluated healthy, transitional tumour tissues from...
Continuous cell renewal in mouse epidermis is at the expense of a pool pluripotent cells that lie well defined niche hair follicle known as bulge. To identify mechanisms controlling stem homeostasis, we developed strategy to isolate adult bulge mice and define their transcriptional profile. We observed large number transcripts are underexpressed when compared non-stem cells. Importantly, majority these downregulated genes involved cycle. Using bioinformatics tools, identified E2F...
Background: Circulating tumor cells (CTC) have relevance as prognostic markers in breast cancer. However, the functional properties of CTCs or their molecular characterization not been well-studied. Experimental models indicate that only a few can survive circulation and eventually metastasize. Thus, it is essential to identify these surviving capable forming such metastases. Methods: We isolated viable from 50 peripheral blood samples obtained 35 patients with advanced metastatic cancer...