A5003887595- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Ovarian cancer diagnosis and treatment
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Metastasis and carcinoma case studies
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Advanced Breast Cancer Therapies
- Cancer Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Bone health and treatments
- Lung Cancer Treatments and Mutations
- Ovarian function and disorders
- Pregnancy and preeclampsia studies
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Lung Cancer Research Studies
- Advanced Biosensing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
Essen University Hospital
2016-2025
University of Duisburg-Essen
2015-2024
National Center for Tumor Diseases
2019-2023
Klinik für Frauenheilkunde
2003-2022
Qiagen (Germany)
2018-2019
University Medical Center Hamburg-Eppendorf
2006-2019
Heidelberg University
2014-2019
University Hospital Heidelberg
2016-2019
Ludwig-Maximilians-Universität München
2011-2019
University of Oslo
2019
The persistence of circulating tumor cells (CTC) in breast cancer patients might be associated with stem cell like which have been suggested to the active source metastatic spread primary tumors. Furthermore, these also may undergo phenotypic changes, known as epithelial-mesenchymal transition (EMT), allows them travel site metastasis formation without getting affected by conventional treatment. Here we evaluated 226 blood samples 39 during a follow-up palliative chemo-, antibody - or...
The presence of circulating tumor cells (CTC) in breast cancer might be associated with stem cell-like which have been suggested to the active source metastatic spread primary tumors. Furthermore, able disseminate and metastasize, CTC must perform epithelial-mesenchymal transition (EMT). We studied expression three EMT markers cell marker ALDH1 from 502 patients. Data were correlated disseminated (DTC) bone marrow (BM) clinicopathological data patients.A total 2 × 5 ml blood was analyzed for...
Abstract Introduction The role of circulating tumor cells (CTCs) in blood primary breast cancer patients is still under investigation. We evaluated the incidence CTCs blood, we correlation between and disseminated (DTCs) bone marrow (BM), characterized for expression HER2, estrogen receptor (ER) progesterone (PR). Methods Blood 431 with were analyzed EpCAM, MUC1 HER2 transcripts AdnaTest BreastCancer ™ (AdnaGen AG, Germany). Expression ER PR was assessed an additional RT-PCR. BM aspirates...
Abstract Introduction There is a multitude of assays for the detection circulating tumor cells (CTCs) but very limited number studies comparing clinical relevance results obtained with different test methods. The DETECT trial metastatic breast cancer patients was designed to directly compare prognostic impact two commercially available CTC that are prominent representatives immunocytochemical and RT-PCR based technologies. Methods In total, 254 were enrolled in this prospective multicenter...
Extracellular vesicles (EVs) have been discussed as a diagnostic tool for minimal residual disease (MRD) evaluation in breast cancer (BC) addition to the analysis of circulating tumor cells (CTCs). Therefore, we investigated EV levels surrogate markers monitoring and prediction prognosis primary, non-metastatic, locally advanced BC patients. EVs were enriched from blood samples patients before after neoadjuvant chemotherapy (NACT) healthy females. marker expression was performed sizes...
Abstract BACKGROUND Platinum resistance constitutes one of the most recognized clinical challenges for ovarian cancer. Notably, detection primary tumor-based excision repair cross-complementation group 1 (ERCC1) protein by immunohistochemistry was recently shown to be inaccurate prediction platinum resistance. On basis previous finding that circulating tumor cells (CTC) in blood cancer patients are prognostically significant, and given our hypothesis negative prognostic impact CTC may arise...
Abstract Expression of the non-classical human leukocyte antigen-G (HLA-G) promotes cancer progression in various malignancies including epithelial ovarian (EOC). As single nucleotide polymorphisms (SNPs) HLA-G 3′ untranslated region (UTR) regulate expression, we investigated 3′UTR haplotypes arranged by SNPs healthy controls (n = 75) and primary EOC patients 79) determined soluble (sHLA-G) levels. Results were related to clinical status outcome. Although haplotype frequencies similar...
Bispecific T-cell engager (BiTE) are a class of bispecific single-chain antibodies that can very effectively redirect cytotoxic T cells for killing tumor target cells. Here, we have assessed the in vivo efficacy one representative, called bscEp-CAMxCD3, with specificity tumors overexpressing epithelial cell adhesion molecule (Ep-CAM) human xenograft models. Cells colon carcinoma line SW480 were mixed at 1:1 ratio unstimulated peripheral mononuclear cells, s.c. injected nonobese...
<h3>Objectives:</h3> Disseminated tumor cells (DTCs) in the bone marrow (BM) were shown to be of prognostic significance gynecological cancers. Bone aspiration is less accepted by patients compared with blood drawing. In this pilot study, we applied AdnaTest BreastCancer based on immunomagnetic enrichment, targeting common antigens epithelial cancers, followed multiplex reverse transcriptase-polymerase chain reaction for selection and detection circulating (CTCs) 122 ovarian cancer at...
Abstract Background As cell-free circulating DNA exists predominantly as mono- and oligonucleosomes, the focus of current study was to examine interplay nucleosomes, DNA, proteases caspases in blood patients with benign malignant breast diseases. Methods The concentrations nucleosomes well protease caspase activities were measured serum disease (n = 20), primary cancer (M0, n 31), metastatic (M1, 32), healthy individuals 28) by PicoGreen, Cell Death Detection ELISA, Protease Fluorescent Kit...
The number of unintentionally childless couples is increasing as more seek to conceive for the first time in third or fourth decade woman's life. Determination ovarian reserve an essential component infertility assessment. Anti-Müllerian-Hormone (AMH) seems be most reliable predictor reserve. In this study we analyzed AMH a cohort pregnant women without fertility impairment determine age-dependent decline and possible fluctuations during pregnancy postpartum. A total 554 healthy aged 16 47...
// Issam Chebouti 1, 4 , Sabine Kasimir-Bauer Paul Buderath Pauline Wimberger 2, 3, Siegfried Hauch 5 Rainer Kimmig and Jan Dominik Kuhlmann 1 Department of Gynecology Obstetrics, University Hospital Essen, 45147 Germany 2 Medical Faculty Carl Gustav Carus, Technische Universität Dresden, 01307 3 National Center for Tumor Diseases (NCT), Partner Site 69120 Heidelberg, German Cancer Consortium (DKTK), Dresden Research (DKFZ), QIAGEN, 40724 Hilden, Correspondence to: Kuhlmann, email:...
The expression of HER2, estrogen (ER) and progesterone (PR) receptor can change during the course disease in breast cancer (BC). Therefore, reassessment these markers at time progression might help to optimize treatment decisions. In this context, characterization circulating tumor cells (CTCs) could be relevance since metastatic tissue may difficult obtain for repeated analysis. Here we compared HER2/ER/PR profiles primary tumors, metastases CTCs.Ninety-six patients with BC from seven...
Patients with breast cancer (BC) undergoing neoadjuvant chemotherapy (NACT) may experience metastatic relapse despite achieving a pathologic complete response. We analyzed patients BC before and after NACT for disseminated tumor cells (DTCs) in the bone marrow(BM); comprehensively characterized circulating (CTCs), including stem cell-like CTCs (slCTCs), blood to prove effectiveness of treatment on these cells; correlated findings response therapy, progression-free survival (PFS), overall...
// Lydia Giannopoulou 1 , Issam Chebouti 2 Kitty Pavlakis 3 Sabine Kasimir-Bauer Evi S. Lianidou Analysis of Circulating Tumor Cells Laboratory, Laboratory Analytical Chemistry, Department University Athens, Campus, 15771, Greece Gynecology and Obstetrics, Hospital Essen, Duisburg-Essen, D-45122, Germany Pathology Department, IASO Women's Hospital, Marousi, 15123, Correspondence to: Lianidou, email: lianidou@chem.uoa.gr Keywords: circulating tumor DNA, RASSF1A, high-grade serous ovarian...
Liquid biopsies are discussed to provide surrogate markers for therapy stratification and monitoring. We compared messenger RNA (mRNA) profiles of circulating tumor cells (CTCs) extracellular vesicles (EVs) in patients with metastatic breast cancer (MBC) estimate their utility management.Blood was collected from 35 hormone receptor-positive/HER2-negative MBC at the time disease progression 2 consecutive staging points. CTCs were isolated 5 mL blood by positive immunomagnetic selection, EVs 4...
Introduction: Response to platinum-based therapy is a major prognostic factor in epithelial ovarian cancer (EOC) and reliable biomarkers are urgently needed identify patients at high risk. Since ligands of the Programmed Death Receptor-1 (PD-L1 PD-L2) play crucial role within tumor microenvironment for tumorigenesis, we investigated levels sPD-L1 sPD-L2 liquid biopsies serum samples, correlated results with clinical status, presence circulating cells (CTCs) disease outcome primary EOC...