A5083099939- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Pancreatic and Hepatic Oncology Research
- TGF-β signaling in diseases
- Colorectal Cancer Treatments and Studies
- Protease and Inhibitor Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Cancer Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Melanoma and MAPK Pathways
- Food Allergy and Anaphylaxis Research
- Radiopharmaceutical Chemistry and Applications
- Gastric Cancer Management and Outcomes
- Immunotherapy and Immune Responses
- S100 Proteins and Annexins
- CAR-T cell therapy research
- Inflammatory Bowel Disease
- Radiomics and Machine Learning in Medical Imaging
- Vaccine Coverage and Hesitancy
- Autoimmune and Inflammatory Disorders
- Hepatitis B Virus Studies
- Liver physiology and pathology
Nordic Bioscience (Denmark)
2017-2024
Statens Serum Institut
2017-2024
University of Copenhagen
2013-2020
Public Health Agency of Canada
2016-2020
Biomark (United States)
2020
Essen University Hospital
2019
Universitätsmedizin Greifswald
2013
Health Canada
2012
University of New Hampshire
2003
Collagens are a primary component of the extracellular matrix and functional ligands for inhibitory immune receptor leukocyte-associated immunoglobulin-like (LAIR)-1. LAIR-2 is secreted protein that can act as decoy by binding collagen with higher affinity than LAIR-1. We propose collagens promote evasion interacting LAIR-1 expressed on cells, releases LAIR-1-mediated suppression. Analysis public human datasets shows collagens, have unique overlapping associations survival in certain tumors....
Abstract Cancer‐associated fibroblasts (CAFs) deposit and remodel collagens in the tumor stroma, impacting cancer progression efficacy of interventions. CAFs are focus new therapeutics with aim normalizing microenvironment. To do this, a better understanding CAF heterogeneity collagen composition is needed. In this study, we sought to profile expression at multiple levels goal identifying biomarkers. We investigated pattern various cell types subtypes publicly available single‐cell RNA...
<h3>Background</h3> Excessive extracellular matrix (ECM) remodeling and a reactive stroma can affect T-cell infiltration activity in the tumor hereby influence response to immune checkpoint inhibitors (ICI). In pursuit of finding biomarkers that predict treatment response, we evaluated association between serum collagen vimentin turnover outcomes metastatic melanoma patients treated with anti-CTLA-4 antibody ipilimumab (IPI). <h3>Methods</h3> Type III formation (PRO-C3), MMP-degraded type I,...
Introduction: Response to platinum-based therapy is a major prognostic factor in epithelial ovarian cancer (EOC) and reliable biomarkers are urgently needed identify patients at high risk. Since ligands of the Programmed Death Receptor-1 (PD-L1 PD-L2) play crucial role within tumor microenvironment for tumorigenesis, we investigated levels sPD-L1 sPD-L2 liquid biopsies serum samples, correlated results with clinical status, presence circulating cells (CTCs) disease outcome primary EOC...
Increased remodeling of the extracellular matrix in malignant tumors has been shown to correlate with tumor aggressiveness and a poor prognosis. This involves degradation original (ECM) deposition new tumor-supporting ECM. The main constituent ECM is collagen turnover mainly occurs sequential manner, where initial proteolytic cleavage insoluble fibers followed by cellular internalization large well-defined fragments for lysosomal degradation. However, despite extensive research field, lack...
A T-cell permissive tumor microenvironment, characterized by the presence of activated T cells and low fibrotic activity is crucial for response to immune checkpoint inhibitors (ICIs). Granzyme B has been shown promote migration through basement membrane degradation type IV collagen. In this study, we evaluated biomarker potential measuring granzyme B-mediated collagen (C4G) in combination with a fibroblast activation (PRO-C3) non-invasively identifying metastatic melanoma patients...
Background Immune checkpoint inhibitors that target the programmed cell death protein 1 (PD-1) receptor induce a response in only subgroup of patients with metastatic melanoma. Previous research suggests transforming growth factor beta signaling and collagen-rich peritumoral stroma (tumor fibrosis), may negatively interfere interaction between T cells tumor thereby contribute to resistance mechanisms by immune-exclusion, while increased infiltration M1-like macrophages enhances activity....
In the tumor microenvironment, extracellular matrix (ECM) has been recognized as an important part of cancer development. The dominant ECM proteins are 28 types collagens, each with a unique function in tissue architecture. Type XX collagen, however, is poorly characterized, and little known about its involvement cancer. We developed ELISA quantifying type named PRO-C20, using monoclonal antibody raised against C-terminus. PRO-C20 PRO-C1, targeting N-terminal pro-peptide I was measured sera...
Abstract A desmoplastic colorectal cancer stroma, characterized by excess turnover of the cancer-associated fibroblast derived collagens type III and VI, can lead to reduced drug-uptake poor treatment response. We investigated association between biomarkers collagen VI overall survival (OS) in patients with metastatic (mCRC). Serum samples were collected from 252 mCRC prior bevacizumab chemotherapy. concentrations reflecting formation (PRO-C3) (PRO-C6) degradation (C6M C6Mα3) determined...
Circulating fragments of type III collagen, measured by PRO-C3, has shown promising results as a tumor fibrosis biomarker. However, the fibrotic microenvironment consists many other collagens with diverse functions and unexplored biomarker potential. One example hereof is XXII collagen (COL22). In this study, we investigated potential COL22 measuring in serum. An ELISA, named PRO-C22, was developed two serum cohorts consisting patients various solid tumors (n = 220) healthy subjects 33)...
Abstract Altered extracellular matrix ( ECM ) remodeling is an important part of the pathology gastrointestinal GI disorders. In intestine, type XVI collagen (col‐16) plays a role in pathogenesis by affecting architecture and induce cell invasion. Measuring col‐16 serum may therefore have biomarker potential disorders such as colorectal cancer CRC ulcerative colitis UC ). The aim this study was to determine whether can serve for altered patients with . A monoclonal antibody raised against...
Non-invasive biomarkers for diagnosing and prognosing hepatocellular carcinoma (HCC) are urgently needed. Cirrhosis is present in 80-90% of HCC patients. characterized by deposition cross-linking collagens that have crucial roles initiation progression. We evaluated circulating cross-linked pro-peptides type III collagen (PC3X) as a diagnostic prognostic biomarker HCC.PC3X was measured ELISA plasma from patients with (n=79), cirrhosis (n=86), non-cirrhotic hepatitis-B infection (n=74)...
In the absence of a validated correlate protection or robust animal models for human tuberculosis, Mycobacterial growth inhibition assays (MGIAs) aim to assess vaccines ability inhibit mycobacterial in-vitro. We optimised reproducible murine splenocyte MGIA based on in-vitro infection with virulent Mycobacterium tuberculosis (M.tb) Erdman. identified viability as problem in state-of-art protocols, which can be improved by simple changes culture conditions (viability increase from 21% 46% at...
Abstract Biliary tract cancer (BTC) is characterized by a desmoplastic extracellular matrix (ECM). We tested the diagnostic and prognostic use of seven circulating biomarkers ECM remodeling: pro‐peptides type III collagen (PRO‐C3), VI (PRO‐C6) XI (PRO‐C11), metalloprotease (MMP) degraded (C3M) IV (C4M) fragments, granzyme B fragments (C4G) MMP citrullinated vimentin (VICM) marker macrophage activation. The study included 269 patients with all stages BTC 49 benign biliary diseases. Serum...
Introduction: Pancreatic ductal adenocarcinoma (PDAC) is characterized by a pronounced fibrotic tumor microenvironment, which impairs treatment response. Type I and V collagens are responsible for the densely packed fibrils in fibrosis environment. While role of major type collagen cancer well described, less known about minor collagen. Quantifying propeptides serum has been shown to have prognostic predictive value. In this study, we evaluated clinical utility measuring propeptide (PRO-C5)...
Vaccination plays an essential role in the prevention of infectious diseases and safeguarding public health. Family physicians serve as vital sources information guidance when it comes to counselling patients about benefits vaccination. have a strong understanding
Pancreatic ductal adenocarcinoma (PDAC) is a hard-to-treat cancer due to the collagen-rich (fibrotic) and immune-suppressed microenvironment. A major driver of this phenomenon transforming growth factor beta (TGF-β). TGF-β produced in an inactive complex with latency-associated protein (LAP) that can be cleaved by plasma kallikrein (PLK), hereby releasing active TGF-β. The aim study was evaluate LAP PLK as non-invasive biomarker for PDAC tumor fibrosis. An ELISA developed quantification...