A5067007005- Epigenetics and DNA Methylation
- Bladder and Urothelial Cancer Treatments
- Advanced Breast Cancer Therapies
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Genetic diversity and population structure
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Cancer-related gene regulation
- FOXO transcription factor regulation
- Multiple and Secondary Primary Cancers
- Cancer Cells and Metastasis
- Urinary and Genital Oncology Studies
- Marine Bivalve and Aquaculture Studies
- Advanced Proteomics Techniques and Applications
- Genomics and Phylogenetic Studies
- Mollusks and Parasites Studies
- Molecular Biology Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Cancer Research and Treatments
- vaccines and immunoinformatics approaches
- Prostate Cancer Treatment and Research
Galicia Sur Biomedical Foundation
2022-2025
Servicio Gallego de Salud
2022-2025
Center for Research in Molecular Medicine and Chronic Diseases
2018-2024
Universidade de Santiago de Compostela
2018-2024
Translational Research in Oncology
2024
University Hospital Complex Of Vigo
2023
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2012-2022
Centro de Investigación Biomédica en Red de Cáncer
2017-2022
Research Institute Hospital 12 de Octubre
2014-2018
Unidades Centrales Científico-Técnicas
2018
Purpose: Bladder cancer is a current clinical and social problem. At diagnosis, most patients present with nonmuscle-invasive tumors, characterized by high recurrence rate, which could progress to muscle-invasive disease metastasis. Bone morphogenetic protein (BMP)-dependent signaling arising from stromal bladder tissue mediates urothelial homeostasis promoting cell differentiation. However, the possible role of BMP ligands in still unclear.Experimental Design: Tumor normal 68 were...
Parallel evolution of similar phenotypes provides strong evidence for the operation natural selection. Where these contribute to reproductive isolation, they further support a role divergent, habitat-associated selection in speciation. However, observation pairs divergent ecotypes currently occupying contrasting habitats distinct geographical regions is not sufficient infer parallel origins. Here we show striking phenotypic divergence between populations rocky-shore gastropod, Littorina...
Bladder cancer (BC) is one of the most common cancers in western world and ranks as expensive to manage, due need for cystoscopic examination. BC shows frequent changes DNA methylation, several studies have shown potential utility urinary biomarkers by detecting epigenetic alterations voided urine. The aim this study develop a targeted bisulfite next-generation sequencing assay diagnose from urine with high sensitivity specificity. We defined 150 CpG loci biomarker panel cohort 86...
NOTCH signaling suppresses tumor growth and proliferation in several types of stratified epithelia. Here, we show that missense mutations NOTCH1 NOTCH2 found human bladder cancers result loss function. In murine models, genetic ablation the pathway accelerated tumorigenesis promoted formation squamous cell carcinomas, with areas mesenchymal features. Using cancer cells, determined stabilizes epithelial phenotype through its effector HES1 and, consequently, activity favors process...
Bladder cancer is a highly prevalent human disease in which retinoblastoma (Rb) pathway inactivation and epigenetic alterations are common events. However, the connection between these two processes still poorly understood. Here, we show that vivo of all Rb family genes mouse urothelium sufficient to initiate bladder development. The characterization tumors revealed multiple molecular features cancer, including activation E2F transcription factor subsequent Ezh2 expression several signaling...
Long non-coding RNAs (lncRNAs) have been claimed as key molecular players in gene expression regulation, being involved diverse epigenetic processes. They are aberrantly expressed various tumors, but their exact role bladder cancer is still obscure. We recently found a major of the Polycomb repression complex recurrence non-muscle-invasive cancer. Here, we report xpression Polycomb-related lncRNAs:antisense noncoding RNA INK4 locus (ANRIL) and HOX antisense intergenic (HOTAIR) these...
Bladder cancer (BC) is the fifth most common in world, being non-muscle invasive tumors (NMIBC) frequent. NMIBC shows a very high frequency of recurrence and, certain cases, tumor progression. The phosphatidylinositol 3-kinase (PI3K) pathway, which controls cell growth, tumorigenesis, invasion and drug response, frequently activated numerous human cancers, including BC, part through alterations PIK3CA gene. However, significance gene with respect to clinicopathological characteristics,...
Abstract Purpose: Bladder cancer is a clinical and social problem due to its high incidence recurrence rates. It frequently appears in elderly patients showing other medical comorbidities that hamper the use of standard chemotherapy. We evaluated activity CDK4/6 inhibitor as new therapy for unfit cisplatin (CDDP). Experimental Design: cell lines were tested vitro sensitivity inhibition. A novel metastatic bladder mouse model was developed used test vivo activity. Results: Cell sensitive...
Abstract Most cancers are characterized by the somatic acquisition of genomic rearrangements during tumour evolution that eventually drive oncogenesis. Here, using multiplatform sequencing technologies, we identify and characterize a remarkable mutational mechanism in human hepatocellular carcinoma caused Hepatitis B virus, which DNA molecules from virus inserted into genome causing dramatic changes its configuration, including non-homologous chromosomal fusions, dicentric chromosomes...
Bladder cancer (BC) is a highly prevalent disease, ranking fifth in the most common cancers worldwide. Various miRNAs have recently emerged as potential prognostic biomarkers cancer. The miR-200 family, which repressed epithelial-to-mesenchymal transition (EMT), multiple advanced cancers. However, its expression and function BC still poorly understood. Here we show that family displays increased expression, probably due to activation of specific oncogenic signaling pathways, reduced promoter...
Immune checkpoint inhibitors (ICI) have revolutionized cancer treatment, particularly in advanced non-small cell lung (NSCLC) and muscle-invasive bladder (MIBC). However, identifying reliable predictive biomarkers for ICI response remains a significant challenge. In this study, we analyzed real-world cohorts of NSCLC MIBC patients treated with as first-line therapy. Tumor samples underwent Whole Genome Sequencing (WGS) to identify specific somatic variants assess tumor mutational burden...
The proteomic changes occurring during speciation are fundamental to understand this process, though they have been rarely addressed until present. Therefore, we compared the proteome of two ecotypes (RB and SU) marine snail Littorina saxatilis, a case sympatric incomplete speciation, originated as byproduct adaptation distinct habitats. Thus, RB ecotype is able resist stresses desiccation temperature on upper shore, whereas SU defies strong physical disturbances due wave action. Qualitative...
Abstract Hybrid zones of ecologically divergent populations are ideal systems to study the interaction between natural selection and gene flow during initial stages speciation. Here, we perform an amplified fragment length polymorphism ( AFLP ) genome scan in parallel hybrid ecotypes marine snail L ittorina saxatilis , which is considered a model case for ecological Ridged‐Banded RB Smooth‐Unbanded SU adapted different shore levels microhabitats, although they present sympatric distribution...
Both clinical and experimental observations show that the skin is affected by thyroidal status. In hypothyroid patients epidermis thin alopecia common, indicating status might influence not only proliferation but also hair growth. We demonstrate here thyroid hormone receptors (TRs) mediate these effects of hormones on skin. Mice lacking TRα1 TRβ (the main binding isoforms) display impaired cycling associated to a decrease in follicular cell proliferation. This was observed mice, important...
In previous studies we described a decreased alpha-L-fucosidase activity in colorectal tumors, appearing as prognostic factor of tumoral recurrence. The aim this work was to extend the knowledge about tissue cancer by quantifying expression its encoding gene FUCA1 tumors and healthy mucosa. mRNA levels were measured RT-qPCR paired tumor normal mucosa tissues from 31 patients. For accuracy results, five candidate reference genes validated those samples. addition, selected matched samples...
Abstract Background The role of phenotypic plasticity is increasingly being recognized in the field evolutionary studies. In this paper we look at genetic determination versus plastic response by comparing protein expression profiles between two sympatric ecotypes adapted to different shore levels and habitats using two-dimensional maps. Results We compared qualitative quantitative differences pools both from environments (field laboratory conditions). results suggested that ecotype may...
// Javier Puente 1 , Nuria Laínez 2 Marta Dueñas 3, 4 María José Méndez-Vidal 5 Emilio Esteban 6 Daniel Castellano 4, 7 Mónica Martinez-Fernández Laura Basterretxea 8 Juan-Fita 9 Luis Antón 10 León 11 Julio Lambea 12 Begoña Pérez-Valderrama 13 Sergio Vázquez 14 Cristina Suarez 15 Xavier Garcia del Muro 16 Enrique Gallardo 17 Pablo Maroto 18 M Luz Samaniego 19 Beatriz Suárez-Paniagua 20 Julián Sanz 21 Jesús M. Paramio SOGUG (Spanish Oncology Genitourinary Group) Medical Department, Instituto...
The study of circulating tumor cells (CTCs) has a huge clinical interest in advance and metastatic breast cancer patients. However, many approaches are biased by the use epithelial markers, which underestimate non-epithelial CTCs phenotypes. enumeration provides valuable prognostic information; however, molecular characterization could be best option to monitor patients throughout disease since it may provide more relevant information physicians. In this work, we aimed at enumerating...