A5010517161- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Cutaneous lymphoproliferative disorders research
- T-cell and B-cell Immunology
- Viral-associated cancers and disorders
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Adrenal and Paraganglionic Tumors
- Cancer Immunotherapy and Biomarkers
- T-cell and Retrovirus Studies
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Acute Myeloid Leukemia Research
- IL-33, ST2, and ILC Pathways
- Vascular Tumors and Angiosarcomas
- Hormonal Regulation and Hypertension
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Asthma and respiratory diseases
- CNS Lymphoma Diagnosis and Treatment
- Cancer Mechanisms and Therapy
- Multiple Myeloma Research and Treatments
- Inflammatory Bowel Disease
Centro Nacional de Investigaciones Científicas
2005-2025
Spanish National Cancer Research Centre
2015-2024
Centro de Investigación del Cáncer
2015-2024
Centro de Investigación Biomédica en Red
2024
Centre for Biomedical Network Research on Rare Diseases
2015-2024
Centro de Investigación Biomédica en Red de Cáncer
2022
Tokai University
2019
Cancer Research Center
2015
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2015
Universitat Pompeu Fabra
2015
Abstract Objective In the sanroque mouse model of lupus, pathologic germinal centers (GCs) arise due to increased numbers follicular helper T (Tfh) cells, resulting in high‐affinity anti–double‐stranded DNA antibodies that cause end‐organ inflammation, such as glomerulonephritis. The purpose this study was examine hypothesis pathway could account for a subset patients with systemic lupus erythematosus (SLE). Methods An expansion Tfh cells is causal, and therefore consistent, component...
The transcription factor FOXP3 plays a key role in CD4(+)CD25(+) regulatory T cell function and represents specific marker for these cells. Despite its strong association with function, humans little is known about the frequency of cells that express protein nor distribution vivo. Here we report characterization seven anti-FOXP3 monoclonal antibodies enabling detection endogenous human by flow cytometry immunohistochemistry. Flow-cytometric analysis showed was expressed majority...
Abstract Purpose: Recent studies of Hodgkin's lymphoma (HL) have suggested that the presence regulatory T cells in reactive background may explain inhibition antitumoral host immune response observed these patients. This study aimed to assess relevance and CTLs present HL samples prognosis a series classic (cHL) Experimental Design: Expression granzyme B TIA-1 (markers for CTL) FOXP3 (a marker cells) were evaluated independently by immunohistochemistry tissue microarrays 257 cHL patients...
Pheochromocytomas (PCC) and paragangliomas (PGL) are genetically heterogeneous neural crest-derived neoplasms. Recently we identified germline mutations in a new tumor suppressor susceptibility gene, MAX (MYC-associated factor X), which predisposes carriers to PCC. How contribute PCC/PGL associated phenotypes remain unclear. This study aimed examine the prevalence phenotypic features of somatic PCC/PGL.We sequenced 1,694 patients with PCC or PGL (without other major genes) from 17...
Tumor microenvironment influences the behavior of follicular lymphoma (FL), although specific cell subsets involved are not well known. The aim this study was to determine impact programmed death 1 (PD-1) -positive inhibitory immunoregulatory lymphoid cells in clinicobiologic features and outcome patients with FL.We examined samples from 100 (53 men 47 women; median age, 54 years) at diagnosis, as 32 first relapse, a recently generated monoclonal antibody against PD-1. were quantified using...
Abstract The mechanisms triggering metastasis in pheochromocytoma/paraganglioma are unknown, hindering therapeutic options for patients with metastatic tumors (mPPGL). Herein we show by genomic profiling of a large cohort mPPGLs that high mutational load, microsatellite instability and somatic copy-number alteration burden associated ATRX / TERT alterations suitable prognostic markers. Transcriptomic analysis defines the signaling networks involved acquisition competence establishes gene...
Abstract The historical lack of preclinical models reflecting the genetic heterogeneity multiple myeloma (MM) hampers advance therapeutic discoveries. To circumvent this limitation, we screened mice engineered to carry eight MM lesions (NF-κB, KRAS, MYC, TP53, BCL2, cyclin D1, MMSET/NSD2 and c-MAF) combinatorially activated in B lymphocytes following T cell-driven immunization. Fifteen genetically diverse developed bone marrow (BM) tumors fulfilling pathogenesis. Integrative analyses ∼500...
Routine sections of normal and pathological samples fixed in 10 per cent buffered formalin or B5, including EDTA-decalcified bone-marrow biopsies, were tested with 61 antibodies following heating three different fluids: 0·01 m citrate buffer (pH 6·0), 0·1 Tris–HCl 8·0), 1 mm EDTA–NaOH solution 8·0). The underwent either cycles microwave treatment (5 min each) pressure cooking for 1–2 min. alkaline phosphatase/anti-alkaline phosphatase (APAAP) technique was used as the standard detection...
Cutaneous CD4 small/medium-sized pleomorphic T-cell lymphoma (CSTCL) is a cutaneous defined by predominance of small-to-medium-sized T cells, with favorable clinical course. Cases are also characterized the presence rich infiltrate reactive B cells. Recently, it has been reported that follicular helper cells (TFH cells) display distinct gene expression profile, positive for PD-1, CXCL13, and BCL-6. We report first time PD-1 other TFH cell markers in CSTCLs discuss its biologic significance....
HLA-F is a human non-classical MHC molecule. Recombinant heavy chain was refolded with β2-microglobulin to form stable complex. This complex used as an immunogen produce highly specific, high-affinity monoclonal antibody (FG1) that study directly the cellular biology and tissue distribution of HLA-F. has restricted pattern expression in tonsil, spleen, thymus. could be immunoprecipitated from B cell lines HUT-78, T line. binds TAP, but unlike classical class I molecules, undetected at...
Rare cases of peripheral T-cell lymphomas with follicular growth pattern (PTCL-F) have been recently reported, and their association t(5;9)(q33;q22) involving ITK SYK has suggested. However, the clinicopathologic aspects PTCL-F are poorly described normal cell counterpart this subgroup lymphoma is still unknown. Therefore, we analyzed pathologic, phenotypic, cytogenetic features a series 30 patients (range: 33 to 88 y) that showed histopathologic in at least 1 biopsy (n=30), either initial...
Disruption of the Krebs cycle is a hallmark cancer. IDH1 and IDH2 mutations are found in many neoplasms, germline alterations SDH genes FH predispose to pheochromocytoma/paraganglioma other cancers. We describe paraganglioma family carrying mutation MDH2, which encodes enzyme. Whole-exome sequencing was applied tumor DNA obtained from man age 55 years diagnosed with multiple malignant paragangliomas. Data were analyzed two-sided Student's t Mann-Whitney U tests Bonferroni correction for...
The microenvironment influences outcome in follicular lymphoma. Our hypothesis was that several immune cell subsets are important for disease and their individual prognostic importance should be demonstrable the same analysis competition with clinical factors.Seventy lymphoma patients extreme ("poor" "good" cases) were selected a population-based cohort of 197. None 37 good-outcome died from lymphoma, whereas all 33 poor-outcome succumbed <or=5 years. Furthermore, followed long time needed...
Background and Objectives In the present paper we report that SAP, an intracytoplasmic molecule is involved in cell signaling, immunohistologic marker for germinal center T cells paraffin-embedded tissue. We document its expression, also of PD-1 (another recently described to which a new antibody has been raised), normal neoplastic lymphoid tissue evaluate suggestion helper within centers human are origin angioimmunoblastic T-cell lymphoma (AITL), assess diagnostic value these two...
Background Plasmablastic lymphoma has recently come to be considered a distinct entity among mature B cell neoplasms, although the limits with diffuse large B-cell (DLBCL) need more accurately defined.Design and Methods Here we show results of an immunohistochemical study 35 cases plasmablastic compared set 111 conventional DLBCLs.Results Our demonstrate that use limited combination markers (PAX5&CD20, PRDM1/BLIMP1 XBP1s) enables identification immunophenotype highly characteristic...
NOTCH signaling suppresses tumor growth and proliferation in several types of stratified epithelia. Here, we show that missense mutations NOTCH1 NOTCH2 found human bladder cancers result loss function. In murine models, genetic ablation the pathway accelerated tumorigenesis promoted formation squamous cell carcinomas, with areas mesenchymal features. Using cancer cells, determined stabilizes epithelial phenotype through its effector HES1 and, consequently, activity favors process...
We have found that FOXP3 is an oligomeric component of a large supramolecular complex. Certain mutants with single amino acid deletions in the leucine zipper domain are associated X-linked autoimmunity-allergic dysregulation (XLAAD) and immunodysregulation, polyendocrinopathy enteropathy, (IPEX) syndrome humans. report deletion human XLAAD/IPEX patients within does not disrupt its ability to join larger protein complex, but eliminates homo-oligomerization as well heteromerization FOXP1. zinc...
The nodularity and presence of T-cell rosettes surrounding the neoplastic cells has been described as a defining feature nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). We have explored potential diagnostic value new marker (NAT105) that recognizes antigen PD-1 in series 152 cases diagnosed sclerosis lymphoma, mixed cellularity lymphocyte-rich classic NLPHL, T-cell/histiocyte-rich B-cell (T/HRBCL). All were immunostained with panel antibodies against CD10, bcl-6, CXCL13, CD57,...
Peripheral T-cell lymphomas (PTCLs) in western countries are uncommon tumors with unfavorable prognosis. They may be subclassified as anaplastic large-cell (ALCLs), angioimmunoblastic-T-cell (AITLs), or unspecified peripheral (PTCLs-U). It has recently been demonstrated that AITLs originate from germinal center follicular helper T cells (TFH), whereas the normal counterparts of other PTCLs remain essentially unknown. The aim this study was to establish whether PTCL subgroups also express TFH...