A5062990199- Cancer Cells and Metastasis
- Telomeres, Telomerase, and Senescence
- Pancreatic and Hepatic Oncology Research
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- FOXO transcription factor regulation
- Chemokine receptors and signaling
- Renal and related cancers
- Estrogen and related hormone effects
- Evolution and Genetic Dynamics
- Epigenetics and DNA Methylation
- Radiation Therapy and Dosimetry
- Cancer, Stress, Anesthesia, and Immune Response
- Bacterial Genetics and Biotechnology
- Evolutionary Game Theory and Cooperation
- Mechanisms of cancer metastasis
- Plant and animal studies
- Cancer, Hypoxia, and Metabolism
- Escherichia coli research studies
- Graphene and Nanomaterials Applications
- Protein Hydrolysis and Bioactive Peptides
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- S100 Proteins and Annexins
- Supramolecular Self-Assembly in Materials
Universität Ulm
2019-2024
University Hospital Ulm
2020-2023
Tata Memorial Hospital
2017-2018
Advanced Centre for Treatment, Research and Education in Cancer
2017
Indian Institute of Science Education and Research Pune
2016
Abstract Pancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate approximately 7–9%. The ineffectiveness anti-PDAC therapies is believed to be due existence subpopulation tumor cells known as stem (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant metastatic capacities. Herein, we describe 2D in vitro system for long-term enrichment pancreatic CSCs that amenable biological CSC-specific studies. By...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and metastatic malignancies worldwide. Migrating cancer stem cells (miCSCs) marked by CD133+CXCR4+ expression drives metastasis but lacks effective drug targets. Here, we show that activated pancreatic stellate secrete CXCR4 ligand CXCL12 to foster stemness, epithelial-to-mesenchymal transition (EMT), chemoresistance. Protein interaction network analyses links CXCL12/CXCR4 signaling axis downstream transcription factor...
To assess the role of telomerase activity and telomere length in pancreatic CSCs we used different CSC enrichment methods (CD133, ALDH, sphere formation) primary patient-derived cancer cells. We show that have higher longer telomeres than bulk tumor Inhibition activity, using genetic knockdown or pharmacological inhibitor (BIBR1532), resulted marker depletion, abrogation formation vitro reduced tumorigenicity vivo. Furthermore, identify a positive feedback loop between stemness factors...
Pre-operative progesterone treatment of breast cancer has been shown to confer survival benefits patients independent their receptor (PR) status. The underlying mechanism and the question whether such an effect can also be observed in PR negative cells remain resolved.We performed proteome profiling PR-positive PR-negative response using a phospho-kinase array platform. Western blotting was used validate results. Cell-based phenotypic assays were conducted assess progesterone.We found that...
The adsorption of cationic peptide JM21 onto different mesoporous silica nanoparticles (MSNs) from an aqueous solution was studied as a function pH. In agreement with the literature, highest loading degrees could be achieved at pH close to isoelectric point where peptide–peptide repulsion is minimum. However, mesopore size, geometry, and surface polarity all had influence on in terms both affinity maximum given This behavior largely explained by combination pH-dependent electrostatic...
Pancreatic ductal adenocarcinoma (PDAC) remains a devastating disease with very poor prognosis. At the same time, its incidence is on rise, and PDAC expected to become second leading cause of cancer-related death by 2030. Despite extensive work new therapeutic approaches, median overall survival only 6-12 months after diagnosis 5-year less than 7%. While pancreatic cancer particularly difficult treat, patients usually succumb not growth primary tumor, but metastasis; therefore, strategies...
Abstract Pancreatic cancer is a fatal disease and one of the most aggressive metastatic malignancies worldwide. The dissemination tumor cells prerequisite metastases correlates with loss epithelial differentiation acquisition migratory phenotype, hallmark malignant progression. Migrating stem (miCSCs) characterized by CD133+ CXCR4+ expression play pivotal role in formation, have been reported to form invasive front metastasis silico analysis showed that both CSCs miCSCs are significantly...
Pancreatic ductal adenocarcinoma (PDAC) remains a largely incurable cancer type. Its high mortality is attributed to the lack of efficient biomarkers for early detection combined with its metastatic properties. The aim our study was investigate role NF-κB signaling in development and metastasis PDAC. We used well-established KPC mouse model, and, through genetic manipulation, we deleted essential modulator (NEMO) pancreata mice. Interestingly, NEMO deletion altered differentiation status...
Abstract To date, it is still unclear how cancer stem cells (CSCs) regulate their stemness properties, and to what extent they share common features with normal cells. Telomerase regulation a key factor in cell maintenance. In this study, we investigate telomerase affects biology pancreatic ductal adenocarcinoma (PDAC), delineate the mechanisms by which activity CSC properties are linked. Using primary patient-derived cells, show that CSCs have higher longer telomeres than bulk tumor...
Abstract Little is known about the mechanisms that enable organisms to cope with unpredictable environments. To address this issue, we used replicate populations of Escherichia coli selected under complex, randomly changing Under four novel stresses had no correlation selection environments, individual cells significantly lower lag and greater yield compared controls. More importantly, there were outliers in terms growth, thus ruling out evolution population-based resistance. We also assayed...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer characterized by late diagnosis, lack of early symptoms and extensive metastasis. One the foremost reasons for such startling statistics presence subpopulation highly plastic stem-like cells within tumor called stem (CSCs). We have previously identified distinct subset these CSCs invasive front patient tumors. This subset, migrating (miCSCs), CD133+CXCR4+ expression determines metastatic phenotype pancreatic cancer....
PDAC ist eine der aggressivsten und invasivsten Tumorerkrankungen heutigen Zeit aktuell auf Platz vier krebsbedingten Todesursachen. Aufgrund sehr frühen Metastasierung, Therapieresistenz Rezidivneigung die Behandlung üblicherweise schwierig. Eine entscheidende Rolle in Tumorigenese Metastasierung spielen Tumorstammzellen (CSCs), welche einige stammzell-ähnliche Eigenschaften (z.B. unbegrenztes Selbsterneuerungs- Proliferationspotential) aufweisen. Diese Zellen zeigen auch hohe metabolische...
Pancreatic cancer is expected to become the 2nd leading cause of cancer-related deaths by 2030. One reasons for such startling statistics presence a subpopulation highly plastic stem-like cells within tumor. We have previously identified distinct subset these stem (CSCs) characterized CD133+CXCR4+ expression invasive front patient tumors, which determined metastatic phenotype pancreatic cancer. Development and investigation novel treatment regimens imperative specifically target eradicate...