A5081498212- Glioma Diagnosis and Treatment
- DNA Repair Mechanisms
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Cancer therapeutics and mechanisms
- Telomeres, Telomerase, and Senescence
- PARP inhibition in cancer therapy
- Histone Deacetylase Inhibitors Research
- Nanoparticle-Based Drug Delivery
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Proteoglycans and glycosaminoglycans research
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 detection and testing
- Epigenetics and DNA Methylation
- Axon Guidance and Neuronal Signaling
- Cholangiocarcinoma and Gallbladder Cancer Studies
- S100 Proteins and Annexins
Advanced Centre for Treatment, Research and Education in Cancer
2016-2025
Tata Memorial Hospital
2016-2025
Homi Bhabha National Institute
2017-2025
Jawaharlal Nehru University
2024-2025
Clinical Trials New Zealand
2021
Brigham and Women's Hospital
2009-2012
University of Zurich
2011
Boston Children's Hospital
2005-2009
Boston University
2007
Harvard University
2005-2007
Understanding of molecular events underlying resistance and relapse in glioblastoma (GBM) is hampered due to lack accessibility resistant cells from patients undergone therapy. Therefore, we mimicked clinical scenario an vitro cellular model developed five GBM grade IV primary patient samples two cell lines. We show that upon exposure lethal dose radiation, a subpopulation cells, innately survive transiently arrest G2/M phase via inhibitory pCdk1(Y15). Although arrested, these multinucleated...
Several non-invasive Raman spectroscopy-based assays have been reported for rapid and sensitive detection of pathogens. We developed a novel statistical model the RNA viruses in saliva, based on an unbiased selection set 65 spectral features that mostly attribute to moieties, with prediction accuracy 91.6% (92.5% sensitivity 88.8% specificity). Furthermore, minimize variability automate downstream analysis spectra, we GUI-based analytical tool "RNA Virus Detector (RVD)." This conceptual...
We previously showed the selective expression of chondroitin sulfate proteoglycans versican V0 and V1 in barrier tissues that impede migration neural crest cells during embryonic trunk development (Landolt, R. M., Vaughan, L., Winterhalter, K. H., Zimmermann, D. (1995) Development 212, 2303-2312). To test for an active involvement these isoforms guidance process, we have now established protocols to isolate intact quantities sufficient functional experiments. Using stripe choice assays,...
Bystander cells of the tumor microenvironment show evidence DNA damage and inflammation that can lead to their oncogenic transformation. Mediator(s) cell-cell communication brings about these pro-oncogenic pathologies has not been identified. We here cell-free chromatin (cfCh) released from dying cancer are key mediators trigger both in surrounding healthy cells. When human were cultured along with NIH3T3 mouse fibroblast cells, numerous cfCh emerged them rapidly entered into nuclei...
Notch pathway plays a complex role depending on cellular contexts: promotes stem cell maintenance or induces terminal differentiation in potential cancer-initiating cells; acts as an oncogene lymphocytes and mammary tissue growth-suppressive leukemia, liver, skin, head neck cancer. Here, we present novel clinical functional significance of NOTCH1 alterations early stage tongue squamous carcinoma (TSCC).We analyzed the signaling 68 TSCC primary tumor samples by whole exome transcriptome...
The uncommonness of gallbladder cancer in the developed world has contributed to generally poor understanding disease. Our integrated analysis whole exome sequencing, copy number alterations, immunohistochemical, and phospho‐proteome array profiling indicates ERBB2 alterations 40% early‐stage rare tumors, among an ethnically distinct population not studied before, that occurs through overexpression 24% ( n = 25) recurrent mutations 14% tumors 44); along with co‐occurring KRAS mutation 7%...
Early diagnosis of SARS-CoV-2 infected patients is essential to control the dynamics COVID-19 pandemic. We develop a rapid and accurate one-step multiplex TaqMan probe-based real-time RT-PCR assay, along with computational tool systematically analyse data. Our assay could detect limit 15 copies transcripts—based on experiments performed by spiking total human RNA in vitro synthesized viral transcripts. The was evaluated performing 184 validations for Nucleocapsid gene RNase P as an internal...
Abstract Background Residual disease of glioblastoma (GBM) causes recurrence. However, targeting residual cells has failed, due to their inaccessibility and our lack understanding survival mechanisms radiation therapy. Here we deciphered a cell–specific mechanism essential for GBM relapse. Methods Therapy resistant (RR) were captured from primary patient samples cell line models mimicking clinical scenario resistance. Molecular signaling resistance in RR was identified using RNA sequencing,...
System xc- (Sxc- ), a cystine-glutamate antiporter, is established as an interesting target for the treatment of several pathologies including epileptic seizures, glioma, neurodegenerative diseases, and multiple sclerosis. Erastin, sorafenib, sulfasalazine (SSZ) are few inhibitors Sxc- . However, its pharmacological inhibition with novel potent agents still very much required due to potential issues, example, potency, bioavailability, blood-brain barrier (BBB) permeability, current lead...
ABSTRACT Anthracyclines, topoisomerase II enzyme poisons that cause DNA damage, are the mainstay of acute myeloid leukemia (AML) treatment. However, acquired resistance to anthracyclines leads relapse, which currently lacks effective treatment and is poor survival in individuals with AML. Therefore, identification mechanisms underlying anthracycline remains an unmet clinical need. Here, using patient-derived primary cultures clinically relevant cellular models recapitulate AML, we have found...
An inability to discern resistant cells from bulk tumour cell population contributes poor prognosis in Glioblastoma. Here, we compared parent and recurrent generated patient derived primary cultures lines identify their unique molecular hallmarks. Although morphologically similar, different samples showed variable biological properties like proliferation radiation resistance. However, total RNA-sequencing revealed transcriptional landscape populations. These data suggest that global...
Leukemia is majorly treated by topoisomerase inhibitors that induce DNA double strand breaks (DSB) resulting in cell death. Consequently, modulation of DSB repair pathway renders leukemic cells resistant to therapy. As we do not fully understand the regulation acquired cells, targeting these has been a challenge. Here investigated early drug population (EDRP) and late (LDRP). We found doxorubicin induced equal DSBs parent EDRP cells; however, death only cells. Further analysis revealed...