A5056479445- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Pancreatic function and diabetes
- Regulation of Appetite and Obesity
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- PARP inhibition in cancer therapy
- DNA Repair Mechanisms
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Microtubule and mitosis dynamics
- Biochemical Analysis and Sensing Techniques
- Renal and related cancers
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Pancreatitis Pathology and Treatment
- TGF-β signaling in diseases
- Ferroptosis and cancer prognosis
- Renal cell carcinoma treatment
- Aldose Reductase and Taurine
- melanin and skin pigmentation
- Prenatal Substance Exposure Effects
- Cancer Research and Treatments
University Hospital Ulm
2018-2024
Universität Ulm
2019-2023
Klinik und Poliklinik für Psychosomatische Medizin und Psychotherapie
2020-2021
Centre de Recherche en Cancérologie de Lyon
2012-2017
Inserm
2006-2016
Centre National de la Recherche Scientifique
2012-2016
Université Claude Bernard Lyon 1
2006-2016
Centre Léon Bérard
2012-2016
École Normale Supérieure de Lyon
2015
Lyon College
2005
// Benjamin Le Calvé 1, 2, 3, 4, 14 , Audrey Griveau * David Vindrieux Raphaël Maréchal 5 Clotilde Wiel 4 Magali Svrcek 6, 7 Johann Gout Lamia Azzi 8 Léa Payen 9 Jérôme Cros 10, 11 Christelle de la Fouchardière 3 Pierre Dubus Guitton Laurent Bartholin Jean-Baptiste Bachet 12, 13 Bernard 1 Inserm U1052, Centre Recherche en Cancérologie Lyon, France 2 CNRS UMR5286, Léon Bérard, Université Department of Gastroenterology and Gastrointestinal Cancer Unit, Erasme Hospital, Libre Bruxelles, Belgium...
Organotypic cultures derived from pancreatic ductal adenocarcinoma (PDAC) termed cancer organoids (PDOs) recapitulate the primary and can be or metastatic biopsies. Although isolation culture of patient-derived were established several years ago, pros cons for individualized medicine have not been comprehensively investigated to date.We conducted a feasibility study, systematically comparing head-to-head xenograft tumor (PDX) PDX-derived by rigorous immunohistochemical molecular...
(ATM) is the most frequently mutated DNA damage response gene, involved in homologous recombination (HR), pancreatic ductal adenocarcinoma (PDAC).
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more one week. More 20 x 106 acinar cells can be obtained from a single pancreas. offers the possibility independently process as many 10 pancreases parallel. Because preserves architecture, this model is well suited studying physiology exocrine pancreas vitro contrast cell lines established tumors, which display genetic alterations resulting partial or...
Transforming growth factor beta (TGFβ) acts either as a tumor suppressor or an oncogene, depending on the cellular context and time of activation. TGFβ activates canonical SMAD pathway through its interaction with serine/threonine kinase type I II heterotetrameric receptors. Previous studies investigating TGFβ-mediated signaling in pancreas relied loss-of-function approaches ligand overexpression, effects acinar cells have so far remained elusive.
Real-time isolation, propagation, and pharmacotyping of patient-derived pancreatic cancer organoids (PDOs) may enable treatment response prediction personalization (PC) therapy. In our methodology, PDOs are isolated from 54 patients with suspected or confirmed PC in the framework a prospective feasibility trial. The drug single agents is determined by viability assay. Areas under curves (AUC) clustered for each drug, score developed combined regimens. Pharmacotyping profiles obtained 28...
The transcription accessory factor TIF1γ/TRIM33/RFG7/PTC7/Ectodermin functions as a tumor suppressor that promotes development and cellular differentiation. However, its precise function in cancer has been elusive. In the present study, we report TIF1γ inactivation causes cells to accumulate chromosomal defects, hallmark of cancer, due attenuations spindle assembly checkpoint post-mitotic checkpoint. deficiency also caused loss contact growth inhibition increased anchorage-independent vitro...
Personalized medicine in treating pancreatic ductal adenocarcinoma (PDAC) is still its infancy, albeit PDAC-related deaths are projected to rise over the next decade. Only recently, maintenance therapy with PARP inhibitor olaparib showed improved progression-free survival germline BRCA1/2-mutated PDAC patients after platinum-based induction for first time. Transferability of such a concept other DNA damage response (DDR) genes remains unclear. Here, we conducted placebo-controlled,...
Impaired DNA damage repair (DDR) is increasingly recognised as a hallmark in pancreatic ductal adenocarcinoma (PDAC). It estimated that around 14% of human PDACs harbour mutations genes involved DDR, including, amongst others, BRCA1/2, PALB2, ATM, MSH2, MSH6 and MLH1. Recently, DDR intervention by PARP inhibitor therapy has demonstrated effectiveness germline BRCA1/2-mutated PDAC. Extending this outcome to the significant proportion with somatic or beyond BRCA1/2 might be beneficial, but...
The aldo-keto reductase 1B7 (AKR1B7) encodes an aldose-reductase that has been reported as a detoxification enzyme until now. We have demonstrated AKR1B7 is differently expressed in various mouse white adipose tissues depending on their location. Its expression associated with higher ratio of preadipocytes vs. adipocytes. cells express did not contain lipid droplets, and the level akr1b7 was very low mature defined role adipogenesis using either primary cultures stromal (containing adipocyte...
NUPR1 (nuclear protein 1), also called P8 (molecular mass 8 kDa) or COM1 (candidate of metastasis is involved in the stress response and cancer progression. In present study, we investigated whether human expression was regulated by TGFβ (transforming growth factor β), a secreted polypeptide largely tumorigenesis. We demonstrate that activated at transcriptional level. show this activation mediated SMAD proteins, which are transcription factors specifically signalling superfamily members....
Transcriptional intermediary factor 1γ (TIF1γ; alias, TRIM33/RFG7/PTC7/ectodermin) belongs to an evolutionarily conserved family of nuclear factors that have been implicated in stem cell pluripotency, embryonic development, and tumor suppression. TIF1γ expression is markedly down-regulated human pancreatic tumors, Pdx1-driven Tif1γ inactivation cooperates with the KrasG12D oncogene mouse pancreas induce intraductal papillary mucinous neoplasms. In this study, we report aged Pdx1-Cre;...
The objectives of this study were to identify potential alterations in gene expression melanocortin‐4 receptor ( MC4‐R ), proopiomelanocortin POMC and Agouti‐related protein AgRP ) mouse hypothalamus under a chronic peripheral infusion leptin or at early (8 weeks) advanced (16 phases diet‐induced obesity. Control obesity mice 16 weeks high‐fat diet) either treated not with leptin. Metabolic features analyzed the genes interest was measured by quantitative reverse transcriptase‐PCR (RT‐qPCR)...
Background Many cancer cells express a major histocompatibility complex class I low/ programmed cell death 1 ligand positive (MHC-I lo /PD-L1 + ) surface profile. For immunotherapy, there is, thus, an urgent need to restore presentation competence of with defects in MHC-I processing/presentation combined immune interventions that tackle the tumor-initiated PD-L1/PD-1 signaling axis. Using pancreatic ductal adenocarcinoma (PDACCs) as model, we here explored if (and how) expression/processing...
Despite intensive research and progress in personalized medicine, pancreatic ductal adenocarcinoma remains one of the deadliest cancer entities. Pancreatic duct-like organoids (PDLOs) derived from human pluripotent stem cells (PSCs) or patient-derived (PDOs) provide unique tools to study early late stage dysplasia foster medicine. However, such advanced systems are neither rapidly nor easily accessible require an vivo niche tumor formation interaction with stroma. Here, establishment porcine...
Pancreatic ductal adenocarcinoma (PDAC) remains a devastating disease with very poor prognosis. At the same time, its incidence is on rise, and PDAC expected to become second leading cause of cancer-related death by 2030. Despite extensive work new therapeutic approaches, median overall survival only 6-12 months after diagnosis 5-year less than 7%. While pancreatic cancer particularly difficult treat, patients usually succumb not growth primary tumor, but metastasis; therefore, strategies...
Abstract Alk4 is a type I receptor that belongs to the transforming growth factor‐beta (TGF‐β) family. It takes part in signaling of TGF‐β ligands such as Activins, Gdfs, and Nodal had been demonstrated participate numerous mechanisms ranging from early embryonic development adult‐tissue homeostasis. Evidences indicate key regulator many processes, but little known about its adult tissues pathological conditions where mutations reported. Conventional deletion gene ( Acvr1b ) results...
Somatic cell reprogramming and tissue repair share relevant factors molecular programs. Here, Dickkopf-3 (DKK3) is identified as novel factor for organ regeneration using combined transcription-factor-induced RNA-interference techniques. Loss of Dkk3 enhances the generation induced pluripotent stem cells but does not affect de novo derivation embryonic cells, three-germ-layer differentiation or colony formation capacity liver pancreatic organoids. However, DKK3 expression levels in wildtype...
Abstract Pancreatic ductal adenocarcinoma (PDAC) still presents with a dismal prognosis despite intense research. Better understanding of cellular homeostasis could identify druggable targets to improve therapy. Here we propose RAD50-interacting protein 1 (RINT1) as an essential mediator in PDAC. In cohort resected PDAC, low RINT1 expression correlated significantly better survival. Accordingly, depletion caused severe growth defects vitro associated accumulation DNA double-strand breaks...