A5021056317- Hormonal Regulation and Hypertension
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Effects and risks of endocrine disrupting chemicals
- Adrenal Hormones and Disorders
- Growth Hormone and Insulin-like Growth Factors
- Receptor Mechanisms and Signaling
- Aldose Reductase and Taurine
- Biochemical Analysis and Sensing Techniques
- Stress Responses and Cortisol
- Toxic Organic Pollutants Impact
- Estrogen and related hormone effects
- Nutritional Studies and Diet
- Diet and metabolism studies
- Birth, Development, and Health
- Hormonal and reproductive studies
- melanin and skin pigmentation
- Sperm and Testicular Function
- Carcinogens and Genotoxicity Assessment
- Diabetes and associated disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Consumer Attitudes and Food Labeling
- RNA Research and Splicing
Inserm
2010-2021
Université Claude Bernard Lyon 1
2010-2021
Laboratoire CarMeN
2011-2021
Hospices Civils de Lyon
2011-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2012-2021
Institut National des Sciences Appliquées de Lyon
2014-2019
Lyon College
2005-2013
Institut National de la Recherche Agronomique
2013
Hôpital Debrousse
1996-2005
Immunology from Concept and Experiments to Translation
1994-2004
Background Dietary interventions are critical in the prevention of metabolic diseases. Yet, effects fatty fish consumption on type 2 diabetes remain unclear. The aim this study was to investigate whether a diet containing farmed salmon prevents or contributes insulin resistance mice. Methodology/Principal Findings Adult male C57BL/6J mice were fed control (C), very high-fat without with Atlantic fillet (VHF and VHF/S, respectively), Western (WD WD/S, respectively). Other VHF reduced...
Familial glucocorticoid deficiency (FGD) is an autosomal recessive disorder resulting from resistance to the action of ACTH on adrenal cortex. Affected individuals are deficient in cortisol and, if untreated, likely succumb hypoglycemia and/or overwhelming infection. Mutations receptor (MC2R) and melanocortin 2 accessory protein (MRAP), FGD types 1 respectively, account for approximately 45% cases.A locus chromosome 8 has previously been linked disease three families, but no underlying gene...
The regulation of ACTH receptor binding sites and mRNA by angiotensin II (A-II) was studied using cultured human adrenal fasciculata reticularis cells (HAC). These expressed two major transcripts 1.8 3.4 kb three minor ones 4, 7, 11 kb. increased the levels all these in a time- dose-dependent manner. At maximal concentration 10(-8) M, enhanced 21- 4-fold level number receptors per cell, respectively. Pretreatment HAC with A-II produced enhancement that associated an increase both...
In the present study we developed a model of diet-induced obesity (DIO) in male C57 BL/6J mice using an 8-wk high fat diet. This should better reflect physiology majority human obese patients than mouse genetic models with defects leptin or signaling. At end diet, DIO displayed increased weight (20%) and higher leptin, insulin, glucose, corticosterone plasma levels compared fed standard diet during same period. Moreover, they became resistant to central effect peripheral administration...
Basal plasma GH levels and the responses to an injection of 1 μg/kg 1–44(NH2) GHRH were determined on day 3 postnatally in 5 small gestational age (SGA) twin newborns their appropriate (AGA) co-twins, 10 SGA singleton 6 AGA newborns. The mean basal level was higher than infants but difference significant only for (p < 0.01). peak markedly increased compared 0.05 twins, p 0.01 singletons). Twelve re-tested at month had lower 0.001 In 21 17 infants, serum IGF-I, measured by RIA between 12 96...
Environmental contaminants are suspected to be involved in the epidemic incidence of metabolic disorders, food ingestion being a primarily route exposure. We hypothesized that life-long consumption high-fat diet contains low doses pollutants will aggravate disorders induced by obesity itself. Mice were challenged from preconception throughout life with containing commonly present (2,3,7,8-tetrachlorodibenzo-p-dioxin, polychlorinated biphenyl 153, diethylhexyl phthalate, and bisphenol A),...
The hereditary syndrome of unresponsiveness to ACTH is a rare autosomal recessive disorder characterized by low levels serum cortisol and high plasma ACTH. There no response exogenous Recent cloning the human receptor gene has enabled us study this in patients with glucocorticoid deficiency. By using PCR amplify coding sequence gene, we identified three mutations two unrelated patients. One mutation present homozygous form converted negatively charged Asp107, located third transmembrane...
Abstract The role of insulin-like growth factor I (IGF-I) on the specific function several steroidogenic cells has been recently reported. Since IGF-I is produced by tissues, we have investigated whether bovine adrenal secrete this peptide. Purification conditioned medium from incubated with [35S]methionine through affinity chromatography (monoclonal anti-IGF-I antibody), high pressure liquid chromatography, and polyacrylamide gel electrophoresis revealed a single band similar Mr as pure...
Transforming growth factor-beta 1 (TGF beta 1) has been reported to have a strong inhibitory effect on the specific function of adrenal cells several species. In present study, we examined effects TGF cultured human fasciculata-reticularis cells. alone had no ACTH receptor messenger ribonucleic acid (mRNA) levels and was unable reduce stimulatory its own receptor. However, enhanced angiotensin-II type mRNA binding sites. Treatment with increased significantly 3 beta-hydroxysteroid...
The maintenance of optimal steroidogenesis in adrenocortical cells primarily depends on the chronic action ACTH to promote synthesis various steroid-metabolizing cytochrome P-450 enzymes. In steroidogenic pathway, 17 alpha-hydroxylase (P-450(17) alpha) is a key enzyme controlling formation cortisol and androgens. Recently, we demonstrated that transforming growth factor-beta (TGF beta) potent inhibitor steroid production ovine cells. present study used polyclonal antibody P450(17) alpha...
Pollutants are suspected to contribute the etiology of obesity and related metabolic disorders. Apart from occupational exposure which concerns a subset chemicals, humans mostly exposed large variety all life-long at low doses. Food ingestion is major route it suggested that pollutants have worsened impact when combined with high-fat diet. In experimental studies described herein, we aimed add further evidence on food using recently set up model in mice fed high-fat/high-sucrose diet (HFSD)...
The maintenance of optimal steroidogenesis in adrenocortical cells primarily depends on the chronic action ACTH to promote synthesis various steroid metabolizing enzymes. In steroidogenic pathway, ratio 3β-hydroxysteroid dehydrogenase (3β-HSD) 17α-hydroxylase cytochrome P450 (P-45017α) plays a key role determining final products released by adrenal cells. differences these enzymes are particularly important when one considers zones and secretion zone-specific steroids. present study we have...
In the steroidogenic pathways present in gonads and adrenal cortex, 3β-hydroxysteroid dehydrogenase isomerase (3βHSD) is a key enzyme which controls formation of Δ4–3-ketosteroids from Δ5–3β-hydroxysteroids. Herein, we used an antibody against human placental 3βHSD rat testicular cDNA probe to study expression liver mRNA protein. Rat microsomal activity has been previously reported exhibit significant sex difference, with much higher male. We have shown age-dependent increase levels...