A5064901841- TGF-β signaling in diseases
- RNA modifications and cancer
- Pancreatic and Hepatic Oncology Research
- Renal and related cancers
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Genetic factors in colorectal cancer
- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Chemical Reactions and Isotopes
- Cancer-related gene regulation
- Melanoma and MAPK Pathways
- Ferroptosis and cancer prognosis
- Aluminum Alloy Microstructure Properties
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Technology Assessment and Management
- Amino Acid Enzymes and Metabolism
- Neuroendocrine Tumor Research Advances
- Long-Term Effects of COVID-19
- Infectious Encephalopathies and Encephalitis
- Chromatin Remodeling and Cancer
- Kruppel-like factors research
- Renal cell carcinoma treatment
Cancer Research UK Scotland Institute
2015-2023
Fordham University
2018-2021
Centre National de la Recherche Scientifique
1981-2020
Centre de Recherche en Cancérologie de Lyon
2008-2020
Inserm
2008-2020
Université Claude Bernard Lyon 1
1981-2020
Centre Léon Bérard
2008-2020
Switch
2020
Cancer Research UK
2017-2018
École Normale Supérieure de Lyon
2015
Inhibiting acute injury–induced senescence mediated by TGFβ signaling in regenerative epithelium improves liver regeneration.
Abstract Melanoma patients treated with oncogenic BRAF inhibitors can develop cutaneous squamous cell carcinoma (cSCC) within weeks of treatment, driven by paradoxical RAS/RAF/MAPK pathway activation. Here we identify frequent TGFBR1 and TGFBR2 mutations in human vemurafenib-induced skin lesions sporadic cSCC. Functional analysis reveals these ablate canonical TGFβ Smad signalling, which is localized to bulge stem cells both normal murine skin. MAPK hyperactivation (through Braf V600E or...
Abstract Right-sided (proximal) colorectal cancer (CRC) has a poor prognosis and distinct mutational profile, characterized by oncogenic BRAF mutations aberrations in mismatch repair TGFβ signalling. Here, we describe mouse model of right-sided colon driven loss epithelial TGFβ-receptor The proximal colonic tumours that develop this exhibit foetal-like progenitor phenotype ( Ly6a/Sca1 + ) and, importantly, lack expression Lgr5 its associated intestinal stem cell signature. These features are...
Inactivation of the Transforming Growth Factor Beta (TGFβ) tumor suppressor pathway contributes to progression Pancreatic Ductal AdenoCarcinoma (PDAC) since it is inactivated in virtually all cases this malignancy. Genetic lesions inactivating contribute pancreatic mouse models. Transcriptional Intermediary 1 gamma (TIF1γ) has recently been proposed be involved TGFβ signaling, functioning as either a positive or negative regulator pathway. Here, we addressed role TIF1γ carcinogenesis. Using...
Invariant natural killer T (iNKT) cells constitute a distinct subset of lymphocytes exhibiting important immune-regulatory functions. Although various steps their differentiation have been well characterized, the factors controlling development remain poorly documented. Here, we show that TGF-β controls program iNKT cells. We demonstrate signaling carefully and specifically orchestrates several cell development. In vivo, this multifaceted role involves concerted action different pathways...
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more one week. More 20 x 106 acinar cells can be obtained from a single pancreas. offers the possibility independently process as many 10 pancreases parallel. Because preserves architecture, this model is well suited studying physiology exocrine pancreas vitro contrast cell lines established tumors, which display genetic alterations resulting partial or...
Transforming growth factor beta (TGFβ) acts either as a tumor suppressor or an oncogene, depending on the cellular context and time of activation. TGFβ activates canonical SMAD pathway through its interaction with serine/threonine kinase type I II heterotetrameric receptors. Previous studies investigating TGFβ-mediated signaling in pancreas relied loss-of-function approaches ligand overexpression, effects acinar cells have so far remained elusive.
Recent studies have suggested increased plasticity of differentiated cells within the intestine to act both as intestinal stem (ISCs) and tumour-initiating cells. However, little is known processes that regulate this plasticity. Our previous work has shown activating mutations Kras or NF-κB pathway can drive dedifferentiation lacking Apc. To investigate process further, we profiled undergoing in vitro tumours generated from these vivo by gene expression analysis. Remarkably, no clear...
Abstract ECT2 is an activator of RHO GTPases that essential for cytokinesis. In addition, was identified as oncoprotein when expressed ectopically in NIH/3T3 fibroblasts. However, oncogenic activation resulted from N-terminal truncation, and such truncated proteins have not been found patients with cancer. this study, we observed elevated expression full-length protein preneoplastic colon adenomas, driven by increased mRNA abundance associated APC tumor-suppressor loss. Elevated levels were...
Abstract We generated a transgenic mouse strain (LSL‐TβRI CA ) containing latent constitutively active TGFβ type I receptor (TβRI/ALK5) by using knock‐in strategy into the X chromosome‐linked hypoxanthine phosphoribosyl‐transferase ( Hprt locus. Transgene expression, under control of ubiquitous CAG (human cytomegalovirus enhancer and chicken β‐actin) promoter, is repressed floxed transcriptional “Stop” (LSL, Lox‐Stop‐Lox). In presence cre‐recombinase, excised to allow TβRI transgene...
Clinical laboratories are adopting array genomic hybridization as a standard clinical test. A number of whole genome platforms available, but little is known about their comparative performance in context. We studied 30 children with idiopathic MR and both unaffected parents each child using Affymetrix 500 K GeneChip SNP arrays, Agilent Human Genome 244 oligonucleotide arrays NimbleGen 385 Whole-Genome arrays. also determined whether CNVs called on these were detected by Illumina Hap550...
The transcription accessory factor TIF1γ/TRIM33/RFG7/PTC7/Ectodermin functions as a tumor suppressor that promotes development and cellular differentiation. However, its precise function in cancer has been elusive. In the present study, we report TIF1γ inactivation causes cells to accumulate chromosomal defects, hallmark of cancer, due attenuations spindle assembly checkpoint post-mitotic checkpoint. deficiency also caused loss contact growth inhibition increased anchorage-independent vitro...
NUPR1 (nuclear protein 1), also called P8 (molecular mass 8 kDa) or COM1 (candidate of metastasis is involved in the stress response and cancer progression. In present study, we investigated whether human expression was regulated by TGFβ (transforming growth factor β), a secreted polypeptide largely tumorigenesis. We demonstrate that activated at transcriptional level. show this activation mediated SMAD proteins, which are transcription factors specifically signalling superfamily members....
Transcriptional intermediary factor 1γ (TIF1γ; alias, TRIM33/RFG7/PTC7/ectodermin) belongs to an evolutionarily conserved family of nuclear factors that have been implicated in stem cell pluripotency, embryonic development, and tumor suppression. TIF1γ expression is markedly down-regulated human pancreatic tumors, Pdx1-driven Tif1γ inactivation cooperates with the KrasG12D oncogene mouse pancreas induce intraductal papillary mucinous neoplasms. In this study, we report aged Pdx1-Cre;...
// Yang Gao 1 , David F. Vincent 3 Anna Jane Davis Owen J. Sansom Laurent Bartholin 2 and Qinglei Li Department of Veterinary Integrative Biosciences, College Medicine Biomedical Sciences, Texas A&M University, Station, TX, USA Centre de Recherche en Cancérologie Lyon, INSERM U1052, CNRS UMR5286, France Cancer Research UK Beatson Institute, Garscube Estate, Glasgow, United Kingdom Correspondence to: Li, email: Keywords : TGFβ, TGFBR1, tumor, ovary, mouse model Received April...
Abstract RAC1 activity is critical for intestinal homeostasis, and required hyperproliferation driven by loss of the tumour suppressor gene Apc in murine intestine. To avoid impact direct targeting upon we reasoned that indirect via RAC-GEFs might be effective. Transcriptional profiling deficient tissue identified Vav3 Tiam1 as key targets. Deletion these indicated while TIAM1 deficiency could suppress Apc- hyperproliferation, it had no tumourigenesis, VAV3 effect. Intriguingly, deletion...
AbstractAbstractPhases formed by isothermal diffusion in the Al–Zn–Sn ternary system have been studied at 270, 280, and 290°C for three couples (Zn–10Sn)–Al, (Zn–28Al)–Sn, (Al–10Sn)–Zn. The present work completes an earlier study using direct together with differential thermal analysis, provides better understanding of equilibria. Comparing results allows different possible paths to be proposed, microprobe analysis phases equilibrium gives detailed information on phase diagram.MST/378
Cellular senescence, a stable proliferation arrest, is induced in response to various stresses. Oncogenic stress-induced senescence (OIS) results blocked and constitutes fail-safe program counteracting tumorigenesis. The events that enable tumor benign senescent state escape from OIS become malignant are largely unknown. We show lysyl oxidase activity contributes the decision maintain senescence. Indeed, human epithelial cell constitutive expression of LOX or LOXL2 protein favored escape,...
// Lindsay C. Spender 1,9 , G. John Ferguson 1,10 Sijia Liu 4 Chao Cui Maria Romina Girotti 5 Gary Sibbet 1 Ellen B. Higgs 9 Morven K. Shuttleworth Tom Hamilton 2 Paul Lorigan 6 Michael Weller 7 David F. Vincent 3 Owen J. Sansom Margaret Frame 8 Peter ten Dijke Richard Marais and Gareth Inman Growth Factor Signalling Laboratory, The Beatson Institute for Cancer Research, Bearsden, Glasgow, United Kingdom Biological Services, Colorectal Wnt Signalling, Department of Molecular Cell Biology,...
Abstract The pro-tumourigenic role of epithelial TGFβ signalling in colorectal cancer (CRC) is controversial. Here, we identify a cohort born to be bad early-stage (T1) tumours, with aggressive features and propensity disseminate early, that are characterised by high cell-intrinsic signalling. In the presence concurrent Apc Kras mutations, activation rampantly accelerates tumourigenesis share transcriptional signatures those T1 human tumours predicts recurrence stage II CRC. Mechanistically,...
Uterine gland development, also known as adenogenesis, is a key uterine morphogenic process indispensable for normal function and fertility. Our earlier studies have reported that overactivation of TGFB receptor 1 (TGFBR1) in the mouse uterus using progesterone (Pgr)-Cre recombinase causes female infertility, defective decidualization, reduced formation, developmental milestone postnatal uterus. To understand mechanisms underpin disrupted formation mice with sustained activation TGFBR1, we...
Job satisfaction and organizational commitment are critical factors in retention of qualified experienced social workers. Palliative care organizations may struggle to retain workers who question if the organization's practices conflict with work values justice equity. Improving palliative workers' job can be a pathway keeping their jobs. Aims this study were explore how influences intention stay jobs, these associations mediated by satisfaction. A cross-sectional, survey design,...