A5047911400- Infectious Diseases and Mycology
- Myxozoan Parasites in Aquatic Species
- Actinomycetales infections and treatment
- Aquaculture disease management and microbiota
- Cancer-related molecular mechanisms research
- Veterinary Oncology Research
- Mycobacterium research and diagnosis
- Vector-Borne Animal Diseases
- Human-Animal Interaction Studies
- Genetics, Bioinformatics, and Biomedical Research
- T-cell and B-cell Immunology
- Radiomics and Machine Learning in Medical Imaging
- MicroRNA in disease regulation
- Contemporary Literature and Criticism
- Vector-borne infectious diseases
- Cancer Cells and Metastasis
- CAR-T cell therapy research
- Microbial infections and disease research
- Cell Adhesion Molecules Research
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- AI in cancer detection
- Violence, Religion, and Philosophy
- Vascular Tumors and Angiosarcomas
- Ferroptosis and cancer prognosis
University of Sassari
2022-2025
Roche (Switzerland)
2020-2025
Cancer Research UK Scotland Institute
2013-2021
University of Warwick
2021
Institute of Cancer Research
2015
MRC University of Glasgow Centre for Virus Research
2011-2014
Medical Research Council
2011-2014
Universidad de Zaragoza
2012
University of Glasgow
2007-2009
Royal College of Physicians and Surgeons of Glasgow
2009
MicroRNA deregulation is frequent in human colorectal cancers (CRCs), but little known as to whether it represents a bystander event or actually drives tumor progression vivo. We show that miR-135b overexpression triggered mice and humans by APC loss, PTEN/PI3K pathway deregulation, SRC promotes transformation progression. upregulation common sporadic inflammatory bowel disease-associated CRCs correlates with stage poor clinical outcome. Inhibition of CRC mouse models reduces growth...
Schmallenberg virus (SBV) is an emerging orthobunyavirus of ruminants associated with outbreaks congenital malformations in aborted and stillborn animals. Since its discovery November 2011, SBV has spread very rapidly to many European countries. Here, we developed molecular serological tools, experimental vivo model as a platform study pathogenesis, tropism virus-host cell interactions. Using synthetic biology approach, reverse genetics system for the rapid rescue genetic manipulation SBV....
Abstract The multigenic nature of human tumours presents a fundamental challenge for cancer drug discovery. Here we use Drosophila to generate 32 models colon using patient data from Cancer Genome Atlas. These recapitulate key features cancer, often as emergent properties combinations. Multigenic such ras p53 pten apc exhibit resistance panel cancer-relevant drugs. Exploring one in detail, identify mechanism the PI3K pathway inhibitor BEZ235. We this combinatorial therapy that circumvents...
Jaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary carcinoma, a unique animal model for human bronchioalveolar carcinoma. We previously isolated JSRV proviral clone and showed that it was both infectious oncogenic. Thus necessary sufficient development but no data are available on mechanisms transformation. Inspection genome reveals standard retroviral genes, evidence viral oncogene. However, an alternate ORF in pol (orf-x) might be candidate transforming gene....
Objective Transcribed-ultraconserved regions (T-UCR) are long non-coding RNAs which conserved across species and involved in carcinogenesis. We studied T-UCRs downstream of the Wnt/β-catenin pathway liver cancer. Design Hypomorphic Apc mice ( Apcfl/fl ) thiocetamide (TAA)-treated rats developed dependent hepatocarcinoma (HCC) cholangiocarcinoma (CCA), respectively. T-UCR expression was assessed by microarray, real-time PCR situ hybridisation. Results Overexpression uc.158− could...
ABSTRACT Jaagsiekte sheep retrovirus (JSRV) is the causative agent of a transmissible lung cancer known as ovine pulmonary carcinoma. Recently, we have found that expression JSRV envelope (Env) sufficient to transform mouse NIH 3T3 cells in classical transformation assays. To further investigate mechanisms oncogenesis, generated series chimeras between and JSRV-related endogenous retroviruses (enJSRVs) assessed them Chimeras containing exogenous SU region enJSRV TM were unable cells....
ABSTRACT Integrated into the sheep genome are 15 to 20 copies of type D endogenous loci that highly related two exogenous oncogenic viruses, jaagsiekte retrovirus (JSRV) and enzootic nasal tumor virus (ENTV). The viruses cause infectious neoplasms respiratory tract in small ruminants. In this study, we molecularly cloned three intact retroviruses (enJS56A1, enJS5F16, enJS59A1; collectively called enJRSV s) analyzed their genomic structures, phylogenies with respect counterparts, capacity...
Recent studies have suggested increased plasticity of differentiated cells within the intestine to act both as intestinal stem (ISCs) and tumour-initiating cells. However, little is known processes that regulate this plasticity. Our previous work has shown activating mutations Kras or NF-κB pathway can drive dedifferentiation lacking Apc. To investigate process further, we profiled undergoing in vitro tumours generated from these vivo by gene expression analysis. Remarkably, no clear...
ABSTRACT Jaagsiekte sheep retrovirus (JSRV) is the etiologic agent of a contagious bronchioloalveolar carcinoma known as pulmonary adenomatosis (SPA; ovine carcinoma). JSRV unique among retroviruses because it transforms alveolar type II cells and nonciliated bronchiolar (Clara cells) lungs; these are where specifically expressed in both naturally experimentally SPA-affected sheep. In this study, we investigated cell specificity expression. By transient-transfection assays 23 different lines...
Mycobacteriosis is a common and persistent bacterial disease affecting cultured, wild pet fish. The can be caused by various Mycobacterium spp. Currently, depopulation disinfection are the main recommended measures for containing outbreaks, as no vaccines commercially available, only few reports of successful antimicrobial therapies have been made. While disinfectant susceptibility studies conducted on planktonic forms some non-tuberculous mycobacteria (NTM) fish, biofilm-related research...
The 4662 KPC model is one of the most widely used mouse models pancreatic cancer. It represents an excluded immune phenotype and closely recapitulates pathophysiology cancer in humans. We set out to identify endogenous neoepitopes present cells. By combining whole-exome RNA-sequencing a bioinformatic neoantigen prediction pipeline, we have identified 15 potential candidate epitopes. Ten more highly expressed were selected for validation vivo vaccination study with 4662-tumor bearing mice....
ABSTRACT Ovine betaretroviruses include Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV). JSRV ENTV represent a unique class of oncogenic retroviruses that induce tumors the respiratory tract. are highly related but different diseases. Expression envelope (Env) induces transformation rodent fibroblasts in vitro phosphorylation Akt, central player phosphatidylinositol 3-kinase (PI-3K)/Akt signal transduction pathway. However, little information is available on...
Retroviruses use different strategies to regulate transcription and translation exploit the cellular machinery involved in these processes. This study shows that signal peptide of envelope glycoprotein (Env) Jaagsiekte sheep retrovirus (JSRV) plays a major role posttranscriptional viral gene expression. Expression JSRV Env trans increases particle production by mechanisms dependent on (i) its leader sequence, (ii) an intact cleavage site, (iii) cis-acting RNA-responsive element located...
Jaagsiekte sheep retrovirus (JSRV) is a unique oncogenic virus with distinctive biological properties. JSRV the only causing naturally occurring lung cancer (ovine pulmonary adenocarcinoma, OPA) and possessing major structural protein that functions as dominant oncoprotein. Lung cause of death among patients. OPA can be an extremely useful animal model in order to identify cells originating adenocarcinoma study early events carcinogenesis. In this study, we demonstrated originates from...
Abstract Junctional adhesion molecules (JAMs) play a critical role in cell permeability, polarity and migration. JAM-A, key protein of the JAM family, is altered number conditions including cancer; however, consequences JAM-A dysregulation on carcinogenesis appear to be tissue dependent organ with significant implications for use as biomarker or therapeutic target. Here, we test expression prognostic downregulation primary metastatic colorectal cancer (CRC) ( n = 947). We show that observed...
Histopathology, the gold-standard technique in classifying canine mammary tumors (CMTs), is a time-consuming process, affected by high inter-observer variability. Digital (DP) and Computer-aided pathology (CAD) are emergent fields that will improve overall classification accuracy. In this study, ability of CAD systems to distinguish benign from malignant CMTs has been explored on dataset-namely CMTD-of 1056 hematoxylin eosin JPEG images 20 24 CMTs, with three different based combination...
The exogenous and pathogenic Jaagsiekte sheep retrovirus (JSRV) coexists with highly related biologically active endogenous retroviruses (enJSRVs). enJS56A1 locus possesses a defective Gag polyprotein which blocks the late replication steps of by mechanism known as JSRV restriction (JLR). Conversely, enJSRV-26, most likely integrated into genome less than 200 years ago, is able to escape JLR. In this study, we demonstrate that ability enJSRV-26 JLR due single-amino-acid substitution in...
The genus Anaplasma (Anaplasmataceae, Rickettsiales) includes tick-transmitted bacterial species of importance to both veterinary and human medicine. Apart from the traditionally recognized six (A. phagocytophilum, A. platys, bovis, ovis, centrale, marginale), novel strains candidate species, also relevance medicine, are emerging worldwide. Although related zoonotic platys phagocytophilum have been reported in several African European Mediterranean countries, data on presence these...
Understanding the factors governing host species barriers to virus transmission has added significantly our appreciation of pathogenesis. Jaagsiekte sheep retrovirus (JSRV) is causative agent ovine pulmonary adenocarcinoma (OPA), a transmissible lung cancer that rarely been found in goats. In this study, order further clarify pathogenesis OPA, we investigated whether goats are resistant JSRV replication and carcinogenesis. We induces tumors with macroscopic histopathological features...
Bone marrow stromal cell antigen 2 (BST2) is a cellular restriction factor with broad antiviral activity. In sheep, the BST2 gene duplicated into two paralogs termed oBST2A and oBST2B. impedes viral exit of Jaagsiekte sheep retroviruses (JSRV), most probably by retaining virions at membrane, similar to "tethering" mechanism exerted human BST2. this study, we provide evidence that unlike oBST2A, oBST2B limited Golgi apparatus disrupts JSRV envelope (Env) trafficking sequestering it. turn,...