A5014820649- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- interferon and immune responses
- Virus-based gene therapy research
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Nanocluster Synthesis and Applications
- Nuclear Receptors and Signaling
- Advanced Nanomaterials in Catalysis
- Dendrimers and Hyperbranched Polymers
- Optical Imaging and Spectroscopy Techniques
- Hedgehog Signaling Pathway Studies
- Cell death mechanisms and regulation
- Regulation of Appetite and Obesity
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Polymer Surface Interaction Studies
- Photoacoustic and Ultrasonic Imaging
- Renal and related cancers
Universidade de Santiago de Compostela
2016-2025
Center for Research in Molecular Medicine and Chronic Diseases
2015-2025
Instituto de Investigación Sanitaria de Santiago
2012-2021
Centro de Investigación Biomédica en Red
2017
Marqués de Valdecilla University Hospital
2017
Instituto de Investigación Marqués de Valdecilla
2017
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2017
Centro Tecnológico del Mar
2009
Memorial Sloan Kettering Cancer Center
2000-2007
New York University
2006
Abstract Compounds with specific cytotoxic activity in senescent cells, or senolytics, support the causal involvement of senescence aging and offer therapeutic interventions. Here we report identification Cardiac Glycosides (CGs) as a family compounds senolytic activity. CGs, by targeting Na+/K+ATPase pump, cause disbalanced electrochemical gradient within cell causing depolarization acidification. Senescent cells present slightly depolarized plasma membrane higher concentrations H+, making...
Ghrelin is a stomach-derived peptide that increases food intake through the activation of hypothalamic AMP-activated protein kinase (AMPK). However, molecular mechanisms initiated by ghrelin receptor, which in turn lead to AMPK activation, remain unclear. Sirtuin 1 (SIRT1) deacetylase activated response calorie restriction acts tumor suppressor gene p53. We tested hypothesis central SIRT1/p53 pathway might be mediating orexigenic action ghrelin.SIRT1 inhibitors, such as Ex527 and sirtinol,...
The mechanisms responsible for the transcriptional silencing of pluripotency genes in differentiated cells are poorly understood. We have observed that lacking tumor suppressor p27 can be reprogrammed into induced pluripotent stem (iPSCs) absence ectopic Sox2. Interestingly, and tissues from null mice, including brain, lung, retina, present an elevated basal expression Sox2, suggesting contributes to repression Furthermore, iPSCs fail fully repress Sox2 upon differentiation. Mechanistically,...
Abstract p53 regulates several signaling pathways to maintain the metabolic homeostasis of cells and modulates cellular response stress. Deficiency or excess nutrients causes stress, we hypothesized that could be linked glucose maintenance. We show here upon starvation hepatic is stabilized by O -GlcNAcylation plays an essential role in physiological regulation homeostasis. More specifically, binds PCK1 promoter its transcriptional activation, thereby controlling production. Mice lacking...
Abstract Despite therapeutic advancements, oral cavity squamous cell carcinoma (OCSCC) remains a difficult disease to treat. Systemic platinum-based chemotherapy often leads dose-limiting toxicity (DLT), affecting quality of life. PRV111 is nanotechnology-based system for local delivery cisplatin loaded chitosan particles, that penetrate tumor tissue and lymphatic channels while avoiding systemic circulation toxicity. Here we evaluate using animal models cancer, followed by clinical trial in...
Abstract Super-resolution optoacoustic imaging of microvascular structures deep in mammalian tissues has so far been impeded by strong absorption from densely-packed red blood cells. Here we devised 5 µm biocompatible dichloromethane-based microdroplets exhibiting several orders magnitude higher optical than cells at near-infrared wavelengths, thus enabling single-particle detection vivo. We demonstrate non-invasive three-dimensional microangiography the mouse brain beyond acoustic...
Increased translation of p27 mRNA correlates with withdrawal cells from the cell cycle. This raised possibility that antimitogenic signals might mediate their effects on expression by altering complexes formed mRNA, regulating its translation. In this report, we identify a U-rich sequence in 5′ untranslated region (5′UTR) is necessary for efficient proliferating and nonproliferating cells. We show number factors bind to 5′UTR vitro manner dependent element, availability cytosol controlled...
Chitosan (CS) colloidal carriers, which consist of an oily core and a CS coating, were developed to facilitate controlled intracellular delivery docetaxel. The systems presented particle size <200 nm positive surface charge. As shown by the flow cytometry analysis, fluorescent carriers rapidly internalized human tumor cells. Fluorescence was observed in more than 80% MCF7 (human breast adenocarcinoma) almost 100% A549 lung carcinoma) cells when 2 h treatment with given. A total 24 after...
Abstract Cellular senescence is often considered a protection mechanism triggered by conditions that impose cellular stress. Continuous proliferation, DNA damaging agents or activated oncogenes are well-known activators of cell senescence. Apart from characteristic stable cycle arrest, this response also involves proinflammatory phenotype known as senescence-associated secretory (SASP). This, together with the widely interference pathways some oncoviruses, had led to hypothesis may be part...
Members of the cyclin-dependent kinase (CDK)-inhibitory protein (CIP)/kinase-inhibitory (KIP) family inhibitors regulate proliferation and cell cycle exit mammalian cells. In adult brain, CIP/KIP p27(kip1) has been related to regulation intermediate progenitor cells located in neurogenic niches. Here, we uncover a novel function hippocampus as dual regulator stem quiescence cell-cycle immature neurons. vivo, is detected radial expressing SOX2 newborn neurons dentate gyrus. vitro, Cdkn1b gene...
Ghrelin, a stomach-derived peptide, stimulates feeding behavior and adiposity. For its orexigenic action, ghrelin triggers central SIRT1/p53/AMPK pathway. The tumor suppressor p53 also plays an important role in white adipose tissue (WAT), where it is up-regulated the adipocytes of obese mice. It not known, however, whether has any mediating peripheral action ghrelin. In present study, chronic treatment resulted increased body weight fat-mass gain wild-type Correspondingly, mRNA levels...
The amount of p27<sup>Kip1</sup>establishes a threshold to which G<sub>1</sub>cyclin-cyclin-dependent kinase complexes must surpass prior cells progressing into S-phase. p27 is greatest in G<sub>0</sub> cells, intermediate G<sub>1</sub> and lowest S-phase cells. However, there little known regarding the pathways mechanisms controlling accumulation We report that inhibition Rho, by either lovastatin or C3 exoenzyme, can increase translational efficiency mRNA. Similar pharmacologic...