A5062574961- Immunotherapy and Immune Responses
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Nutrition, Genetics, and Disease
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Heat shock proteins research
- Hepatitis B Virus Studies
- Metabolism, Diabetes, and Cancer
- Salivary Gland Disorders and Functions
- Virus-based gene therapy research
- Bone Tissue Engineering Materials
- Metabolomics and Mass Spectrometry Studies
- Gastrointestinal motility and disorders
- HIV/AIDS Impact and Responses
- HIV-related health complications and treatments
- Antimicrobial Peptides and Activities
- Thermal Regulation in Medicine
- Fluoride Effects and Removal
- Pneumocystis jirovecii pneumonia detection and treatment
- Phagocytosis and Immune Regulation
- Systemic Lupus Erythematosus Research
Aboca (Italy)
2020-2025
Humanitas University
2015-2019
University of Turin
2007-2018
University of Perugia
2013
Fondazione Humanitas per la Ricerca
2009
The metabolic actions of the ghrelin gene-derived peptide obestatin are still unclear. We investigated effects in vitro, on adipocyte function, and vivo, insulin resistance inflammation mice fed a high-fat diet (HFD). Obestatin apoptosis, differentiation, lipolysis, glucose uptake were determined vitro mouse 3T3-L1 human subcutaneous (hSC) omental (hOM) adipocytes. In influence metabolism was assessed an HFD for 8 wk. 3T3-L1, hSC, hOM preadipocytes adipocytes secreted showed specific binding...
When the protective mechanisms of gastroesophageal mucosa are overwhelmed by injurious factors, structural and functional mucosal integrity is compromised, resulting in a wide spectrum disorders. Poliprotect has recently been shown to be non-inferior standard-dose omeprazole for treatment endoscopy-negative patients with heartburn and/or epigastric pain or burning. Here, we provide preclinical data describing mechanism action formulation, 100% natural, biodegradable, environmental friendly...
Abstract Changes in the homeostasis of tumor necrosis factor α (TNFα) have been demonstrated patients and experimental models amyotrophic lateral sclerosis (ALS). However, contribution TNFα to development ALS is still debated. expressed by glia neurons acts through membrane receptors TNFR1 TNFR2, which may opposite effects neurodegeneration. We investigated role its selective motor neuron death vitro vivo . TNFR2 astrocytes neurons, but not TNFR1, was implicated loss primary SOD1‐G93A...
// Stefano Garetto 1,* , Claudia Sardi Elisa Martini 1 Giuliana Roselli Diego Morone 2 Roberta Angioni Beatrice Cianciotti Anna Trovato Davide Giuseppe Franchina Giovanni Francesco Castino 3 Debora Vignali Marco Erreni Federica Marchesi 3,4 Cristiano Rumio 5 and Marinos Kallikourdis 1,6 Adaptive Immunity Laboratory, Humanitas Clinical Research Center, Rozzano (Milano), Italy Laboratory of Cellular Immunology, 4 Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli...
Abstract BACKGROUND Prostate hyperthermia and photodynamic therapy can be delivered by a variety of procedures which result in wide range temperatures light energy cause different kinds cell death. METHODS We have addressed the immunogenic effect heating UVC irradiation on prostate cancer (PCa) line LNCaP, studying release Danger Associated Molecule Pattern (DAMP) molecules HSP70 HMGB1 dendritic (DC) antigen‐presenting efficiency. RESULTS Intracellular upmodulation extracellular were...
Current pharmacological therapies for the management of chronic articular diseases are far from being satisfactory, so new strategies need to be investigated. We tested intra-articular pain relieving properties a system molecules characterized Centella asiatica extract (14G1862) in rat model osteoarthritis induced by monoiodoacetate (MIA). 14G1862 (0.2–2 mg mL−1) was intra-articularly (i.a.) injected 7 days after MIA, behavioural and histological evaluations were performed 14, 30 60...
Regulatory T cells (Treg) influence the development of autoimmunity and their use is increasingly proposed for clinical applications. The well-characterized suppressive potential Treg frequently leads to assumption that presence in prevailing numbers indicative immunosuppression. We hypothesized this may be false. examined models three different diseases caused by organ-specific autoimmune responses: primary biliary cirrhosis, atherosclerosis rheumatoid arthritis (RA). indicators relative...
Vitamin D (VD) has been used to prevent nonalcoholic fatty liver disease (NAFLD), a condition of lipotoxicity associated with defective metabolism and function this vitamin. Different forms VD are available can be for scope, but their effects on cell remain unexplored. In study we compared natural formulation rich in VD2 (Shiitake Mushroom extract or SM-VD2) synthetic containing pure VD3 (SV-VD3) the bioactive metabolite 1,25(OH)2-D3. These were investigated chemoprevention mode human HepaRG...
It is generally accepted that salivary components are important for dental health, but to date no clear correlation has been found between one or more of these and the outcome caries. The identification factors preventing, favoring signaling caries might help control disease. In present study, western blotting analysis whole saliva from 20 healthy caries‐free children showed presence soluble form CD14, a bacterial pattern‐recognition receptor many involved in innate immune response. identity...
Abstract Metabolic syndrome has increased at a worrisome level. Lifestyle changes are not sufficient to prevent and improve the adverse effects of obesity, thus novel interventions necessary. The aim this study was investigate use metabolic outcomes non-pharmacological intervention in high-fat diet (HFD) fed mouse model, capable recapitulating key aspects syndrome. We show that Policaptil Gel Retard remarkable, beneficial on dysfunction caused by consumption HFD. describe mechanism which...
The evolution of medical devices has led to the introduction that include "substances" and which, due their presentation sites application may resemble medicinal products. difference between substance-based products lies in proper definition principal mechanism action. major problem at moment is lack a procedure for demonstration "not pharmacological, immunological or metabolic". We aimed design an experimental set up demonstrate action two substances used commonly treatment constipation,...
Wheat germ oil (WGO) is rich in α-tocopherol (vitamin E, VE), a vitamin that has long been suggested to exert hepatoprotective effects. In this study, function of WGO-VE and its transcriptomics fingerprint were investigated comparison with RRR−α-tocopherol all-rac-α-tocopherol (nVE sVE, respectively), human liver cells treated oleic acid (OA) develop steatosis lipotoxicity. Used chemoprevention mode, all the VE formulations afforded significant reduction OA-induced consequent impact on...
Abstract Dendritic cells (DCs) are highly specialized antigen-presenting endowed with the unique ability to not only present exogenous antigens upon exposure MHC II, but also cross-present these I. This property was exploited generate tumor-specific CD8 cytotoxic lymphocyte (CTL) response in DCs-based cancer vaccine protocols. In this context, source of tumor remains a critical challenge. A crude context danger signals is believed represent an efficient (TAs) for DCs loading. our previous...
Wheat germ is a main natural source of vitamin E (VE). This (as α-tocopherol) has long been suggested to afford hepatoprotection effects. In this study, function and its transcriptomics fingerprint were investigated in wheat oil (WGO)-VE using human liver cells treated with oleic acid (OA) develop steatosis lipotoxicity. WGO-VE was used chemoprevention mode compared RRR-α-tocopherol all-rac-α-tocopherol VE (nVE sVE, respectively). nVE more efficient sVE reducing the OA-induced consequent...