A5100369083- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ferroptosis and cancer prognosis
- Genetic Syndromes and Imprinting
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- RNA Research and Splicing
- dental development and anomalies
- Renal and related cancers
- Reproductive Biology and Fertility
- Cancer Genomics and Diagnostics
- Gestational Diabetes Research and Management
- Mechanisms of cancer metastasis
- Immune cells in cancer
- Hedgehog Signaling Pathway Studies
- Redox biology and oxidative stress
- Gene Regulatory Network Analysis
- Developmental Biology and Gene Regulation
- Hemoglobinopathies and Related Disorders
- Connective tissue disorders research
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2025
University of Pennsylvania
2017-2024
California Institute for Regenerative Medicine
2024
Binzhou Medical University
2024
Binzhou University
2024
China Agricultural University
2008-2023
Beijing YouAn Hospital
2018-2023
Capital Medical University
2023
Kunming Medical University
2019-2023
Xiangya Hospital Central South University
2021
Human aging cannot be fully understood in terms of the constrained genetic setting. Epigenetic drift is an alternative means explaining age-associated alterations. To address this issue, we performed whole-genome bisulfite sequencing (WGBS) newborn and centenarian genomes. The DNA had a lower methylation content reduced correlation status neighboring cytosine--phosphate--guanine (CpGs) throughout genome comparison with more homogeneously methylated DNA. hypomethylated CpGs observed compared...
DNA methylation plays an important role in biological processes human health and disease. Recent technological advances allow unbiased whole-genome (methylome) analysis to be carried out on cells. Using bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome of the unique sequences peripheral blood mononuclear cells (PBMC) from same Asian individual whose genome was deciphered YH project. PBMC constitute source for clinical tests...
FBP1, fructose-1,6-bisphosphatase-1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to 6-phosphate and inorganic phosphate. The mechanism that it functions antagonize glycolysis was epigenetically inactivated through NF-kappaB pathway in gastric cancer has been reported. However, its role liver carcinogenesis still remains unknown. Here, we investigated expression DNA methylation FBP1 primary HCC colon tumor. lowly expressed 80% (8/10) human...
The idea that changes to the host immune system are critical for cancer progression was proposed a century ago and recently regained experimental support. Herein, hypothesis hepatocellular carcinoma (HCC) leaves molecular signature in peripheral tested by profiling DNA methylation blood mononuclear cells (PBMC) T from discovery cohort (n = 69) of healthy controls, chronic hepatitis, HCC using Illumina 450K platform validated two validation sets 80 n 48) pyrosequencing. study reveals broad...
The Crest phenotype is characterised by a tuft of elongated feathers atop the head. A similar also seen in several wild bird species. shows an autosomal incompletely dominant mode inheritance and associated with cerebral hernia. Here we show, using linkage analysis genome-wide association, that located on E22C19W28 group it complete association to HOXC-cluster this chromosome. Expression tissues from Crested non-crested chickens, representing 26 different breeds, revealed HOXC8, but not...
Glutaredoxins (GRXs) are small proteins which bind glutathione to either reduce disulfide bonds or coordinate iron sulfur clusters. Whereas these well-established functions associated with ubiquitously occurring GRXs that encode variants of a CPYC CGFS motif in the active center, land plants also possess CCxC/S-type (named ROXYs) for biochemical yet unknown. ROXYs physically and genetically interact bZIP transcription factors TGA family. In Arabidopsis, ectopically expressed ROXY19...
DNA cytosine methylation is an important epigenetic modification that has significant effects on a variety of biological processes in animals. Avian species hold crucial position evolutionary history. In this study, we used whole-genome bisulfite sequencing (MethylC-seq) to generate single base profiles lungs two genetically distinct and highly inbred chicken lines (Fayoumi Leghorn) differ genetic resistance multiple pathogens, explored the potential regulatory role associated with immune...
ABSTRACT Objective To compare survival outcomes between patients with ovarian cancer platinum‐sensitive recurrence (PSR) who underwent secondary cytoreductive surgery (SCS) at the first and those SCS second recurrence. Design Multicentre cohort study. Setting Data were retrospectively collected from five hospitals in China. Population Patients either or PSR. Methods Overall (OS) time to progression (PFS2), calculated of next following SCS, compared groups. Postsurgical complications...
To investigate the effects of histone methyltransferase ESET (also known as SETDB1) on bone metabolism, we analyzed osteoblasts and osteoclasts in knockout animals, performed osteogenesis assays using ESET-null mesenchymal stem cells. We found that deletion severely impairs osteoblast differentiation but has no effect osteoclastogenesis, co-transfection represses Runx2-mediated luciferase reporter while siRNA knockdown activates cells, is required for postnatal expression Indian hedgehog...
The chromobox protein homolog 7 (CBX7), one member of the polycomb group family, has been characterized mainly to play a tumor-suppressive role in human malignant neoplasias. Moreover, downregulation CBX7 is correlated with poor prognosis and aggressiveness variety cancers. However, biological functions cervical cancer have not elucidated. In present study, we explore whether exerts its function cancer. To achieve this goal, molecular approaches were used upregulate expression or cell lines....
Clear cell renal carcinoma (ccRCC) accounts for 80% of all cancers and has a poor prognosis. Chromobox (CBX) family protein expression been reported in variety human malignancies, but the roles CBXs ccRCC remain unclear. In this study, by using ONCOMINE, UALCAN, GEPIA, Kaplan-Meier Plotter, cBioPortal, TIMER, we found transcriptional levels CBX3 CBX4 tissues were significantly higher than those normal kidney tissues, whereas CBX1, CBX5, CBX6, CBX7 reduced tissues. The promoters CBX2, CBX3,...
DNA methylation is a widely studied epigenetic mechanism known to correlate with gene repression and genomic stability. Development of sensitive methods for global detection events particular importance. We here describe technique, called modified methylation-specific digital karyotyping (MMSDK) based on (MSDK) novel sequencing approach. Briefly, after tandem digestion methylation-sensitive mapping enzyme fragmenting enzyme, short sequence tags are obtained. These amplified, followed by...
SOX1 is epigenetically inactivated in hepatocellular carcinoma. However, the expression and methylation status of non-small cell lung cancer (NSCLC) remains unknown. The aim current study was to investigate whether promoter hypermethylation involved human carcinogenesis. We first detected protein a tissue microarray (TMA) primary NSCLC adjacent normal specimens using immunohistochemical staining with specific anti-SOX1 antibody. Methylation region tissues determined by bisulfite sequencing...
Cyclin-dependent kinase inhibitor 2A (CDKN2A) gene methylation has been paramount in the development of malignant masses. The purpose conducted research was to evaluate mechanism and involvement regards CDKN2A specific locus region gastric cancer (GC) with comprehensive statistical analysis utilizing statistics acquired from Cancer Genome Atlas (TCGA) database. Gene Set Enrichment Analysis (GSEA) revealed that level its GC tissues increased compared para-cancerous tissues. In multivariate...
Avian eggshell color is an interesting genetic trait. Here, we report that the blue of domestic duck caused by two cis -regulatory G to A transitions upstream ABCG2 , which encodes efflux transporter. The juxtaposed allele A-A exhibited higher promoter activity and stronger nuclear protein binding capacity than white G-G. Transcription factor analysis suggested differential capability CTCF between alleles. Knockdown expression significantly decreased but not allele. DNA methylation revealed...
To obtain reliable results in quantitative PCR (qPCR) reactions, an endogenous control (EC) gene is needed to correct for systematic variations. In this study, a TaqMan low density array was used quantify the expression levels of microRNA (miRNA) genes vivo fertilized, vitro parthenogenetic and somatic cell nuclear transfer blastocysts. The aim identify suitable EC qPCR analysis miRNAs porcine showed that thirty-six were commonly expressed four kinds embryos eleven similar different embryo...
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Although we had identified that the methylation of AHNAK was a good diagnostic marker for hepatopathy, here speculate there also another marker, STAP1, whose involved in detection hepatopathy.We investigated levels and STAP1 peripheral blood mononuclear cells chronic hepatitis B (CHB) patients, compensatory liver cirrhosis (CLC) decompensated (DCLC) hepatocellular carcinoma (HCC) patients healthy controls by methylation-specific PCR. We evaluated differences changes expression at different...